Abstract：
Rapid and accurate quantification of trace targets in complex samples is an extremely challenging issue in fast analysis field. Herein, we developed Fe3O4-carboxyl modified AuNPs-chitosan@AgNPs composite (Fe3O4-AuNCs-Cs@AgNPs) as a robust surface-enhanced Raman scattering (SERS) substrate for rapid analysis of tryptamine (TPA) and ofloxacin (OFX). The substrate possessed abundant surficial active sites of -NH2, -OH and -COOH groups. The substrate exhibited good SERS activity for several different model molecules with enhancement factors (EFs) of 1.2 × 108 for 4-mercaptobenzoic acid. The substrate presented good stability for detection of TPA at pH 6.0 and OFX at pH 8.0, and relative standard deviations less than 5.0% for intra-batch and 6.0% for inter-batch. Also, the substrate possessed good time-stability within 50 days. The substrate integrated advantages of efficient enrichment, fast magnetic separation, and strong localized surface plasmon resonance properties of AgNPs. With versatile merits, TPA and OFX can be enriched and separated within 10 min. SERS methods for analysis of TPA and OFX were developed with detection limits of 35.5 μg/L and 15.8 μg/L, respectively. TPA and OFX were actually found in aquatic product, and recoveries during sample analysis were 89.3%-110% for TPA and 89.3%-96.8% for OFX. The analytical process completed within 30 min via enrichment-separation-detection all-in-one, exhibiting great potential for rapid analysis of toxic biogenic monoamines and antibiotic residues in food.
摘要：
在快速分析领域，复杂样品中痕量目标的快速准确定量是一个极具挑战性的问题。在这里，我们开发了Fe3O4-羧基修饰的AuNPs-壳聚糖@AgNPs复合材料（Fe3O4-AuNCs-Cs@AgNPs）作为强大的表面增强拉曼散射（SERS）基材，用于快速分析色胺（TPA）和氧氟沙星（OFX）。底物具有丰富的-NH2，-OH和-COOH基团的表面活性位点。底物对几种不同的模型分子表现出良好的SERS活性，对4-巯基苯甲酸的增强因子（EF）为1.2×108。底物在pH6.0下检测TPA和pH8.0下检测OFX具有良好的稳定性，批内相对标准偏差小于5.0％，批间相对标准偏差为6.0％。此外，底物在50天内具有良好的时间稳定性。高效富集的底物综合优势，快速磁选，和AgNPs的强局域表面等离子体共振特性。具有多才多艺的优点，TPA和OFX可以在10分钟内富集和分离。建立了用于分析TPA和OFX的SERS方法，检出限为35.5μg/L和15.8μg/L。分别。TPA和OFX实际上是在水产品中发现的，在样品分析中，TPA的回收率为89.3％-110％，OFX的回收率为89.3％-96.8％。分析过程在30分钟内通过富集-分离-检测一体化完成，显示出快速分析食品中有毒生物单胺和抗生素残留的巨大潜力。
