在许多职业环境中,工人经常接触甲苯和噪音。然而,这些暴露对心血管系统的个体和综合影响尚未完全阐明。因此,这项研究旨在研究同时暴露于甲苯和噪声对大鼠心脏的影响,同时还评估了橄榄叶提取物(OLE)的潜在预防作用。将48只雄性Wistar大鼠随机分为8组(n=6只/组):对照组(C),接受OLE(C+OLE)的对照组,暴露于噪声的组(N),暴露于噪声并接受OLE(N+OLE)的组,暴露于甲苯的组(T),暴露于甲苯并接受OLE(T+OLE)的组,共同暴露于噪声和甲苯(NT)的组,和共同暴露于噪声和甲苯并接受OLE(NT+OLE)的组。这项研究中的大鼠同时暴露于甲苯和噪音中,持续六周,在一个定制的有机玻璃室。甲苯的浓度为300ppm,而噪声水平设置为85dB(A)。暴露室装有发电系统,曝光系统,和一个监控系统,确保精确和准确的曝光条件。六周后,从大鼠中收集心脏和血液样品用于后续分析。血浆胆固醇水平(CHOL),甘油三酯(TG),乳酸脱氢酶(LDH),和肌酸激酶(CK)进行测量,并采用HE染色进行组织病理学检查。此外,超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性,测量心脏组织中的丙二醛(MDA)水平。我们的结果表明,同时暴露于噪声和甲苯会改变CHOL,TG,LDH,和CK水平,并导致脂质过氧化水平和超氧化物歧化酶活性增加,随着心脏过氧化氢酶活性的降低。还观察到心肌的显著改变。然而,发现用OLE治疗可以调节这些氧化和组织学变化,最终纠正噪声和甲苯联合暴露引起的有害影响。因此,我们的研究表明,OLE可能是在工业环境中暴露于甲苯和噪音的个人的潜在预防措施。
In many occupational settings, workers are frequently exposed to toluene and noise. However, the individual and combined effects of these exposures on the cardiovascular system have not been fully elucidated. Therefore, this
study aimed to investigate the impact of simultaneous exposure to
toluene and noise on the rat heart, while also evaluating the potential preventive effect of olive leaf extract (OLE). Forty-eight male Wistar rats were randomly assigned to eight groups (n = 6/group): control group (C), control group that received OLE (C + OLE), group exposed to noise (N), group exposed to noise and receiving OLE (N + OLE), group exposed to toluene (T), group exposed to
toluene and receiving OLE (T + OLE), group co-exposed to noise and
toluene (NT), and group co-exposed to noise and toluene and receiving OLE (NT + OLE). The rats in this
study were subjected to simultaneous exposure to
toluene and noise for a duration of six weeks, within a custom-built plexiglass chamber. Toluene was administered at a concentration of 300 ppm, while the noise level was set to 85 dB(A). The exposure chamber was equipped with a generation system, an exposure system, and a monitoring system, ensuring precise and accurate exposure conditions. After the six-week period, heart and blood samples were collected from the rats for subsequent analysis. Plasma levels of cholesterol (CHOL), triglycerides (TG), lactate dehydrogenase (LDH), and creatine kinase (CK) were measured, and histopathological investigation was conducted using HE staining. Additionally, superoxide dismutase (SOD) and catalase (CAT) activities, as well as malondialdehyde (MDA) levels in heart tissue were measured. Our results showed that simultaneous exposure to noise and toluene altered CHOL, TG, LDH, and CK levels, and also caused an increase in lipid peroxidation levels and superoxide dismutase activity, along with a decrease in catalase activity in the heart. A significant alteration in the myocardium was also observed. However, treatment with OLE was found to modulate these oxidative and histological changes, ultimately correcting the deleterious effects induced by the combined exposure to noise and toluene. Therefore, our
study suggests that OLE could be a potential preventive measure for individuals exposed to
toluene and noise in industrial settings.