PDF(Pubmed)
Abstract：
UNASSIGNED: Existing research links oxidative stress and inflammation to hair loss. Salvianolic acid B (SAB) is known for its anti-oxidative, anti-inflammatory, and other beneficial pharmacological properties.
UNASSIGNED: To assess the efficacy of SAB in modulating hair growth.
UNASSIGNED: In vivo experiments were conducted using C57BL/6 mice to evaluate the effects of SAB on hair and skin parameters. The study involved ex vivo analysis of human hair follicles (HFs) for hair shaft length and hair growth cycle assessment. In vitro, human dermal papilla cells (hDPCs) were cultured with SAB, and their proliferation, protection against H2O2-induced oxidative damage, and gene/protein expression alterations were examined using various analytical techniques, including Real-Time Cell Analysis (RTCA), DCFH-DA Assay, RNA-seq, and KEGG pathway analysis.
UNASSIGNED: SAB treatment in mice significantly improved hair growth and vascularization by day 21. In human HFs, SAB extended hair shaft length and delayed the transition to the catagen phase. SAB-treated hDPCs showed a notable decrease in the expression of oxidation-antioxidation-related genes and proteins, including reduced phosphorylation levels of ERK and p38.
UNASSIGNED: The study indicates that SAB promotes hDPC proliferation and offers protection against oxidative stress, highlighting its potential as a therapeutic agent for enhancing hair growth and treating hair loss.
UNASSIGNED: To assess the efficacy of SAB in modulating hair growth.
UNASSIGNED: In vivo experiments were conducted using C57BL/6 mice to evaluate the effects of SAB on hair and skin parameters. The study involved ex vivo analysis of human hair follicles (HFs) for hair shaft length and hair growth cycle assessment. In vitro, human dermal papilla cells (hDPCs) were cultured with SAB, and their proliferation, protection against H2O2-induced oxidative damage, and gene/protein expression alterations were examined using various analytical techniques, including Real-Time Cell Analysis (RTCA), DCFH-DA Assay, RNA-seq, and KEGG pathway analysis.
UNASSIGNED: SAB treatment in mice significantly improved hair growth and vascularization by day 21. In human HFs, SAB extended hair shaft length and delayed the transition to the catagen phase. SAB-treated hDPCs showed a notable decrease in the expression of oxidation-antioxidation-related genes and proteins, including reduced phosphorylation levels of ERK and p38.
UNASSIGNED: The study indicates that SAB promotes hDPC proliferation and offers protection against oxidative stress, highlighting its potential as a therapeutic agent for enhancing hair growth and treating hair loss.
摘要：
■现有研究将氧化应激和炎症与脱发联系起来。丹酚酸B（SAB）以其抗氧化作用而闻名，抗炎,和其他有益的药理特性。
■评估SAB在调节毛发生长中的功效。
■使用C57BL/6小鼠进行体内实验，以评估SAB对头发和皮肤参数的影响。该研究涉及人毛囊(HF)的离体分析,用于毛干长度和毛发生长周期评估。体外，人毛乳头细胞（hDPC）用SAB培养，以及它们的扩散，防止H2O2诱导的氧化损伤，使用各种分析技术检查基因/蛋白质表达改变，包括实时细胞分析（RTCA），DCFH-DA测定,RNA-seq,和KEGG通路分析。
■小鼠中的SAB治疗到第21天显着改善了毛发生长和血管形成。在人类HFs中，SAB延长了毛干长度，并延迟了向前阶段的过渡。SAB处理的hDPC显示氧化-抗氧化相关基因和蛋白质的表达显着降低，包括ERK和p38的磷酸化水平降低。
■研究表明，SAB促进hDPC增殖并提供抗氧化应激保护，突出了其作为增强头发生长和治疗脱发的治疗剂的潜力。
■评估SAB在调节毛发生长中的功效。
■使用C57BL/6小鼠进行体内实验，以评估SAB对头发和皮肤参数的影响。该研究涉及人毛囊(HF)的离体分析,用于毛干长度和毛发生长周期评估。体外，人毛乳头细胞（hDPC）用SAB培养，以及它们的扩散，防止H2O2诱导的氧化损伤，使用各种分析技术检查基因/蛋白质表达改变，包括实时细胞分析（RTCA），DCFH-DA测定,RNA-seq,和KEGG通路分析。
■小鼠中的SAB治疗到第21天显着改善了毛发生长和血管形成。在人类HFs中，SAB延长了毛干长度，并延迟了向前阶段的过渡。SAB处理的hDPC显示氧化-抗氧化相关基因和蛋白质的表达显着降低，包括ERK和p38的磷酸化水平降低。
■研究表明，SAB促进hDPC增殖并提供抗氧化应激保护，突出了其作为增强头发生长和治疗脱发的治疗剂的潜力。
