Abstract：
Natural attenuation (NA) is of great significance for the remediation of contaminated groundwater, and how to identify NA patterns of toluene in aquifers more quickly and effectively poses an urgent challenge. In this study, the NA of toluene in two typical soils was conducted by means of soil column experiment. Based on column experiments, dissolved organic matter (DOM) was rapidly identified using fluorescence spectroscopy, and the relationship between DOM and the NA of toluene was established through structural equation modeling analysis. The adsorption rates of toluene in clay and sandy soil were 39 % and 26 %, respectively. The adsorption capacity and total NA capacity of silty clay were large. The occurrence of fluorescence peaks of protein-like components and specific products indicated the occurrence of biodegradation. Arenimonas, Acidovorax and Brevundimonas were the main degrading bacteria identified in Column A, while Pseudomonas, Azotobacter and Mycobacterium were the main ones identified in Column B. The pH, ORP, and Fe(II) were the most important factors affecting the composition of microbial communities, which in turn affected the NA of toluene. These results provide a new way to quickly identify NA of toluene.
摘要：
自然衰减(NA)对污染地下水的修复具有重要意义,以及如何更快速有效地识别含水层中甲苯的NA模式提出了紧迫的挑战。在这项研究中,通过土柱试验对两种典型土壤中甲苯的NA进行了测定。根据色谱柱实验，使用荧光光谱法快速鉴定溶解有机物(DOM)，通过结构方程模型分析,建立了DOM与甲苯NA的关系。甲苯在粘土和沙质土壤中的吸附率分别为39%和26%,分别。粉质粘土的吸附容量和总NA容量较大。蛋白质样成分和特定产物的荧光峰的出现表明了生物降解的发生。Arenimonas,Acidovorax和Brevundimonas是A列中鉴定的主要降解菌，而假单胞菌,在B列中鉴定的主要是固氮菌和分枝杆菌。ORP,和Fe(II)是影响微生物群落组成的最重要因素，进而影响甲苯的NA。这些成果供给了一种疾速辨认甲苯NA的新办法。
