背景:挥发性有机化合物(VOC)是主要的室内空气污染物。先前的研究报道了VOC暴露与过敏性疾病之间的关联。这里,我们旨在探讨VOC暴露与成人特应性皮炎(AD)之间的关系。
方法:我们前瞻性招募了31名成年AD患者和11名健康受试者作为对照。尿代谢产物VOCs水平,包括1.3-丁二烯,丙烯酰胺,苯,甲苯,和二甲苯,均采用液相色谱-质谱法测定。使用针对年龄和性别进行调整的多元线性回归模型检查了AD与经对数转化的尿液中VOC代谢物水平之间的关系。我们还用1,3-丁二烯和甲苯处理了小鼠骨髓来源的细胞(BMMC),并测量了β-己糖胺酶的释放。
结果:我们的结果表明,肌酐校正的尿中N-乙酰基-S-(3,4-二羟丁基)-L-半胱氨酸(DHBMA),N-乙酰基-S-(2-氨基甲酰基-2-羟乙基)-L-半胱氨酸(GAMA),与对照组相比,AD患者的N-乙酰-S-(苄基)-L-半胱氨酸(BMA)均升高。在多元线性回归模型中,BMA(甲苯代谢物)和DHBMA(1,3-丁二烯代谢物)的肌酐校正尿水平在AD患者中出现升高,尽管经多重比较校正后未达到统计学意义.此外,1,3-丁二烯和甲苯可以刺激BMMC脱颗粒多达化合物48/80。
结论:一些挥发性有机化合物,如1,3-丁二烯和甲苯,可能与成人AD发病有关。
BACKGROUND: Volatile organic compounds (VOC) are major indoor air pollutants. Previous studies reported an association between VOC exposure and allergic diseases. Here, we aimed to explore the relationship between VOC exposure and atopic dermatitis (AD) in adults.
METHODS: We prospectively enrolled 31 adult AD patients and 11 healthy subjects as controls. Urine metabolite levels of VOCs, including 1.3-butadiene, acrylamide, benzene,
toluene, and xylene, were all determined with liquid chromatography-mass spectrometry. The relationship between AD and log-transformed urine levels of VOC metabolites were examined using a multivariate linear regression model adjusted for age and sex. We also treated mouse bone marrow-derived cells (BMMCs) with 1,3-butadiene and
toluene and measured the release of β-hexosaminidase.
RESULTS: Our results demonstrated that creatinine-corrected urine levels of N-Acetyl-S- (3,4-dihydroxybutyl)-L-cysteine (DHBMA), N-Acetyl-S-(2-carbamoyl-2-hydroxyethyl)-L-cysteine (GAMA), and N-Acetyl-S-(benzyl)-L-cysteine (BMA) were all elevated in AD patients compared with controls. In a multivariate linear regression model, creatinine-corrected urine levels of BMA (a
toluene metabolite) and DHBMA (a 1,3-butadiene metabolite) appeared elevated in AD patients, although statistical significance was not reached after correction for multiple comparisons. In addition, 1,3-butadiene and
toluene could stimulate BMMCs to degranulate as much as compound 48/80.
CONCLUSIONS: Some VOCs, such as 1,3-butadiene and
toluene, might be associated with AD pathogenesis in adults.