二羰基化合物是高度反应性化合物和高级糖基化终产物(AGEs)的主要前体。二羰基和AGEs都与年龄相关疾病的发展有关。二羰基是内源性形成的,也是在食品加工过程中形成的。饮食中的二羰基在多大程度上有助于组织中循环的二羰基和AGEs是未知的。
研究膳食二羰基摄入量与血浆二羰基浓度和皮肤AGEs的横断面关系。
在基于人口的马斯特里赫特研究的2566个人中(年龄:60±8岁,50%的男性,26%患有2型糖尿病),我们估计习惯性摄入二羰基甲基乙二醛(MGO),乙二醛(GO),和3-脱氧葡萄糖酮(3-DG)通过结合FFQs与我们的饮食二羰基MGO数据库,GO,和>200种常用食品中的3-DG浓度。MGO的空腹血浆浓度,GO,采用超高效液相色谱-串联质谱法测定3-DG。皮肤AGEs测量为皮肤自发荧光(SAF),使用年龄阅读器。使用线性回归模型检查了膳食二羰基摄入量与各自的血浆浓度和SAF(全部标准化)的关联。根据年龄调整,性别,与心脏代谢危险因素相关的潜在混杂因素,和生活方式。
MGO的摄入中位数,GO,3-DG分别为3.6、3.5和17mg/d,分别。咖啡是MGO的主要饮食来源,而这是GO和3-DG的面包。在完全调整的模型中,饮食MGO与血浆MGO(β:0.08;95%CI:0.02,0.13)和SAF(β:0.12;95%CI:0.07,0.17)相关。膳食GO与血浆GO相关(β:0.10;95%CI:0.04,0.16),但与SAF无关。3-DG与血浆3-DG或SAF均无显著相关性。
饮食中MGO和GO的习惯性摄入量较高,但不是3-DG,与较高的相应血浆浓度有关。更高的MGO摄入量也与更高的SAF相关。这些结果表明MGO和GO的饮食吸收。需要确定MGO和GO的饮食吸收的生物学意义。该研究已获得机构医学伦理委员会(NL31329.068.10)和卫生部长的批准,荷兰的福利和体育(许可证131088-105234-PG)。
Dicarbonyls are highly reactive compounds and major precursors of advanced glycation end products (AGEs). Both dicarbonyls and AGEs are associated with development of age-related diseases. Dicarbonyls are formed endogenously but also during food processing. To what extent dicarbonyls from the diet contribute to circulating dicarbonyls and AGEs in tissues is unknown.
To examine cross-sectional associations of dietary dicarbonyl intake with plasma dicarbonyl concentrations and skin AGEs.
In 2566 individuals of the population-based Maastricht
Study (age: 60 ± 8 y, 50% males, 26% with type 2 diabetes), we estimated habitual intake of the dicarbonyls
methylglyoxal (MGO), glyoxal (GO), and 3-deoxyglucosone (3-DG) by combining FFQs with our dietary dicarbonyl database of MGO, GO, and 3-DG concentrations in > 200 commonly consumed food products. Fasting plasma concentrations of MGO, GO, and 3-DG were measured by ultra-performance liquid chromatography-tandem mass spectrometry. Skin AGEs were measured as skin autofluorescence (SAF), using the AGE Reader. Associations of dietary dicarbonyl intake with their respective plasma concentrations and SAF (all standardized) were examined using linear regression models, adjusted for age, sex, potential confounders related to cardiometabolic risk factors, and lifestyle.
Median intake of MGO, GO, and 3-DG was 3.6, 3.5, and 17 mg/d, respectively. Coffee was the main dietary source of MGO, whereas this was bread for GO and 3-DG. In the fully adjusted models, dietary MGO was associated with plasma MGO (β: 0.08; 95% CI: 0.02, 0.13) and SAF (β: 0.12; 95% CI: 0.07, 0.17). Dietary GO was associated with plasma GO (β: 0.10; 95% CI: 0.04, 0.16) but not with SAF. 3-DG was not significantly associated with either plasma 3-DG or SAF.
Higher habitual intake of dietary MGO and GO, but not 3-DG, was associated with higher corresponding plasma concentrations. Higher intake of MGO was also associated with higher SAF. These results suggest dietary absorption of MGO and GO. Biological implications of dietary absorption of MGO and GO need to be determined. The
study has been approved by the institutional medical ethical committee (NL31329.068.10) and the Minister of Health, Welfare and Sports of the Netherlands (Permit 131088-105234-PG).