PDF(Pubmed)
Abstract：
To adapt to the extreme conditions of plateau environments, yaks have evolved thick hair, making them an ideal model for investigating the mechanisms involved in hair growth. We can gain valuable insights into how hair follicles develop and their cyclic growth in challenging environments by studying yaks. However, the lack of essential data on yak hair follicle histology and the absence of in vitro cell models for hair follicles serve as a limitation to such research objectives. In this study, we investigated the structure of skin tissue during different hair follicle cycles using the yak model. Additionally, we successfully established in vitro models of hair follicle-associated cells derived from yak skin, including dermal papilla cells (DPCs), preadipocytes, and fibroblasts. We optimized the microdissection technique for DPCs culture by simplifying the procedure and reducing the time required. Furthermore, we improved the methodology used to differentiate yak preadipocytes into mature adipocytes, thus increasing the differentiation efficiency. The introduction of yak as a natural model provides valuable research resources for exploring the mechanisms of hair growth and contributes to a deeper understanding of hair follicle biology and the development of regenerative medicine strategies.
摘要：
适应高原环境的极端条件,牦牛进化出浓密的头发,使它们成为研究头发生长机制的理想模型。通过研究牦牛，我们可以获得有关毛囊在充满挑战的环境中如何发育及其周期性生长的宝贵见解。然而,缺乏关于牦牛毛囊组织学的基本数据，以及缺乏毛囊的体外细胞模型，这限制了这些研究目标。在这项研究中,我们用牦牛模型研究了不同毛囊周期皮肤组织的结构。此外,我们成功建立了牦牛皮肤毛囊相关细胞的体外模型,包括毛乳头细胞（DPC），前脂肪细胞,和成纤维细胞。我们通过简化程序并减少所需的时间来优化DPC培养的显微切割技术。此外，我们改进了将牦牛前脂肪细胞分化为成熟脂肪细胞的方法，从而提高差异化效率。牦牛作为自然模型的引入为探索毛发生长机制提供了宝贵的研究资源，有助于更深入地了解毛囊生物学和再生医学策略的发展。
