■系统性红斑狼疮(SLE)是一种慢性,自身免疫性疾病,通常以严重的病程和不清楚的病因为特征。蛋白质糖基化反应,也被称为糖化,可能与SLE的病因有关。晚期糖基化终产物(AGEs)表现出细胞毒性,影响细胞信号,削弱细胞外蛋白质的功能,并可能充当新表位。葡萄糖酮(GS),乙二醛(GO),和甲基乙二醛(MGO)是参与糖氧化的α-二羰基化合物(α-DC)的实例。该研究旨在评估这三种化合物在SLE患者血清中的浓度,并将结果与健康个体进行比较。
■31名患有SLE的妇女和26名健康个体被纳入研究。采用荧光检测的高效液相色谱法评估血清样品中α-DC的浓度。结果与疾病持续时间等参数之间的相关性,年龄,肾小球滤过率(GFR),系统性红斑狼疮疾病活动指数2000(SLEDAI-2K),和肌酐进行分析。
■SLE患者表现出较低浓度的葡萄糖酮,乙二醛,和甲基乙二醛比对照组。相关性分析显示被检查组之间存在差异。
■在患有SLE的女性中,α-DCs代谢过程发生了改变。SLE患者的特征在于低血清水平的α-DC。我们假设,在氧化条件下,蛋白酶体降解受阻或α-DC的快速消耗可能会导致观察到的这些化合物的低浓度。
UNASSIGNED: Systemic lupus erythematosus (SLE) is a chronic, autoimmune disease, often characterised by severe course and unclear etiopathogenesis. The reaction of protein glycoxidation, also known as glycation, may be linked to etiopathogenesis of SLE. Advanced glycation end-products (AGEs) exhibit cytotoxic properties, affect cellular signalling, impair functions of extracellular proteins, and may act as neoepitopes. Glucosone (GS), glyoxal (GO), and
methylglyoxal (MGO) are examples of α-dicarbonyl compounds (α-DCs) partaking in glycoxidation. The study aimed to evaluate concentrations of these three compounds in blood serum of SLE patients, and to compare the results with healthy individuals.
UNASSIGNED: 31 women suffering from SLE and 26 healthy individuals were included in the study. High-performance liquid chromatography with fluorescence detection was applied to evaluate concentrations of α-DCs in their serum samples. Correlations between the results and parameters such as disease duration time, age, glomerular filtration rate (GFR), Systemic Lupus Erythematosus Disease Activity Index 2000 (SLEDAI-2K), and creatinine were analysed.
UNASSIGNED: The SLE patients exhibited lower concentrations of glucosone, glyoxal, and
methylglyoxal than the control group. Analysis of correlations showed a difference between the examined groups.
UNASSIGNED: In women suffering from SLE the course of α-DCs metabolism is altered. SLE patients are characterised by low serum levels of α-DCs. We hypothesise that either hindered proteasomal degradation or fast consumption of α-DCs in oxidative conditions may cause the observed low concentration of these compounds.