产生Musaspp的最有效方法。外植体涉及植物组织培养微繁殖技术的利用。然而,微生物污染的普遍挑战极大地阻碍了Musaspp的成功微繁殖。本研究研究了从柑橘果皮中获得的精油(LPO)和提取物(LPE)的抗氧化和抗菌特性。此外,我们探索了它们对抗Musaspp中常见微生物污染物的机制。微传播。使用气相色谱-质谱法,我们确定了LPO中的28个成分,与δ-柠檬烯,β-pine烯,柠檬醛,反式柠檬醛,β-双黑色素,乙酸香叶酯,和α-pine烯作为主要成分。同时,液相色谱-质谱联用技术检测LPE中17种成分,突出景天苷,橘皮素,scoparone,sinensetin,四甲基黄芩苷,5-去甲基金黄素,主要化合物为吡虫菊酯A。使用DPPH和ABTS方法进行的评估显示,LPE的IC50值为0.66±0.009和0.92±0.012mg/mL,分别,表明与LPO相比具有更高的抗氧化活性,使用相同的方法,IC50值为3.03±0.019和4.27±0.023mg/mL。通过体外测定,LPO和LPE均表现出对所有测试的污染微生物的抗微生物活性。采用时间杀伤分析的机械调查,细胞膜完整性的评估,和扫描电子显微镜(SEM)揭示了被测微生物污染物的形态特征的变化,随着LPO和LPE浓度和暴露时间的增加而加剧。这些改变导致了巨大的破坏,包括细胞壁裂解,细胞内成分的泄漏,以及随后的细胞死亡。因此,LPO和LPE是解决香蕉组织培养物中微生物污染的有希望的替代品。
The most effective methodologies for generating Musa spp. explants involve the utilization of plant tissue culture
micropropagation techniques. However, the pervasive challenge of microbial contamination significantly impedes the successful
micropropagation of Musa spp. This study examined the antioxidant and antibacterial characteristics of the essential oil (LPO) and extract (LPE) obtained from the peel of Citrus aurantifolia. Additionally, we explored their mechanisms against common microbial contaminants in Musa spp.
micropropagation. Using gas chromatography-mass spectrometry, we identified 28 components in LPO, with δ-limonene, β-pinene, citral, trans-citral, β-bisabolene, geranyl acetate, and α-pinene as the primary constituents. Meanwhile, liquid chromatography-mass spectrometry detected 17 components in LPE, highlighting nobiletin, tangeretin, scoparone, sinensetin, tetramethylscutellarein, 5-demethylnobiletin, and pyropheophorbide A as the predominant compounds. Evaluation using the DPPH and ABTS methods revealed the IC50 values for LPE at 0.66 ± 0.009 and 0.92 ± 0.012 mg/mL, respectively, indicating higher antioxidant activity compared to LPO, with IC50 values of 3.03 ± 0.019 and 4.27 ± 0.023 mg/mL using the same methods. Both LPO and LPE exhibited antimicrobial activities against all tested contaminant microorganisms through in vitro assays. Mechanistic investigations employing time-kill analysis, assessment of cell membrane integrity, and scanning electron microscopy (SEM) revealed changes in the morphological characteristics of the tested microbial contaminants, intensifying with increased concentration and exposure duration of LPO and LPE. These alterations led to substantial damage, including cell wall lysis, leakage of intracellular components, and subsequent cell death. Consequently, LPO and LPE emerge as promising alternatives for addressing microbial contamination in banana tissue cultures.