目标:西洋参浆果,在地上种植并在8月收获,是一种潜在的有价值的材料。该研究的目的是分析西洋参浆果中的特定多糖,并通过体内外实验和分子对接来证明其抗炎作用。
方法:脱蛋白和透析后,提取的粗多糖经分离纯化。通过傅里叶变换红外光谱(FT-IR)研究了特定分离多糖的结构,GC-MS和核磁共振(NMR),使用体外和体内模型(Raw264.7细胞和斑马鱼)评估抗炎活性。分子对接用于分析与环氧合酶2(COX-2)的结合能力和相互作用。
结果:从西洋参浆果中分离出一种新型的中性多糖部分(AGBP-A)。结构分析表明,AGBP-A的重均分子量(Mw)为122,988Da,分散指数(Mw/Mn)值为1.59,由阿拉伯糖和半乳糖组成,其核心结构包含→6)-Gal-(1→残基作为骨架,在C3位具有分支取代。由α-L-Ara-(1→,α-L-Ara-(1→,→5)-α-L-Ara-(1→,β-D-Gal-(1→.结果表明,它显着降低了细胞模型中的促炎细胞因子。在斑马鱼模型中,AGBP-A减少了中性粒细胞大量募集到尾侧线神经肥大,暗示炎症的缓解。分子对接用于分析联合能力和与COX-2的相互作用。
结论:我们的研究表明AGBP-A作为一种安全有效的天然抗炎成分具有潜在的疗效。
OBJECTIVE: American ginseng berries, grown in the aerial parts and harvested in August, are a potentially valuable material. The aim of the study was to analyze the specific polysaccharides in American ginseng berries, and to demonstrate the anti-inflammation effect through in vitro and in vivo experiments and molecular docking.
METHODS: After deproteinization and dialysis, the extracted crude polysaccharide was separated and purified. The structure of the specific isolated polysaccharide was investigated by Fourier Transform infrared spectroscopy (FT-IR), GC-MS and nuclear magnetic resonance (NMR), and anti-inflammatory activity was evaluated using in vitro and in vivo models (Raw 264.7 cells and zebrafish). Molecular docking was used to analyze the binding capacity and interaction with cyclooxygenase-2 (COX-2).
RESULTS: A novel neutral polysaccharide fraction (AGBP-A) was isolated from American ginseng berries. The structural analysis demonstrated that AGBP-A had a weight-average molecular weight (Mw) of 122,988 Da with a dispersity index (Mw/Mn) value of 1.59 and was composed of arabinose and galactose with a core structure containing →6)-Gal-(1→ residues as the backbone and a branching substitution at the C3 position. The side-chains comprised of α-L-Ara-(1→, α-L-Ara-(1→, →5)-α-L-Ara-(1→, β-D-Gal-(1→. The results showed that it significantly decreased pro-inflammatory cytokines in the cell model. In a zebrafish model, AGBP-A reduced the massive recruitment of neutrophils to the caudal lateral line neuromast, suggesting the relief of inflammation. Molecular docking was used to analyze the combined capacity and interaction with COX-2.
CONCLUSIONS: Our study indicated the potential efficacy of AGBP-A as a safe and valid natural anti-inflammatory component.