本研究是在组织培养实验室进行的,园艺部门,农业学院,Damietta大学,埃及。这项研究的目的是建立适合三种进口桃砧木的微繁殖协议:冲绳(P.persica),Nemared(P.persica×P.davidiana)×P.persica),和Garnem(P.体外dulcis×P.persica)。结果表明,将外植体以20%的次氯酸钠(NaOCl)浸泡15分钟可产生最高的响应性(82.81%),生存率(96.61%),死亡率最低(3.14%),污染最低(0.24%)。Garnem基因型外植体反应最好(89.12%),生存率(90.62%),最低死亡率(0.00%),与其他基因型相比,污染最高(9.37%)。与腋芽相比,射击提示显示出最高的响应能力,生存,和死亡(100、87.40和12.59%,分别),以及最不显著的污染(0.00%)。此外,响应的百分比,幸存下来,死了,在不同的收集日期,受污染的外植体差异很大。使用的6-苄基氨基嘌呤(BAP)浓度(3至5.0mg/L)在体外增殖方面表现出相似的行为,冲绳每个外植体的生长率为3.77至6.11,4.33至8.88和3.33至7.44,Nemared,和Garnem桃子砧木,分别,表明芽增殖的数量是基因型依赖性的。此外,使用5.0mg/LBAP与0.2mg/LIBA的组合显着增加平均芽增殖(96.29%),每个外植体的枝条数(7.48),与其他处理相比,平均叶片数/外植体(16.33)。基于这些结果,在冲绳的体外繁殖过程中,不定芽的发育得到了增强,Nemared,和Garnem桃砧木通过吲哚-丁酸(IBA)和6-苄基氨基嘌呤(BAP)的协同作用。
The present
study was conducted in the Laboratory of Tissue Culture, Horticulture Department, Faculty of Agriculture, Damietta University, Egypt. The objective of this
study was to establish a
micropropagation protocol suitable for three imported peach rootstocks: Okinawa (P. persica), Nemared (P. persica × P. davidiana) × P. persica), and Garnem (P. dulcis × P. persica) in vitro. The results showed that soaking the explants in sodium hypochlorite (NaOCl) at 20% for 15 min produced the highest responsiveness (82.81%), survival (96.61%), with the lowest mortality (3.14%) and contamination (0.24%). Explants of the Garnem genotype had the best response (89.12%), survival (90.62%), lowest mortality (0.00%), and highest contamination (9.37%) when compared to the other genotypes. In comparison with axillary buds, the shoot tip displayed the highest responsiveness, survival, and death (100, 87.40, and 12.59%, respectively), as well as the least significant contamination (0.00%). Additionally, the percentages of responsive, survived, dead, and contaminated explants at the various collection dates varied significantly. The 6-benzylaminopurine (BAP) concentrations used (3 to 5.0 mg/L) demonstrated similar behavior in terms of in vitro proliferation, with rates of 3.77 to 6.11, 4.33 to 8.88, and 3.33 to 7.44 shoot numbers per explant for the Okinawa, Nemared, and Garnem peach rootstocks, respectively, indicating that the number of shoot proliferations is genotype-dependent. Additionally, using 5.0 mg/L BAP in combination with 0.2 mg/L IBA significantly increased average shoot proliferation (96.29%), number of shoots per explant (7.48), and average leaf number/explant (16.33) compared to the other treatments. Based on these results, adventitious bud development was enhanced during in vitro multiplication of the Okinawa, Nemared, and Garnem peach rootstocks by the synergistic interaction of indole-butyric acid (IBA) and 6-benzylaminopurine (BAP).