在本文中,建立了一种新的模拟酶联免疫检测方法(BELISA),基于催化活性氧化铜@金纳米颗粒(CuO@AuNPs)作为标记和分子印迹聚合物(MIP)作为仿生抗体。在优化条件下,BELISA方法对组胺和色胺的检测限制分别为0.04mgL-1和0.14mgL-1。对于掺有组胺和色胺的酒,BELISA方法的回收率在89.90%至115.00%之间。此外,鱼中组胺和色胺的含量,酱油,用BELISA法和高效液相色谱法检测米醋样品,两种方法之间没有显着差异。虽然纳米酶的催化活性仍然低于天然酶,BELISA方法仍然可以灵敏地确定食物样品中的组胺和色胺水平。
In this paper, a novel biomimetic enzyme-linked immunoassay method (BELISA) was successfully established for the detection of histamine and
tryptamine, based on catalytically active cupric oxide@gold nanoparticles (CuO@Au NPs) as a marker and a molecularly imprinted polymer (MIP) as the biomimetic antibody. Under optimized conditions, the detection limitations of the BELISA method for histamine and
tryptamine were 0.04 mg L-1 and 0.14 mg L-1, respectively. For liquor spiked with histamine and
tryptamine, the BELISA method delivered satisfactory recoveries ranging from 89.90% to 115.00%. Furthermore, the levels of histamine and
tryptamine in fish, soy sauce, and rice vinegar samples were detected by the BELISA method and a high performance liquid chromatography method, with no significant difference between the two methods being found. Although the catalytic activity of nanozymes is still lower than that of natural enzymes, the BELISA method could still sensitively determine the histamine and
tryptamine levels in food samples.