hair cycle

头发周期
  • 文章类型: Journal Article
    UNASSIGNED: To summarize the dynamic and synchronized changes between the hair cycle and dermal adipose tissue as well as the impact of dermal adipose tissue on hair growth, and to provide a new research idea for the clinical treatment of hair loss.
    UNASSIGNED: An extensive review of relevant literature both domestic and international was conducted, analyzing and summarizing the impact of dermal adipose precursor cells, mature dermal adipocytes, and the processes of adipogenesis in dermal adipose tissue on the transition of hair cycle phases.
    UNASSIGNED: Dermal adipose tissue is anatomically adjacent to hair follicles and closely related to the changes in the hair cycle. The proliferation and differentiation of dermal adipose precursor cells promote the transition of hair cycle from telogen to anagen, while mature adipocytes can accelerate the transition from anagen to catagen of the hair cycle by expressing signaling molecules, with adipogenesis in dermal adipose tissue and hair cycle transition signaling coexistence.
    UNASSIGNED: Dermal adipose tissue affects the transition of the hair cycle and regulates hair growth by secreting various signaling molecules. However, the quantity and depth of existing literature are far from sufficient to fully elucidate its prominent role in regulating the hair cycle, and the specific regulatory mechanisms needs to be further studied.
    UNASSIGNED: 总结毛发周期和真皮脂肪之间的动态同步变化以及真皮脂肪对毛发生长的影响,为临床治疗脱发提供新的研究思路。.
    UNASSIGNED: 广泛查阅国内外相关文献,分析和总结真皮脂肪前体细胞、真皮成熟脂肪细胞以及成脂分化过程对毛发周期转变的影响。.
    UNASSIGNED: 真皮脂肪组织与毛囊解剖结构相邻,并且与毛发周期变化息息相关。真皮脂肪前体细胞的增殖分化促进毛发由静止期向生长期转变,但成熟脂肪细胞能够通过表达调节毛发周期的信号分子以加速毛发周期生长期-退行期转变。真皮脂肪的成脂分化与毛发周期转变共同受到多种信号通路的综合调节。.
    UNASSIGNED: 真皮脂肪通过分泌多种信号分子来影响毛发周期转变以调节毛发生长,但目前研究结果尚不足以充分阐明真皮脂肪在调节毛发周期中的突出作用,具体调节机制有待进一步研究。.
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  • 文章类型: Journal Article
    现有研究将氧化应激和炎症与脱发联系起来。丹酚酸B(SAB)以其抗氧化作用而闻名,抗炎,和其他有益的药理特性。
    评估SAB在调节毛发生长中的功效。
    使用C57BL/6小鼠进行体内实验,以评估SAB对头发和皮肤参数的影响。该研究涉及人毛囊(HF)的离体分析,用于毛干长度和毛发生长周期评估。体外,人毛乳头细胞(hDPC)用SAB培养,以及它们的扩散,防止H2O2诱导的氧化损伤,使用各种分析技术检查基因/蛋白质表达改变,包括实时细胞分析(RTCA),DCFH-DA测定,RNA-seq,和KEGG通路分析。
    小鼠中的SAB治疗到第21天显着改善了毛发生长和血管形成。在人类HFs中,SAB延长了毛干长度,并延迟了向前阶段的过渡。SAB处理的hDPC显示氧化-抗氧化相关基因和蛋白质的表达显着降低,包括ERK和p38的磷酸化水平降低。
    研究表明,SAB促进hDPC增殖并提供抗氧化应激保护,突出了其作为增强头发生长和治疗脱发的治疗剂的潜力。
    UNASSIGNED: Existing research links oxidative stress and inflammation to hair loss. Salvianolic acid B (SAB) is known for its anti-oxidative, anti-inflammatory, and other beneficial pharmacological properties.
    UNASSIGNED: To assess the efficacy of SAB in modulating hair growth.
    UNASSIGNED: In vivo experiments were conducted using C57BL/6 mice to evaluate the effects of SAB on hair and skin parameters. The study involved ex vivo analysis of human hair follicles (HFs) for hair shaft length and hair growth cycle assessment. In vitro, human dermal papilla cells (hDPCs) were cultured with SAB, and their proliferation, protection against H2O2-induced oxidative damage, and gene/protein expression alterations were examined using various analytical techniques, including Real-Time Cell Analysis (RTCA), DCFH-DA Assay, RNA-seq, and KEGG pathway analysis.
    UNASSIGNED: SAB treatment in mice significantly improved hair growth and vascularization by day 21. In human HFs, SAB extended hair shaft length and delayed the transition to the catagen phase. SAB-treated hDPCs showed a notable decrease in the expression of oxidation-antioxidation-related genes and proteins, including reduced phosphorylation levels of ERK and p38.
    UNASSIGNED: The study indicates that SAB promotes hDPC proliferation and offers protection against oxidative stress, highlighting its potential as a therapeutic agent for enhancing hair growth and treating hair loss.
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  • 文章类型: Journal Article
    甲状腺素对于维持身体的正常运转很重要。临床和实验结果均显示甲状腺素与毛发生长密切相关,其机制尚未完全理解。
    研究甲状腺素受体激动剂的作用,TDM10842,用于C3H小鼠背毛生长并探讨其潜在机制。
    将脱毛小鼠用TDM10842、该药物的媒介物施用,并且在背部皮肤上没有任何材料。采用RNA测序(RNA-seq)来鉴定皮肤组织的基因表达的变化。进行定量实时PCR(rt-PCR)和免疫印迹以验证不同组之间的关键差异表达基因(DEGs)。
    TDM组显示早期诱导生长期。在3组之间鉴定了857、782和276个差异表达的基因。作为组TDM中的关键DEG,Pclaf与Wnt/β-catenin和Hedgehog信号通路的动机呈正相关,具有高表达的Ki67和cyclinD1。
    TDM10842加速了生长期的进入,潜在的机制可能是Wnt/β-catenin和Hedgehog途径的激活。Pclaf是参与通路激活的关键分子,cyclinD1是该途径下游的重要效应蛋白。
    UNASSIGNED: Thyroxine is important to maintain the normal operation of the body. Both clinical and experimental results show thyroxine is closely related to hair growth, the mechanism of which is not fully understood.
    UNASSIGNED: Investigate the effect of thyroxine receptor agonist, TDM10842, for dorsal hair growth in C3H mice and explore its underlying mechanism.
    UNASSIGNED: Depilated mice were applied with the TDM10842, vehicle of this drug and without any materials on dorsal skin. RNA-sequencing (RNA-seq) was employed to identify the change in gene expression of skin tissues. Quantitative real-time PCR (rt-PCR) and immunoblotting were conducted to validate key differentially expressed genes (DEGs) between different groups.
    UNASSIGNED: The TDM group showed early induction of anagen. 857, 782, and 276 differentially expressed genes were identified between 3 groups. As a critical DEG in group TDM, Pclaf was positively related to the motivation of Wnt/beta-catenin and Hedgehog signaling pathways, with a high expression of Ki67 and cyclinD1.
    UNASSIGNED: TDM10842 accelerates the anagen entrance and the potential mechanism might be the activation of Wnt/beta-catenin and Hedgehog pathways. Pclaf serves as a critical molecule involved in pathway activation, and cyclinD1 is an important effector protein downstream of the pathways.
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  • 文章类型: Journal Article
    适应高原环境的极端条件,牦牛进化出浓密的头发,使它们成为研究头发生长机制的理想模型。通过研究牦牛,我们可以获得有关毛囊在充满挑战的环境中如何发育及其周期性生长的宝贵见解。然而,缺乏关于牦牛毛囊组织学的基本数据,以及缺乏毛囊的体外细胞模型,这限制了这些研究目标。在这项研究中,我们用牦牛模型研究了不同毛囊周期皮肤组织的结构。此外,我们成功建立了牦牛皮肤毛囊相关细胞的体外模型,包括毛乳头细胞(DPC),前脂肪细胞,和成纤维细胞。我们通过简化程序并减少所需的时间来优化DPC培养的显微切割技术。此外,我们改进了将牦牛前脂肪细胞分化为成熟脂肪细胞的方法,从而提高差异化效率。牦牛作为自然模型的引入为探索毛发生长机制提供了宝贵的研究资源,有助于更深入地了解毛囊生物学和再生医学策略的发展。
    To adapt to the extreme conditions of plateau environments, yaks have evolved thick hair, making them an ideal model for investigating the mechanisms involved in hair growth. We can gain valuable insights into how hair follicles develop and their cyclic growth in challenging environments by studying yaks. However, the lack of essential data on yak hair follicle histology and the absence of in vitro cell models for hair follicles serve as a limitation to such research objectives. In this study, we investigated the structure of skin tissue during different hair follicle cycles using the yak model. Additionally, we successfully established in vitro models of hair follicle-associated cells derived from yak skin, including dermal papilla cells (DPCs), preadipocytes, and fibroblasts. We optimized the microdissection technique for DPCs culture by simplifying the procedure and reducing the time required. Furthermore, we improved the methodology used to differentiate yak preadipocytes into mature adipocytes, thus increasing the differentiation efficiency. The introduction of yak as a natural model provides valuable research resources for exploring the mechanisms of hair growth and contributes to a deeper understanding of hair follicle biology and the development of regenerative medicine strategies.
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  • 文章类型: Journal Article
    DNA损伤是干细胞衰老的细胞内在原因之一,这导致分化诱导的皮肤和血液中受损干细胞的去除。饮食限制(DR)延缓了各种物种的衰老,包括几株实验室老鼠。是否,DR具有改善DNA损伤驱动的干细胞衰竭的潜力仍未完全了解。在这项研究中,我们显示DR强烈延长了响应于γ辐射(电离辐射[IR])诱导的C57BL/6J小鼠DNA损伤的头发变白的时间。研究表明DR延长了毛囊的静息期。DR介导的毛囊干细胞(HFSC)静止期延长会阻止头发生长,并阻止HFSC和ckit+黑素细胞在IR反应中的消耗。然而,HFSC静止期的延长也与DNA修复的抑制相关,并且从长远来看不能防止黑色素细胞损失和头发变白。即使在延长的时间后,头发循环也会重新开始。总之,这些结果支持了一个模型,该模型表明营养剥夺可以通过使HFSCs在长时间的静止状态下停止并抑制DNA修复来延迟但不能治愈DNA损伤驱动的黑素细胞灭绝.断开这两种对DR的反应可能有延迟干细胞衰老的潜力。
    DNA damage represents one of the cell intrinsic causes of stem cell aging, which leads to differentiation-induced removal of damaged stem cells in skin and blood. Dietary restriction (DR) retards aging across various species, including several strains of laboratory mice. Whether, DR has the potential to ameliorate DNA damage-driven stem cell exhaustion remains incompletely understood. In this study, we show that DR strongly extends the time to hair graying in response to γ-irradiation (ionizing radiation [IR])-induced DNA damage of C57BL/6 J mice. The study shows that DR prolongs resting phase of hair follicles. DR-mediated prolongation of hair follicle stem cell (HFSC) quiescence blocks hair growth and prevents the depletion of HFSCs and ckit+ melanoblasts in response to IR. However, prolongation of HFSC quiescence also correlates with a suppression of DNA repair and cannot prevent melanoblast loss and hair graying in the long run, when hair cycling is reinitiated even after extended periods of time. Altogether, these results support a model indicating that nutrient deprivation can delay but not heal DNA damage-driven extinction of melanoblasts by stalling HFSCs in a prolonged state of quiescence coupled with inhibition of DNA repair. Disconnecting these two types of responses to DR could have the potential to delay stem cell aging.
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  • 文章类型: Journal Article
    由于患有脱发症的人数稳步增加,这种情况最近受到越来越多的关注。脱发可由多种病理因素引起,环境或心理因素,最终导致毛囊(HF)结构异常或HF再生障碍,特别是毛囊干细胞(HFSC)行为失调。HFSC行为包括激活,增殖和分化。适当的HFSC行为维持持续的毛发周期(HC)。HFSC行为主要受HFSC代谢的影响,老龄化和微环境。在这次审查中,我们总结了最近关于HFSC代谢,老化和微环境会导致头发生长障碍,以及相关基因和信号通路。本文还综述了近年来基于干细胞的毛发组织工程和再生医学治疗脱发的研究进展。确定失调的HFSC行为如何导致脱发将有助于确定潜在的治疗靶标。
    Due to a steady increase in the number of individuals suffering from alopecia, this condition has recently received increasing attention. Alopecia can be caused by various pathological, environmental or psychological factors, eventually resulting in abnormalities in hair follicle (HF) structures or HF regeneration disorders, especially dysregulated hair follicle stem cell (HFSC) behaviour. HFSC behaviour includes activation, proliferation and differentiation. Appropriate HFSC behaviour sustains a persistent hair cycle (HC). HFSC behaviour is mainly influenced by HFSC metabolism, ageing and the microenvironment. In this review, we summarize recent findings on how HFSC metabolism, ageing and the microenvironment give rise to hair growth disorders, as well as related genes and signalling pathways. Recent research on the application of stem cell-based hair tissue engineering and regenerative medicine to treat alopecia is also summarized. Determining how dysregulated HFSC behaviour underlies alopecia would be helpful in identifying potential therapeutic targets.
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  • 文章类型: Journal Article
    在各种类型的脱发中都发现了氧化损伤。作为一种多酚植物抗毒素,白藜芦醇(RSV)被称为抗氧化剂,抗炎和抗凋亡剂。
    因此,我们的目的是研究RSV对头发生长的影响。
    体内C57BL/6小鼠用于评估RSV对毛发周期的影响,头发长度,蒙皮厚度,毛囊直径,头发周期得分和头发周期阶段的百分比。然后通过人毛囊(HF)离体评估毛干长度和毛发周期。使用RTCA评估了用RSV体外培养的人真皮乳头细胞(hDPC)的增殖活性。通过ROS测定试剂盒检查RSV保护hDPC免受H2O2诱导的氧化损伤的能力。
    在剃须的C57BL/6小鼠上,RSV的局部应用显着促进了毛发生长,并刺激了毛发周期从静止期到生长期的转变。离体实验表明,RSV增加了HFs的毛干长度,并延迟了其进入的过程。体外实验表明,RSV可以增殖hDPCs,并防止hDPCs受到H2O2的氧化损伤。
    RSV可以促进头发生长,可能是治疗脱发的潜在候选者。
    UNASSIGNED: Oxidative damage has been found in various types of hair loss. As a polyphenolic phytoalexin, resveratrol (RSV) is known as an antioxidant, anti-inflammatory and anti-apoptotic agent.
    UNASSIGNED: Thus, we aim to examine the effects of RSV on hair growth.
    UNASSIGNED: In vivo C57BL/6 mice were used to evaluate the effects of RSV on hair cycle, hair length, skin thickness, hair follicle diameter, hair cycle score and the percentage of hair cycle stage. Then hair shaft length and hair cycle were evaluated by human hair follicles (HFs) ex vivo. The proliferative activities of human dermal papilla cells (hDPCs) cultured in vitro with RSV were assessed using RTCA. The ability of RSV to protect hDPCs against H2O2-induced oxidative damage is examined by a ROS assay kit.
    UNASSIGNED: Topical application of RSV significantly promoted hair growth and stimulated the transition of hair cycle from telogen into the anagen phase on shaved C57BL/6 mice. Ex vivo experiments showed that RSV increased the hair shaft length of HFs and delayed the entry into catagen. In vitro experiments indicated that RSV proliferated hDPCs and prevented hDPCs from oxidative damage caused by H2O2.
    UNASSIGNED: RSV can promote hair growth and may be a potential candidate for the treatment of hair loss.
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  • 文章类型: Journal Article
    已发现从木兰属中分离的脂质体和厚朴酚具有抗血管生成作用,抗炎和抗肿瘤特性。然而,没有关于它在头发生长中的作用的报告。毛囊是终生循环的细胞器,从生长期经过,capagen和telogen阶段,并由不同的信号通路调节,包括Wnt/β-catenin,缺口,表皮生长因子(EGF)和SonicHegehog(SHH)。Wnt信号对于毛囊胎盘发育的启动和脱发治疗的新潜在靶标至关重要。本研究旨在研究脂质体和厚朴酚(Lip-和厚朴酚)对诱导毛发生长的影响。我们鉴定了通过每日腹部注射脂质体和厚朴酚(Lip-和厚朴酚)处理后,在C57BL/6N小鼠的剃须区域中的毛发提前长大。我们首先证明了Lip-和厚朴酚通过免疫组织化学和免疫荧光染色激活了Wnt3a/β-catenin途径并下调了转化生长因子-β1(TGF-β1)以促进小鼠的毛发生长。这些发现表明,和厚朴酚激活了Wnt/β-catenin途径,并加速了从静止期到生长期的转移,最终促进了头发的生长。
    Liposomal honokiol isolated from the genus Magnolia has been found to have antiangiogenic, anti-inflammatory and antitumor properties. However, there has no report on its role in hair growth. Hair follicles are life-long cycled organelles that go through from anagen, catagen and telogen stages and are regulated by diverse signaling pathways, including Wnt/β-catenin, Notch, Epidermal growth factor (EGF) and Sonic hegehog (SHH). Wnt signals are essential for the initiation of hair follicle placode development and a new potential target of hair loss treatment. This study was designed to investigate the effect of liposomal honokiol (Lip-honokiol) on inducing hair anagen. We identified the hair grew out in advance in the shaving area of C57BL/6N mice after the treatment of liposomal honokiol (Lip-honokiol) by daily abdominal injection. We first demonstrated that Lip-Honokiol activated the Wnt3a/β-catenin pathway and downregulated the transforming growth factor-β1 (TGF-β1) to promote hair growth in mice via immunohistochemistry and immunofluorescence staining. These findings suggest that Lip-honokiol activated the Wnt/β-catenin pathway and accelerated the transfer from the telogen to anagen stage and finally promoted the hair growth.
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  • 文章类型: Journal Article
    BACKGROUND: REGγ acts as a proteasome activating factor mediating proteasome degradation of substrate proteins in an ATP and ubiquitination independent manner and also as an important regulator of cell cycle, proliferation and apoptosis. Hair cycle involves dynamic, continuous morphological changes of three stages (anagen, catagen and telogen).
    OBJECTIVE: The function of REGγ in hair cycling is still unclear.
    METHODS: Here, we used REGγ knockout 293 T cells, inducible 293WT and 293N151Y cell, REGγ knockout mice to identify the novel molecular mechanism of REGγ in regulating hair follicle stem cells.
    RESULTS: In the present study, we found that REGγ deletion markedly delayed the transition of hair follicles from telogen to anagen and hair regeneration in mice. We also observed significant decrease of hair follicle stem cell number, stem-like property and proliferation ability. Interestingly, the results from real-time PCR, FACS, Western Blot and immunofluorescent analysis showed that REGγ deletion could greatly downregulate Lgr5 expression in the hair follicles. Meanwhile, REGγ was demonstrated to directly interact with LHX2 and promotes its degradation. Importantly, REGγ specific deletion in Lgr5+ stem cells induced the marked delay of hair regeneration after depilation.
    CONCLUSIONS: These data together indicate that REGγ was a new mediator of Lgr5 expression in hair follicle at least partly by promoting the degradation of its suppressive transcription factor LHX2. It seemed that REGγ regulated hair anagen entry and hair regrowth by activating Lgr5 positive hair follicle stem cells.
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  • 文章类型: Journal Article
    OBJECTIVE: Hair cycle is regulated by many biological factors. Cathepsins are involved in various physiological processes in human skin. Here, we investigated the cathepsin expression and distribution changes in follicular growth cycles for better understanding the hair cycles and to explore new intervention measures.
    METHODS: The 24 mice (C57BL/6, female, 7-week old) were selected and removed the back hair via rosin/paraffin method. At Day 8, Day 20, and Day 25, biopsy on post-plucking area was done. Immunohistochemical staining, Western blot, and Q-PCR were used to test the cathepsin B/D/L/E.
    RESULTS: In anagen, cathepsins (B, D, L, and E) were distributed in the hair follicle matrix, inner hair root sheath, and hair. In catagen, cathepsins were mainly observed in un-apoptosis inner root sheath and outer root sheath. Expression of cathepsins B-mRNA and L-mRNA was decreased from anagen and catagen to telogen. Cathepsin D-mRNA was increased in catagen and then decreased in telogen. Cathepsin E-mRNA was decreased in catagen and slightly increased in telogen.
    CONCLUSIONS: The distribution and expression of cathepsins B, D, L, and E in hair follicle changed with hair growth process which indicated that cathepsins might act as selectable biomarkers of hair cycle in different stages.
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