PDF(Pubmed)
Abstract：
UNASSIGNED: Thyroxine is important to maintain the normal operation of the body. Both clinical and experimental results show thyroxine is closely related to hair growth, the mechanism of which is not fully understood.
UNASSIGNED: Investigate the effect of thyroxine receptor agonist, TDM10842, for dorsal hair growth in C3H mice and explore its underlying mechanism.
UNASSIGNED: Depilated mice were applied with the TDM10842, vehicle of this drug and without any materials on dorsal skin. RNA-sequencing (RNA-seq) was employed to identify the change in gene expression of skin tissues. Quantitative real-time PCR (rt-PCR) and immunoblotting were conducted to validate key differentially expressed genes (DEGs) between different groups.
UNASSIGNED: The TDM group showed early induction of anagen. 857, 782, and 276 differentially expressed genes were identified between 3 groups. As a critical DEG in group TDM, Pclaf was positively related to the motivation of Wnt/beta-catenin and Hedgehog signaling pathways, with a high expression of Ki67 and cyclinD1.
UNASSIGNED: TDM10842 accelerates the anagen entrance and the potential mechanism might be the activation of Wnt/beta-catenin and Hedgehog pathways. Pclaf serves as a critical molecule involved in pathway activation, and cyclinD1 is an important effector protein downstream of the pathways.
UNASSIGNED: Investigate the effect of thyroxine receptor agonist, TDM10842, for dorsal hair growth in C3H mice and explore its underlying mechanism.
UNASSIGNED: Depilated mice were applied with the TDM10842, vehicle of this drug and without any materials on dorsal skin. RNA-sequencing (RNA-seq) was employed to identify the change in gene expression of skin tissues. Quantitative real-time PCR (rt-PCR) and immunoblotting were conducted to validate key differentially expressed genes (DEGs) between different groups.
UNASSIGNED: The TDM group showed early induction of anagen. 857, 782, and 276 differentially expressed genes were identified between 3 groups. As a critical DEG in group TDM, Pclaf was positively related to the motivation of Wnt/beta-catenin and Hedgehog signaling pathways, with a high expression of Ki67 and cyclinD1.
UNASSIGNED: TDM10842 accelerates the anagen entrance and the potential mechanism might be the activation of Wnt/beta-catenin and Hedgehog pathways. Pclaf serves as a critical molecule involved in pathway activation, and cyclinD1 is an important effector protein downstream of the pathways.
摘要：
■甲状腺素对于维持身体的正常运转很重要。临床和实验结果均显示甲状腺素与毛发生长密切相关,其机制尚未完全理解。
■研究甲状腺素受体激动剂的作用，TDM10842，用于C3H小鼠背毛生长并探讨其潜在机制。
■将脱毛小鼠用TDM10842、该药物的媒介物施用,并且在背部皮肤上没有任何材料。采用RNA测序(RNA-seq)来鉴定皮肤组织的基因表达的变化。进行定量实时PCR(rt-PCR)和免疫印迹以验证不同组之间的关键差异表达基因(DEGs)。
■TDM组显示早期诱导生长期。在3组之间鉴定了857、782和276个差异表达的基因。作为组TDM中的关键DEG，Pclaf与Wnt/β-catenin和Hedgehog信号通路的动机呈正相关,具有高表达的Ki67和cyclinD1。
■TDM10842加速了生长期的进入，潜在的机制可能是Wnt/β-catenin和Hedgehog途径的激活。Pclaf是参与通路激活的关键分子，cyclinD1是该途径下游的重要效应蛋白。
■研究甲状腺素受体激动剂的作用，TDM10842，用于C3H小鼠背毛生长并探讨其潜在机制。
■将脱毛小鼠用TDM10842、该药物的媒介物施用,并且在背部皮肤上没有任何材料。采用RNA测序(RNA-seq)来鉴定皮肤组织的基因表达的变化。进行定量实时PCR(rt-PCR)和免疫印迹以验证不同组之间的关键差异表达基因(DEGs)。
■TDM组显示早期诱导生长期。在3组之间鉴定了857、782和276个差异表达的基因。作为组TDM中的关键DEG，Pclaf与Wnt/β-catenin和Hedgehog信号通路的动机呈正相关,具有高表达的Ki67和cyclinD1。
■TDM10842加速了生长期的进入，潜在的机制可能是Wnt/β-catenin和Hedgehog途径的激活。Pclaf是参与通路激活的关键分子，cyclinD1是该途径下游的重要效应蛋白。
