关键词: Sporothrix sp culture collections polyphasic taxonomy preservation sporotrichosis systematic review

Mesh : Sporothrix / classification isolation & purification genetics Sporotrichosis / microbiology Animals Humans Environmental Microbiology Preservation, Biological / methods

来  源:   DOI:10.3389/fcimb.2024.1382508   PDF(Pubmed)

Abstract:
UNASSIGNED: Sporotrichosis is a subcutaneous mycosis caused by fungi of the genus Sporothrix sp. Phenotypic and genotypic differences have been associated with their geographic distribution, virulence, or clinical manifestation of sporotrichosis. In the past decade, the interest in identifying species of the Sporothrix sp. has been increasing, due to its epidemiological importance and, in consequence, is important to know how to preserve them for future studies, in culture collection.
UNASSIGNED: The purposes of this study were to analyze the global distribution of environmental isolates and/or causal agents of sporotrichosis identified by polyphasic taxonomy, with mandatory use of molecular identification, and to evaluate the percentages and distribution of isolates stored in culture collections.
UNASSIGNED: A systematic review of articles on animal and human sporotrichosis and/or environmental isolation of the fungus, from 2007 to 2023, was done. Results: Our results demonstrated that, S. globosa, S. schenckii, and S. brasiliensis were the most identified species. With respect to the deposit and maintenance of species, we observed that only 17% of the strains of Sporothrix sp. isolated in the world are preserved in a culture collection.
UNASSIGNED: This systematic review confirmed a difficulty in obtaining the frequency of Sporothrix species stored in culture collection and insufficient data on the molecular identification mainly of animal sporotrichosis and isolation of Sporothrix sp. in environmental samples.
摘要:
孢子丝菌病是由孢子丝菌属真菌引起的皮下真菌病。表型和基因型差异与它们的地理分布有关,毒力,或孢子丝菌病的临床表现。在过去的十年里,对鉴定孢子丝菌物种的兴趣。一直在增加,由于其流行病学的重要性和,因此,知道如何保存它们以备将来研究很重要,文化收藏
本研究的目的是分析通过多相分类法鉴定的孢子丝菌病的环境分离物和/或致病因子的全球分布,强制使用分子鉴定,并评估培养物集合中储存的分离株的百分比和分布。
关于动物和人类孢子丝菌病和/或真菌环境隔离的文章的系统综述,从2007年到2023年,已经完成。结果:我们的研究结果表明,S、globosa,S.申克,巴西链球菌是鉴定最多的物种。关于物种的存放和维护,我们观察到只有17%的孢子丝菌。与世隔绝,保存在文化收藏中。
这项系统评价证实了难以获得培养物中存储的孢子丝菌物种的频率,并且有关主要是动物孢子丝菌病的分子鉴定和孢子丝菌的分离的数据不足。在环境样本中。
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