PDF(Pubmed)
Abstract：
The endangered plant species Adenophora liliifolia faces threats to its survival in the wild, necessitating the development of effective micropropagation techniques for potential reintroduction efforts. This study demonstrates that Adenophora liliifolia effectively reproduces on MS synthetic medium with diverse plant growth regulators (PGR) and natural extracts, facilitating swift micropropagation for potential future reintroduction endeavors. It highlights the substantial impact of PGR composition and natural extracts on the growth and development of A. liliifolia. The ideal growth medium for A. liliifolia was determined to be ½ MS with specific treatments. Additionally, incorporating silver nitrate (AgNO3) at 5 mg L-1 into the medium led to enhanced root formation and shoot length, albeit excessive concentrations adversely affected root development. Varying concentrations of NAA significantly affected different plant growth parameters, with the 0.1 mg L-1 treatment yielding comparable plant height to the control. Moreover, 50 mL L-1 of coconut water bolstered root formation, while 200 mL L-1 increased shoot formation during in vitro propagation. However, elevated doses of coconut water (CW) impeded root development but stimulated shoot growth. Experiments measuring chlorophyll a + b and carotenoid content indicated higher concentrations in the control group than differing levels of applied coconut water. Optimizing pH levels from 6.8-7 to 7.8-8.0 notably enhanced plant height and root formation, with significant carotenoid accumulation observed at pH 6.8-7. Soil samples from A. liliifolia\'s natural habitat exhibited a pH of 6.65. Ultimately, the refined in vitro propagation protocol effectively propagated A. liliifolia, representing a pioneering effort and setting the stage for future restoration initiatives and conservation endeavors.
摘要：
濒临灭绝的植物物种雪莲在野外生存面临威胁，有必要开发有效的微繁殖技术以进行潜在的重新引入。这项研究表明，雪莲在MS合成培养基上有效繁殖，具有多种植物生长调节剂（PGR）和天然提取物，促进快速微传播，以促进未来潜在的重新引入努力。它强调了PGR组合物和天然提取物对A的生长和发育的实质性影响。在特定处理下,确定百合A的理想生长培养基为1/2MS。此外,将5mgL-1的硝酸银（AgNO3）掺入培养基中导致根形成和芽长度增加，尽管浓度过高对根系发育产生不利影响。不同浓度的NAA显著影响不同的植物生长参数,0.1mgL-1处理产生与对照相当的植物高度。此外，50mLL-1的椰子水支持根形成，而200mLL-1在体外繁殖过程中增加了芽的形成。然而,高剂量的椰子水（CW）阻碍了根的发育，但刺激了芽的生长。测量叶绿素ab和类胡萝卜素含量的实验表明，对照组的浓度高于不同水平的椰子水。将pH值从6.8-7优化到7.8-8.0，显着增强了植物高度和根系形成，在pH6.8-7时观察到明显的类胡萝卜素积累。来自A.liliifolia的自然栖息地的土壤样品的pH值为6.65。最终,完善的体外繁殖方案有效繁殖了A.Liliifolia，代表了一项开创性的努力，并为未来的恢复举措和保护工作奠定了基础。
