Polyphasic taxonomy

多相分类学
  • 文章类型: Journal Article
    革兰氏染色阴性,浅卡其色,严格的有氧,杆状,通过多个鞭毛运动,过氧化氢酶和氧化酶阳性细菌,指定为SSM4.3T,是从东海沟旗岛的海藻中分离出来的。新型分离物在0-5.0%NaCl浓度(w/v)(最佳1%)下生长,pH5.0-9.0(最佳pH7.0),和15-37°C(最佳30°C)。基于16SrRNA基因序列的系统发育表明,该新型海洋分离株属于根瘤菌科,与PeteryoungiarhighzophilaCGMCC1.15691T具有最大的序列相似性(98.9%)。这种分类也得到了使用核心基因的系统发育分析的支持。该菌株的主要脂肪酸(≥10%)被鉴定为C18:1ω7c/C18:1ω6c。Q-10被确定为主要的类异戊二烯醌,有微量的Q-9存在。主要极性脂质被鉴定为二磷脂酰甘油,磷脂酰乙醇胺和磷脂酰甘油。菌株SSM4.3T的完整基因组大小为4.39Mb,DNAGC含量为61.3%。平均核苷酸同一性,数字DNA-DNA杂交,菌株SSM4.3T及其密切相关的代表的基因组之间的平均氨基酸同一性值为74.80-86.93%,20.00-32.30%,和70.30-91.52%,分别。系统发育分析,以核心基因为基础,揭示了SSM4.3T与其他Peteryoungia菌株之间的进化关系。对8种先前分类的Peteryoungia物种和SSM4.3T进行的泛基因组学分析揭示了它们独特的遗传特征和功能。总的来说,菌株SSM4.3T被认为是Peteryoungia属的新物种;名称为Peteryoungia藻类sp。11月。已经被提议,与SSM4.3T型菌株(=LMG32561=MCCC1K07170)。
    A Gram-stain-negative, light khaki, strictly aerobic, rod-shaped, motile via multiple flagella, and catalase- and oxidase-positive bacterium, designated as SSM4.3T, was isolated from the seaweed of Gouqi Island in the East China Sea. The novel isolate grows at 0-5.0% NaCl concentrations (w/v) (optimum 1%), pH 5.0-9.0 (optimum pH 7.0), and 15-37 °C (optimum 30 °C). The 16S rRNA gene sequences-based phylogeny indicates that the novel marine isolate belongs to the family Rhizobiaceae and that it shared the greatest sequence similarity (98.9%) with Peteryoungia rhizophila CGMCC 1.15691T. This classification was also supported by phylogenetic analysis using core genes. The predominant fatty acids (≥ 10%) of the strain were identified as C18:1 ω7c/C18:1 ω6c. Q-10 was identified as the major isoprenoid quinone, with trace levels of Q-9 present. The major polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The complete genome size of strain SSM4.3T is 4.39 Mb with a DNA G+C content of 61.3%. The average nucleotide identity, digital DNA-DNA hybridization, and average amino acid identity values between the genomes of strain SSM4.3T and its closely related representatives were 74.80-86.93%, 20.00-32.30%, and 70.30-91.52%, respectively. Phylogenetic analysis, grounded on the core genes, reveals the evolutionary relationship between SSM4.3T and other Peteryoungia strains. Pan-genomics analysis of 8 previously classified Peteryoungia species and SSM4.3T revealed their unique genetic features and functions. Overall, strain SSM4.3T was considered to be a new species of the Peteryoungia genus; the name Peteryoungia algae sp. nov. has been proposed, with type strain SSM4.3T (= LMG 32561 = MCCC 1K07170).
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  • 文章类型: Journal Article
    两个新颖的杆状,严格的有氧,非运动和革兰氏染色阴性细菌菌株,指定为SDUM040013T和SDUM040014T,从威海的海带幼苗中分离出来,公关中国。菌株SDUM040013T的细胞宽0.3-0.4µm,长0.8-1.8µm,过氧化氢酶阳性和氧化酶阳性。在0-37°C下观察到SDUM040013T的生长(最佳,28-30°C)和pH5.5-9(最佳,pH8.0),并且在1-8%(w/v)NaCl存在下(最佳,2%)。菌株SDUM040013T的DNAG+C含量为50.5%。菌株SDUM040013T与Gilvimarinuschinensis表现出最高的16SrRNA基因序列相似性(97.1%)。菌株SDUM040014T的细胞宽0.4-0.5µm,长1.0-1.4µm,过氧化氢酶阳性和氧化酶阳性。在4-40°C下观察到SDUM040014T的生长(最佳,28-30°C)和pH5.5-9(最佳,pH8.5)和存在0-8%(w/v)NaCl(最佳,2%)。菌株SDUM040014T的DNAG+C含量为56.5%。菌株SDUM040014T与溶多糖Gilvimarinus显示出最高的16SrRNA基因序列相似性(96.2%)。两种菌株的类异戊二烯醌均为Q-8,主要脂肪酸为特征3(C16:1ω7c和/或C16:1ω6c),求和特征8(C18:1ω7c)和C16:0。二磷脂酰甘油,磷脂酰甘油和磷脂酰乙醇胺是主要的极性脂质。鉴于这些表型和化学分类学特性,以及系统发育数据,菌株SDUM040013T和SDUM040014T被认为代表了Gilvimarinus属的两个新物种,为此,名字Gilvimarinusgilvussp。11月。和Gilvimarinus藻类sp。11月。是提议的。菌株类型为SDUM040013T(=KCTC8123T=MCCC1H01413T)和SDUM040014T(=KCTC8124T=MCCC1H0141414T),分别。
    Two novel rod-shaped, strictly aerobic, non-motile and Gram-stain-negative bacterial strains, designated SDUM040013T and SDUM040014T, were isolated from kelp seedlings in Weihai, PR China. Cells of strain SDUM040013T were 0.3-0.4 µm wide and 0.8-1.8 µm long, catalase-positive and oxidase-positive. Growth of SDUM040013T was observed at 0-37 °C (optimum, 28-30 °C) and pH 5.5-9 (optimum, pH 8.0) and in the presence of 1-8 % (w/v) NaCl (optimum, 2 %). The DNA G+C content of strain SDUM040013T was 50.5 %. Strain SDUM040013T showed the highest 16S rRNA gene sequence similarity (97.1 %) to Gilvimarinus chinensis. Cells of strain SDUM040014T were 0.4-0.5 µm wide and 1.0-1.4 µm long, catalase-positive and oxidase-positive. Growth of SDUM040014T was observed at 4-40 °C (optimum, 28-30 °C) and pH 5.5-9 (optimum, pH 8.5) and in the presence of 0-8 % (w/v) NaCl (optimum, 2 %). The DNA G+C content of strain SDUM040014T was 56.5 %. Strain SDUM040014T showed the highest 16S rRNA gene sequence similarity (96.2%) to Gilvimarinus polysaccharolyticus. The isoprenoid quinone of both strains was Q-8 and the predominant fatty acids were summed feature 3 (C16 : 1  ω7c and/or C16 : 1  ω6c), summed feature 8 (C18 : 1  ω7c) and C16 : 0. Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine were the major polar lipids. Given these phenotypic and chemotaxonomic properties, as well as phylogenetic data, strains SDUM040013T and SDUM040014T were considered to represent two novel species of the genus Gilvimarinus, for which the names Gilvimarinus gilvus sp. nov. and Gilvimarinus algae sp. nov. are proposed. The type strains are SDUM040013T (=KCTC 8123T=MCCC 1H01413T) and SDUM040014T (=KCTC 8124T=MCCC 1H01414T), respectively.
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  • 文章类型: Journal Article
    从阿塔卡马沙漠的地下土壤中分离出几种菌株,并先前被分配给小单孢菌属。设计了一项多相研究,以确定分离株4G51T的分类隶属度,4G53,和4G57。所有菌株均显示出符合小单孢菌属分类的化学分类学特性,包括细胞壁肽聚糖中的内消旋二氨基庚二酸,MK-9(H4)作为主要的呼吸醌,iso-C15:0和iso-C16:0作为主要脂肪酸和二磷脂酰甘油,磷脂酰乙醇胺和磷脂酰肌醇为主要极性脂质。菌株4G51T的16SrRNA基因序列,4G53和4G57与小单孢菌CS1-12T型菌株的相似性最高(97.9%),在系统发育基因树中形成一个独立的分支。基因组系统发育证实了它们的独立位置,与kangleipakensis小单孢菌的类型菌株最密切相关。分离物与其最接近的系统基因组邻居之间的数字DNA-DNA杂交和平均核苷酸同一性分析证实,应将它们分配给小单孢菌属中的一个新物种,该物种的名称为小单孢菌。11月。(4G51T=PCM3031T=LMG30756T)提出。
    Several strains were isolated from subsurface soil of the Atacama Desert and were previously assigned to the Micromonospora genus. A polyphasic study was designed to determine the taxonomic affiliation of isolates 4G51T, 4G53, and 4G57. All the strains showed chemotaxonomic properties in line with their classification in the genus Micromonospora, including meso-diaminopimelic acid in the cell wall peptidoglycan, MK-9(H4) as major respiratory quinone, iso-C15:0 and iso-C16:0 as major fatty acids and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids. The 16S rRNA gene sequences of strains 4G51T, 4G53, and 4G57 showed the highest similarity (97.9 %) with the type strain of Micromonospora costi CS1-12T, forming an independent branch in the phylogenetic gene tree. Their independent position was confirmed with genome phylogenies, being most closely related to the type strain of Micromonospora kangleipakensis. Digital DNA-DNA hybridization and average nucleotide identity analyses between the isolates and their closest phylogenomic neighbours confirmed that they should be assigned to a new species within the genus Micromonospora for which the name Micromonospora sicca sp. nov. (4G51T=PCM 3031T=LMG 30756T) is proposed.
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  • 文章类型: Journal Article
    酚酸(PA)是在各种环境中广泛分布的化感物质。为了更好地理解PA在环境中的命运,从根际土壤中分离出的耐盐PAs降解细菌(命名为RR2S18T)被鉴定为Devosia的新物种,命名为Devosiarhizosphaeraesp.11月。该菌株最初使用CoA依赖性非β-氧化途径将PAs降解为中心环裂变中间体(原儿茶酸)。然后通过邻位裂解反应和β-酮己二酸途径连续降解产生的环裂变中间体。对62个Devosia菌株的比较基因组学分析表明,PAs降解基因普遍存在于其基因组中,表明PA降解在该属的成员中是普遍的。分析还表明,参与CoA依赖性非β-氧化的基因是Devosia菌株固有的,而参与环裂变和β-酮己二酸途径的分子是通过水平基因转移获得的。
    Phenolic acids (PAs) are widely distributed allelochemicals in various environments. To better understand the fate of PAs in environments, a halotolerant PAs-degrading bacterium (named strain RR2S18T) isolated from rhizosphere soil was identified as a novel species of Devosia, named Devosia rhizosphaerae sp. nov. The strain initially degraded PAs into central ring-fission intermediates (protocatechuic acid) using the CoA-dependent non-β-oxidation pathway. The produced ring-fission intermediates were then consecutively degraded by an ortho-cleavage reaction and the β-ketoadipic acid pathway. A comparative genomics analysis of 62 Devosia strains revealed that PAs-degrading genes were ubiquitous in their genomes, indicating that PAs degradation is universal among members of this genus. The analysis also suggested that the genes involved in CoA-dependent non-β-oxidation are inherent to Devosia strains, while those involved in ring-fission and β-ketoadipic acid pathways were obtained by horizontal gene transfer.
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  • 文章类型: Journal Article
    一种新型杆状细菌,从新疆库姆塔格沙漠收集的沙质土壤中分离出形成淡黄色菌落的菌株SYSUD60015T,中国。细胞革兰氏染色阴性,氧化酶阳性,过氧化氢酶阴性,具有单个极性鞭毛。生长最佳温度发生在28至37°C之间,pH7.0和0-0.5%(W/V)NaCl。主要的细胞脂肪酸(>5%)为特征8(C18:1ω7c和/或C18:1ω6c),C19:0循环ω8c,C18:1ω7c11-甲基和C16:0。极性脂质谱含有一种磷脂酰乙醇胺,一种二磷脂酰甘油,一种磷脂酰甘油,一种身份不明的磷脂,三种身份不明的氨基脂类,两种身份不明的氨基磷脂和七种身份不明的脂质。唯一的呼吸醌是泛醌-10。基于16SrRNA基因序列系统进化分析,发现SYSUD60015T菌株在Sneathiathellaceae家族中形成了独特的谱系,16SrRNA基因序列相似性为90.8%,和90.2%的反硝化费菌S3T。SYSUD60015T的基因组大小为5.66Mb,DNAGC含量为68.2%。低数字DNA-DNA杂交(dDDH,18.0%),平均核苷酸同一性(ANI,77.5%)和氨基酸同一性(AAI,SYSUD60015T和TerrovibrioK5T之间的56.0%)值表明SYSUD60015T可能代表一个不同的属。基于系统发育,表型,化学和基因组数据,我们提出了地黄杆菌。11月。,sp.11月。作为Sneathiathiellaceae家族中一个新属的新物种。菌株类型为SYSUD60015T(=NBRC112952T=CGMCC1.16256T)。
    A novel rod-shaped bacterium, designated as strain SYSU D60015T that formed yellowish colonies was isolated from a sandy soil collected from the Kumtag Desert in Xinjiang, China. Cells were Gram-stain-negative, oxidase-positive, catalase-negative and motile with a single polar flagellum. Growth optimum occurred between 28 and 37 °C, pH 7.0 and with 0-0.5% (W/V) NaCl. The predominant cellular fatty acids (> 5%) were summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), C19:0 cyclo ω8c, C18:1 ω7c 11-methyl and C16:0. The polar lipid profile contained one phosphatidylethanolamine, one diphosphatidylglycerol, one phosphatidylglycerol, one unidentified phospholipid, three unidentified aminolipids, two unidentified aminophospholipids and seven unidentified lipids. The only respiratory quinone was ubiquinone-10. Based on 16S rRNA gene sequence phylogenetic analysis, strain SYSU D60015T was found to form a distinct linage within the family Sneathiellaceae, and had 16S rRNA gene sequence similarities of 90.8% to Taonella mepensis H1T, and 90.2% to Ferrovibrio denitrificans S3T. The genome of SYSU D60015T was 5.66 Mb in size with 68.2% of DNA G + C content. The low digital DNA-DNA hybridization (dDDH, 18.0%), average nucleotide identity (ANI, 77.5%) and amino acid identity (AAI, 56.0%) values between SYSU D60015T and Ferrovibrio terrae K5T indicated that SYSU D60015T might represent a distinct genus. Based on the phylogenetic, phenotypic, chemotaxonomic and genomic data, we propose Desertibaculum subflavum gen. nov., sp. nov. as a novel species of a new genus within the family Sneathiellaceae. The type strain is SYSU D60015T (= NBRC 112952T = CGMCC 1.16256T).
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  • 文章类型: Journal Article
    对从突尼斯Retamaraetam根瘤中分离出的IRAMC:0171T菌株进行了整合分类基因组标准的全面多相分类学研究。这种革兰氏染色阴性和需氧细菌在5-45°C的温度范围内繁殖,最佳温度为28°C,和0-6%NaCl的耐受盐浓度,最佳范围为0-3%。它在pH4至10之间表现出pH耐受性,在pH6.8-7.5时表现最佳。化学分类学,菌株IRAMC:0171T的特征在于二磷脂酰甘油,磷脂酰甘油,磷脂酰胆碱,和磷脂酰乙醇胺作为极性脂质。其主要脂肪酸组成为C18:1ω7c(61.2%),主要的泛醌是Q10(97%)。菌株IRAMC:0171T的16SrRNA基因的分析显示与WaimenseICMP19557T的中株根瘤菌具有99.08%的相似性,莫氏根瘤菌ACCC9665T,和华库中瘤菌IAM14158。然而,数字DNA-DNA杂交和平均核苷酸同一性分析显示值范围为21.1至25.2%和77.05至82.24%,分别,表明与既定的物种划界阈值存在重大偏差。系统发育研究,包含16SrRNA,基于全基因组的树重建,和核心蛋白质分析,定位菌株IRAMC:0171T最接近于中生根瘤菌KCTC72278T和\'中生根瘤菌\'UASWS1009T,在中根瘤菌属内形成一个独特的分支。考虑到这些全面的数据,我们建议菌株IRAMC:0171T(=DSM112841T=CECT30767T)作为新物种retamaesp的类型菌株。11月。
    A comprehensive polyphasic taxonomic investigation integrating taxongenomic criteria was conducted on strain IRAMC:0171T isolated from the root nodules of Retama raetam in Tunisia. This Gram-stain-negative and aerobic bacterium thrived within a temperature range of 5-45 °C, optimal at 28 °C, and tolerated salt concentrations from 0-6 % NaCl, with an optimal range of 0-3 %. It displayed pH tolerance from pH 4 to 10, thriving best at pH 6.8-7.5. Chemotaxonomically, strain IRAMC:0171T was characterized by diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, and phosphatidylethanolamine as polar lipids. Its predominant fatty acid composition was C18 : 1  ω7c (61.2 %), and the primary ubiquinone was Q10 (97 %). Analysis of the 16S rRNA gene of strain IRAMC:0171T showed 99.08 % similarity to Mesorhizobium waimense ICMP 19557T, Mesorhizobium amorphae ACCC 19665T, and Mesorhizobium huakuii IAM 14158. However, digital DNA-DNA hybridization and average nucleotide identity analyses revealed values ranging from 21.1 to 25.2 % and 77.05 to 82.24 %, respectively, signifying significant deviation from established species demarcation thresholds. Phylogenetic studies, encompassing 16S rRNA, whole-genome-based tree reconstruction, and core protein analysis, positioned strain IRAMC:0171T closest to Mesorhizobium terrae KCTC 72278T and \'Mesorhizobium hungaricum\' UASWS1009T, forming together a distinct branch within the genus Mesorhizobium. In consideration of this comprehensive data, we propose strain IRAMC:0171T (=DSM 112841T=CECT 30767T) as the type strain of a new species named Mesorhizobium retamae sp. nov.
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  • 文章类型: Journal Article
    一种新的革兰氏染色阴性,杆状,非孢子形成,有氧,具有单个极性或亚极性鞭毛的能动细菌,指定菌株H3510T,从烟台海岸收集的海洋藻类中分离出来,公关中国。生物体在28°C和pH7.0以及3.0%(w/v)NaCl存在下最佳生长。该菌株表现出过氧化氢酶活性阳性,但氧化酶和硝酸盐还原活性阴性。主要的细胞脂肪酸是C18:1ω7c和/或C18:1ω6c,11-甲基C18:1ω7c,C16:0此外,主要的极性脂质是磷脂酰甘油,磷脂酰甲基乙醇胺,二磷脂酰甘油,和磷脂酰乙醇胺;呼吸醌是泛醌10(Q-10)。H3510T菌株基因组DNAG+C含量为54.2%。该新菌株与多硅藻KMM9699T关系最密切,16SrRNA基因序列相似性为98.2%。H3510T菌株与系统发育相关的玫瑰属物种之间的平均核苷酸同一性和DNA-DNA杂交的计算值在71.3-74.9%和13.7-19.9%的范围内,分别。基于多相分析,菌株H3510T被鉴定为代表玫瑰属的一种新物种,为此,命名为红藻。11月。是提议的。菌株类型为H3510T(=KCTC8206T=MCCC1K04325T)。来自H3510T菌株的异源表达的肌醇2-脱氢酶基因对肌醇显示出高氧化活性,并显示出生产肌醇稀有立体异构体的潜力,如鲨肌醇。
    A novel Gram-stain-negative, rod-shaped, non-spore-forming, aerobic, motile bacterium with a single polar or subpolar flagellum, designated strain H3510T, was isolated from marine alga collected on sea shore of Yantai, PR China. The organism grew optimally at 28 °C and pH 7.0 and in presence of 3.0 % (w/v) NaCl. The strain exhibited positive catalase activity but negative oxidase and nitrate reduction activities. The predominant cellular fatty acids were C18 : 1  ω7c and/or C18 : 1  ω6c, 11-methyl C18 : 1  ω7c, and C16 : 0. Additionally, the major polar lipids were phosphatidylglycerol, phosphatidylmonomethylethanolamine, diphosphatidylglycerol, and phosphatidylethanolamine; the respiratory quinone was ubiquinone 10 (Q-10). The genomic DNA G+C content of strain H3510T was 54.2%. The novel strain showed the closest relationship with Roseibium polysiphoniae KMM 9699T with 98.2 % 16S rRNA gene sequence similarity. The calculated values for average nucleotide identity and DNA-DNA hybridization between strain H3510T and the phylogenetically related Roseibium species were in the range of 71.3-74.9 % and 13.7-19.9 %, respectively. Based on polyphasic analyses, strain H3510T was identified as representing a novel species of the genus Roseibium, for which the name Roseibium algae sp. nov. is proposed. The type strain is H3510T (=KCTC 8206T=MCCC 1K04325T). The heterologously expressed inositol 2-dehydrogenase gene from strain H3510T displayed high oxidation activity on myo-inositol and showed potential in the production of rare stereoisomers of inositol, such as scyllo-inositol.
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  • 文章类型: Journal Article
    使用多相分类学方法来表征这三种细菌菌株(FP830T,FP2034和FP2262)从水稻的根际土壤中分离,玉米,还有北京的高原大麦,黑龙江,西藏,分别,在PR中国。这些菌株是革兰氏阴性的,杆状,并有一个或两个极地鞭毛。在1%(w/v)NaCl存在下,它们在28°C和pH7.0下表现出最佳生长,并且在King'sB平板上培养时在紫外线下显示荧光。FP830T基因组大小为6.4Mbp,G+C含量为61.0mol%。FP830T具有通过产生各种代谢产物如fengycin来促进植物生长的潜力,pyoverdin,吲哚-3-乙酸,和挥发性物质2,3-丁二醇。系统发育分析表明,三个分离株形成了一个独立的分支,与菌株DSM13194T和zanjanenensisSWRI12T最密切相关。三个分离株和近亲之间的平均核苷酸同一性和数字DNA-DNA杂交值不高于93.7和52.3%,分别。主要的细胞脂肪酸是C16:0,总特征3(C16:1ω7c/C16:1ω6c),和求和特征8(C18:1ω7c/C18:1ω6c)。主要极性脂质是磷脂酰乙醇胺,二磷脂酰甘油,和氨基磷脂.主要的呼吸醌是泛醌(Q-9)。基于多相分类分析,结论是菌株FP830T,FP2034和FP2262代表假单胞菌属中的一个新物种,和北京假单胞菌。11月。被提议命名为新物种。菌株类型为FP830T(=ACCC62448T=JCM35689T)。
    A polyphasic taxonomic approach was used to characterize the three bacterial strains (FP830T, FP2034, and FP2262) isolated from the rhizosphere soil of rice, corn, and highland barley in Beijing, Heilongjiang, and Tibet, respectively, in PR China. These strains were Gram-negative, rod-shaped, and have one or two polar flagella. They exhibited optimal growth at 28 °C and pH 7.0 in the presence of 1 % (w/v) NaCl and showed fluorescence under ultraviolet light when cultivated on King\'s B plates. The FP830T genome size is 6.4 Mbp with a G+C content of 61.0 mol%. FP830T has the potential to promote plant growth by producing various metabolites such as fengycin, pyoverdin, indole-3-acetic acid, and the volatile substance 2,3-butanediol. Phylogenetic analysis indicated that three isolates formed an independent branch, which most closely related to type strains Pseudomonas thivervalensis DSM 13194T and Pseudomonas zanjanensis SWRI12T. The values of average nucleotide identity and digital DNA-DNA hybridization between three isolates and closest relatives were not higher than 93.7 and 52.3 %, respectively. The dominant cellular fatty acids were C16 : 0, summed feature 3 (C16 : 1  ω7c/C16 : 1 ω6c), and summed feature 8 (C18 : 1 ω7c/C18 : 1 ω6c). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, and aminophospholipid. The predominant respiratory quinone was ubiquinone (Q-9). Based on polyphasic taxonomic analysis, it was concluded that strains FP830T, FP2034, and FP2262 represented a novel species within the genus Pseudomonas, and Pseudomonas beijingensis sp. nov. was proposed for the name of novel species. The type strain is FP830T (=ACCC 62448T=JCM 35689T).
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  • 文章类型: Journal Article
    两个革兰氏染色阴性,杆状,不活动,需氧和产生类胡萝卜素的菌株,属于红杆菌科,指定为H149T和Z2T,从海南和浙江收集的滩涂沉积物样本中分离出来,中国公关,分别。菌株H149T的生长发生在15-42°C,0-10.0%(w/v)NaCl,和pH6.0-8.5,最佳温度为35-37°C,3.0-3.5%(w/v)NaCl和pH7.0。菌株Z2T在15-37°C生长,0-6.0%(w/v)NaCl,和pH6.0-9.5,最佳温度为25-30°C,0.5-1.0%(w/v)NaCl和pH6.0-6.5。泛醌-10是两个菌株中唯一的泛醌。H149T菌株的主要细胞脂肪酸为C16:0,求和特征3和求和特征8,而Z2T菌株的主要细胞脂肪酸为C17:1ω6c,求和特征3和求和特征8。菌株H149T和Z2T共享二磷脂酰甘油,磷脂酰甘油,磷脂酰乙醇胺,磷脂酰胆碱和鞘糖脂为主要极性脂质。16SrRNA基因序列同一性分析表明,H149T菌株与土木抗细菌KCTC23981T具有98.4%的最高序列同一性,菌株Z2T的含量为98.2%,QipengyuaniapacificaNZ-96T。基于16SrRNA基因和核心基因组序列的系统发育树显示,菌株H149T和Z2T在耳杆菌属和Qipengyuania属中形成了两个独立的进化枝,分别。菌株H149T的平均核苷酸同一性值为74.0-81.3%,与抗细菌型菌株的计算机DNA-DNA杂交值为18.5-23.1%,而Qipengyuania型菌株的Z2T值分别为73.3-78.7%和14.5-33.3%。H149T和Z2T菌株的基因组DNAGC含量分别为64.3和61.8%,分别。基于基因,基因组,系统发育,生理和化学分类学结果,菌株H149T(=KCTC8397T=MCCC1K08920T)和Z2T(=KCTC8396T=MCCC1K08946T)被认为代表了两种新的红杆菌科物种,其名称为Aurantiacibacter。11月。和浙江凤凤竹。11月。被提议,分别。
    Two Gram-stain-negative, rod-shaped, non-motile, aerobic and carotenoid-producing strains, belonging to the family Erythrobacteraceae, designated as H149T and Z2T, were isolated from tidal flat sediment samples collected in Hainan and Zhejiang, PR China, respectively. Growth of strain H149T occurred at 15-42 °C, 0-10.0 % (w/v) NaCl, and pH 6.0-8.5, with the optima at 35-37 °C, 3.0-3.5 % (w/v) NaCl and pH 7.0. Strain Z2T grew at 15-37 °C, 0-6.0 % (w/v) NaCl, and pH 6.0-9.5, with the optima at 25-30 °C, 0.5-1.0 % (w/v) NaCl and pH 6.0-6.5. Ubiquinone-10 was the sole ubiquinone in two strains. The predominant cellular fatty acids of strain H149T were C16 : 0, summed feature 3 and summed feature 8, while those of strain Z2T were C17 : 1  ω6c, summed feature 3 and summed feature 8. Strains H149T and Z2T shared diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and sphingoglycolipid as major polar lipids. The 16S rRNA gene sequence identity analysis indicated that strain H149T had the highest sequence identity of 98.4 % with Aurantiacibacter odishensis KCTC 23981T, and strain Z2T had that of 98.2 % with Qipengyuania pacifica NZ-96T. Phylogenetic trees based on 16S rRNA gene and core-genome sequences revealed that strains H149T and Z2T formed two independent clades in the genera Aurantiacibacter and Qipengyuania, respectively. Strain H149T had average nucleotide identity values of 74.0-81.3 % and in silico DNA-DNA hybridization values of 18.5-23.1 % with Aurantiacibacter type strains, while strain Z2T had values of 73.3-78.7 % and 14.5-33.3 % with Qipengyuania type strains. The genomic DNA G+C contents of strains H149T and Z2T were 64.3 and 61.8 %, respectively. Based on the genetic, genomic, phylogenetic, physiological and chemotaxonomic results, strains H149T (=KCTC 8397T=MCCC 1K08920T) and Z2T (=KCTC 8396T=MCCC 1K08946T) are concluded to represent two novel Erythrobacteraceae species for which the names Aurantiacibacter hainanensis sp. nov. and Qipengyuania zhejiangensis sp. nov. are proposed, respectively.
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  • 文章类型: Journal Article
    橙色色素,革兰氏染色阴性,严格的有氧,非鞭毛和杆状细菌,指定菌株DF17T,是从井子码头收集的沿海沉积物中分离出来的,威海,公关中国。确定最佳生长条件为30°C,pH7.5,并且在3%(w/v)NaCl中。根据16SrRNA基因序列的系统发育分析,DF17T菌株与WinogradskyellaaquimarisKCTC23502T的序列相似性最高,为96.9%。DNAG+C含量为35.8mol%,主要脂肪酸是iso-C15:1G,iso-C15:0和iso-C17:03-OH。主要极性脂质是两种氨基糖脂,一种磷脂酰乙醇胺和四种未鉴定的脂质。主要的呼吸醌是甲基萘醌-6(MK-6)。平均核苷酸同一性,数字DNA-DNA杂交,菌株DF17T与其他Winogradskyella物种之间的氨基酸同一性值低于69.35-72.95%的物种划分阈值,16.9-19.6%和71.25-78.93%,分别。根据其表型,遗传和生理特征,菌株DF17T被认为代表Winogradskyella属的一种新物种,名称Winogradskyellapelagiasp.11月。是提议的。菌株类型为DF17T(MCCC1H00456T=KCTC82421T)。
    An orange-pigmented, Gram-stain-negative, strictly aerobic, non-flagellated and rod-shaped bacterium, designated strain DF17T, was isolated from coastal sediment collected from Jingzi Wharf, Weihai, PR China. The optimal growth conditions were determined to be at 30 °C, pH 7.5, and in 3 % (w/v) NaCl. According to phylogenetic analysis of the 16S rRNA gene sequence, strain DF17T showed the highest sequence similarity of 96.9 % to Winogradskyella aquimaris KCTC 23502T. The DNA G+C content was 35.8 mol%, and the major fatty acids were iso-C15 : 1 G, iso-C15 : 0, and iso-C17 : 0 3-OH. The major polar lipids were two aminoglycolipids, one phosphatidylethanolamine and four unidentified lipids. The predominant respiratory quinone was menaquinone-6 (MK-6). The average nucleotide identity, digital DNA-DNA hybridization, and amino acid identity values between strain DF17T and other Winogradskyella species were below the species delineation thresholds of 69.35-72.95 %, 16.9-19.6 % and 71.25-78.93 %, respectively. On the basis of its phenotypic, genetic and physiological characteristics, strain DF17T is suggested to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella pelagia sp. nov. is proposed. The type strain is DF17T (MCCC 1H00456T=KCTC 82421T).
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