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Abstract：
This paper focuses on the creation of an in vitro collection of grapevine hybrids from the breeding program of the Kazakh Scientific Research Institute of Fruit Growing and Viticulture and investigates the presence of Plasmopara viticola resistance mediated by Rpv3 and Rpv12 loci. We looked at the optimization of in vitro establishment using either shoots taken directly from field-grown plants or from budwood cuttings forced indoors. We further screened for the presence of endophyte contamination in the initiated explants and optimized the multiplication stage. Finally, the presence of the resistance loci against P. viticola was studied. The shoots initiated from the field-sourced explants were the more effective method of providing plant sources for in vitro initiation once all plant accessions met the goal of in vitro establishment. The concentration of phytohormones and the acidity of the culture medium have a great effect on the multiplication rate and the quality of in vitro stock cultures. Out of 17 grapevine accessions, 16 showed the presence of single or combined resistance loci against P. viticola. The grapevine accessions identified as carrying Rpv3 and Rpv12 alleles represent important genetic resources for disease resistance breeding programs. These accessions may further contribute to the creation of new elite cultivars of economic interest.
摘要：
本文着重于从哈萨克斯坦水果种植和葡萄栽培科学研究所的育种计划中创建葡萄杂种的体外收集，并研究了由Rpv3和Rpv12基因座介导的Pasmoparaviticola抗性的存在。我们研究了使用直接从田间种植的植物或从室内强迫的芽中获取的芽来优化体外建立的方法。我们进一步筛选了起始外植体中内生菌污染的存在，并优化了繁殖阶段。最后,研究了针对蛇形疟原虫的抗性基因座的存在。一旦所有植物种质都达到了体外建立的目标，则从田间来源的外植体开始的芽是为体外启动提供植物来源的更有效方法。植物激素的浓度和培养基的酸度对体外培养的增殖速率和质量有很大影响。在17种小道消息中，图16显示了针对蛇形假单胞菌的单个或组合抗性基因座的存在。鉴定为携带Rpv3和Rpv12等位基因的葡萄种质代表了抗病育种计划的重要遗传资源。这些种质可能进一步有助于创造具有经济利益的新精英品种。
