Rotator cuff repair

肩袖修复
  • 文章类型: Journal Article
    背景:在肩袖损伤的临床和动物研究中,功能评估对于评估治疗结果至关重要。虽然步态分析通常用于评估肩袖撕裂的动物模型,它与人类患者的相关性较小,因为人类肩部通常是在非负重状态下评估的。本研究介绍了熟练的触角测试作为大鼠肩部功能评估工具,这允许评估没有承重。
    方法:在对照组中,8只雄性Sprague-Dawley大鼠接受了无修复的肩袖撕裂手术。在肩袖修复组中,20只大鼠在肩袖撕裂后4周接受肩袖修复。对于熟练的触达测试,训练大鼠伸展前肢以获取食物颗粒,以及试验的数量,记录了尝试次数和成功率。在基线时进行步态分析和熟练到达测试,撕裂后4周,修复后1、2、4和8周。重复测量方差分析用于评估时间对肩关节功能的影响。显著性水平设定为0.05。
    结果:熟练的测试需要216小时才能进行,而步态分析需要44小时。在肩袖修复组中,步态表现在修复后1周显著恶化,在修复后4周恢复至撕裂后4周水平。关于熟练的触达测试,尝试的次数,修复后1周的试验数量和成功率下降.随后,修复后2周观察到性能短暂反弹,随后,尝试和试验的数量持续下降。修复后8周,只有成功率恢复到与撕裂后4周相似的水平.
    结论:熟练的伸手测试可以检测肩袖撕裂和修复后的功能缺陷,虽然它需要很高的时间和劳动力成本。
    BACKGROUND: Functional assessments are crucial to evaluate treatment outcomes in clinical and animal studies on rotator cuff injuries. While gait analysis is commonly used to assess animal models of rotator cuff tears, it is less relevant for human patients as the human shoulder is typically assessed in a non-weight-bearing condition. The present study introduces the skilled reaching test as a shoulder functional assessment tool for rats, which allows for evaluation without weight bearing.
    METHODS: In the control group, 8 male Sprague-Dawley rats received rotator cuff tear surgery without repair. In the rotator cuff repair group, 20 rats received rotator cuff repair at 4 weeks post rotator cuff tear. For the skilled reaching test, rats were trained to extend their forelimbs to fetch food pellets, and the number of trials, number of attempts and the success rate were recorded. The gait analysis and skilled reaching test were performed at baseline, 4 weeks post-tear, 1, 2, 4, and 8 weeks post-repair. The repeated measures analysis of variance was used to evaluate the effects of time on the shoulder function. The significance level was set at 0.05.
    RESULTS: The skilled reaching test required 216 h to conduct, while the gait analysis took 44 h. In the rotator cuff repair group, gait performance significantly deteriorated at 1 week post-repair and restored to 4 weeks post-tear levels at 4 weeks post-repair. Regarding the skilled reaching test, the number of attempts, number of trials and the success rate decreased at 1 week post-repair. Subsequently, there was a brief rebound in performance observed at 2 weeks post-repair, followed by a continued decline in the number of attempts and trials. By 8 weeks post-repair, only the success rate had restored to levels similar to those observed at 4 weeks post-tear.
    CONCLUSIONS: The skilled reaching test can detect functional deficiencies following rotator cuff tear and repair, while it requires high time and labour costs.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    背景:手术修复时机对创伤性和非创伤性肩袖损伤(RCI)结局的影响仍然难以捉摸。因此,本研究旨在比较不同时间点创伤性和非创伤性RCI修复后结局的差异.
    方法:研究人群包括87例创伤性和非创伤性RCI患者,他们接受了关节镜肩袖修复,并随访至少6个月。接下来,根据损伤时间(早期修复:3个月内发生;延迟修复:3个月后发生),将创伤组和非创伤组分为亚组.比较手术干预前后的测量结果,以评估RCI持续时间对创伤和非创伤组患者功能状态的影响。主要评价指标包括视觉模拟量表(VAS)疼痛评分,美国肩肘外科医师(ASES)评分,肩关节功能评分恒定,和加州大学,洛杉矶(UCLA)肩膀得分。次要评价指标包括肩部活动范围(ROM),术后肩袖再撕裂率,和接头刚度的发生率。
    结果:在创伤组的40例患者中,22人接受了早期修复,而其余18人接受了延迟修复。在由47名患者组成的非创伤组中,18人接受了早期修复,而其余29人接受了延迟修复。最短临床随访时间为6个月,平均随访10.2个月。术后随访期间,创伤组中有1例和6例接受早期和延迟修复的患者经历了再撕裂,分别。相反,3和8例接受早期和延迟修复的患者在非创伤组中出现了再撕裂,分别。
    结论:创伤性RCI的早期修复产生了更好的结果,包括改进的运动范围,较低的疼痛症状,与延迟修复相比,术后再撕裂的风险较低。此外,非手术治疗被推荐为非创伤性RCI患者的首选治疗方法.
    BACKGROUND: The effects of the timing of surgical repair on the outcomes of traumatic and non-traumatic rotator cuff injuries (RCI) remain elusive. Thus, this study aimed to compare differences in outcomes following the repair of traumatic and non-traumatic RCI at varying time points.
    METHODS: The study population comprised 87 patients with traumatic and non-traumatic RCI who underwent arthroscopic rotator cuff repair and were followed up for a minimum of 6 months. Next, the trauma and the non-trauma groups were stratified into subgroups according to the time of injury (early repair: occurring within 3 months; delayed repair: occurring after 3 months). Measurements before and after surgical interventions were compared to evaluate the effect of the duration of RCI on the functional status of patients in the trauma and non-trauma groups. Primary evaluation indices included the Visual Analog Scale (VAS) pain score, American Shoulder and Elbow Surgeons (ASES) score, Constant shoulder function score, and the University of California, Los Angeles (UCLA) shoulder score. Secondary evaluation indices consisted of shoulder range of motion (ROM), postoperative rotator cuff retear rate, and incidence of joint stiffness.
    RESULTS: Among the 40 patients in the trauma group, 22 underwent early repair, whereas the remaining 18 underwent delayed repair. In the non-trauma group consisting of 47 patients, 18 underwent early repair, whereas the remaining 29 underwent delayed repair. The minimum clinical follow-up time was 6 months, with an average follow-up time of 10.2 months. During postoperative follow-up, 1 and 6 patients who underwent early and delayed repair experienced re-tear in the trauma group, respectively. Contrastingly, 3 and 8 patients who underwent early and delayed repair presented with re-tear in the non-trauma group, respectively.
    CONCLUSIONS: Early repair of traumatic RCI yielded superior outcomes, including improved range of motion, lower pain symptoms, and lower risk of postoperative re-tears compared to delayed repair. Additionally, non-surgical treatment is recommended as the preferred approach for patients with non-traumatic RCI.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    肩袖中的腱-骨界面(TBI)具有较差的内在愈合能力,这增加了肩袖修复(RCR)后再撕裂的风险。然而,促进TBI的再生仍然是一个巨大的临床挑战。在这里,作者建立了一种基于磁播种的新策略来增强TBI再生。
    将超顺磁性氧化铁(SPIO-BMSCs)标记的骨髓间充质干细胞植入双相支架可促进RCR后的腱-骨愈合。
    对照实验室研究。
    用SPIOs标记BMSCs。普鲁士蓝染色,CCK-8试验,蛋白质印迹,通过定量逆转录聚合酶链反应(PCR)来确定SPIOs对细胞生物活性和能力的最佳影响浓度。然后在磁场下将SPIO-BMSC磁性接种到双相支架中。通过扫描电子显微镜评估播种效果,并通过Westernblot和PCR评估了将SPIO-BMSCs接种到支架中后软骨分化的潜在机制。此外,使用组织学分析检查SPIO-BMSC/双相支架对RCR大鼠模型后腱-骨愈合的影响,酶联免疫吸附测定,和生物力学评估。
    用100μg/mLSPIO标记的BMSCs对细胞生物活性和软骨分化能力没有影响。将SPIO-BMSCs磁性接种到双相支架中,通过CDR1as/miR-7/FGF2途径增强SPIO-BMSCs的软骨分化,从而在体外形成TBI。此外,在体内应用具有磁性接种的SPIO-BMSCs的双相支架显示出它们的再生潜力,表明在大鼠肩袖撕裂模型中RCR后,它们可以显着加速和促进TBI愈合,具有优越的生物力学特性。
    将SPIO-BMSCs磁接种到双相支架中增强了接种功效以促进细胞分布和凝聚。该构建体在大鼠肩袖撕裂模型中通过CDR1as/miR-7/FGF2途径增强软骨形成过程,并进一步促进RCR后的腱-骨愈合。
    这项研究提供了一种改善RCR后TBI愈合的替代策略。
    UNASSIGNED: The tendon-bone interface (TBI) in the rotator cuff has a poor intrinsic capacity for healing, which increases the risk of retear after rotator cuff repair (RCR). However, facilitating regeneration of the TBI still remains a great clinical challenge. Herein, the authors established a novel strategy based on magnetic seeding to enhance the TBI regeneration.
    UNASSIGNED: Magnetic seeding bone marrow mesenchymal stem cells labeled with superparamagnetic iron oxide (SPIO-BMSCs) into a biphasic scaffold can promote tendon-bone healing after RCR.
    UNASSIGNED: Controlled laboratory study.
    UNASSIGNED: BMSCs were labeled with SPIOs. Prussian blue staining, CCK-8 tests, Western blot, and quantitative reverse transcription polymerase chain reaction (PCR) were used to determine the optimal effect concentration of SPIOs on cell bioactivities and abilities. Then SPIO-BMSCs were magnetically seeded into a biphasic scaffold under a magnetic field. The seeding efficacy was assessed by a scanning electron microscope, and the potential mechanism in chondrogenic differentiation after seeding SPIO-BMSCs into the scaffold was evaluated by Western blot and PCR. Furthermore, the effect of SPIO-BMSC/biphasic scaffold on tendon-bone healing after RCR using a rat model was examined using histological analysis, enzyme-linked immunosorbent assay, and biomechanical evaluation.
    UNASSIGNED: BMSCs labeled with 100 μg/mL SPIO had no effect on cell bioactivities and the ability of chondrogenic differentiation. SPIO-BMSCs were magnetically seeded into a biphasic scaffold, which offered a high seeding efficacy to enhance chondrogenic differentiation of SPIO-BMSCs via the CDR1as/miR-7/FGF2 pathway for TBI formation in vitro. Furthermore, in vivo application of the biphasic scaffold with magnetically seeded SPIO-BMSCs showed their regenerative potential, indicating that they could significantly accelerate and promote TBI healing with superior biomechanical properties after RCR in a rat rotator cuff tear model.
    UNASSIGNED: Magnetically seeding SPIO-BMSCs into a biphasic scaffold enhanced seeding efficacy to promote cell distribution and condensation. This construct enhanced the chondrogenesis process via the CDR1as/miR-7/FGF2 pathway and further promoted tendon-bone healing after RCR in a rat rotator cuff tear model.
    UNASSIGNED: This study provides an alternative strategy for improving TBI healing after RCR.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

    求助全文

  • 文章类型: Journal Article
    使用透骨方法修复肩袖撕裂已有数十年。直接缝合(DS)技术已广泛用于肩袖撕裂;然而,再撕率相对较高。根据材料的最新发展,缝合锚现在经常用于肩袖修复(RCR)。然而,聚醚醚酮(PEEK)仍可能引起并发症,例如囊肿和骨赘的形成。一些研究已经开发了用于RCR的嵌体缝合(IS)技术。
    要比较3种不同的手术技术-即,DS,IS,和PEEK缝合锚(PSA)-影响RCR后的腱-骨愈合。我们假设IS技术将导致更好的腱-骨愈合,并且修复的结构将类似于正常的发生。
    对照实验室研究。
    在36只六个月大的雄性兔子中产生了急性冈下肌腱撕裂,根据用于RCR的技术将其分为3组:DS,IS,PSA。在术后6周和12周对动物实施安乐死,并进行组织学评估和成像。免疫组化和免疫荧光染色显示相关蛋白的表达。通过生物力学测试评估机械性能。
    在12周时,在3组中观察到了再生。然而,DS组I型胶原含量低于PSA和IS组,这与scleraxis的结果相似。在番红O/快速绿色和天狼星红染色后,DS组显示出明显较差的II型胶原蛋白表达和蛋白聚糖沉积。关于runt相关转录因子2和碱性磷酸酶,与其他两组相比,IS组的表达水平上调。
    与DS技术相比,PSA和IS技术有助于肌腱和纤维软骨再生的成熟,而IS技术特别促进了成骨。
    IS和PSA技术可能更有利于RCR后的腱-骨愈合。
    UNASSIGNED: Rotator cuff tears have been repaired using the transosseous method for decades. The direct suture (DS) technique has been widely used for rotator cuff tears; however, the retear rate is relatively high. Suture anchors are now used frequently for rotator cuff repair (RCR) in accordance with recent developments in materials. However, polyether ether ketone (PEEK) may still cause complications such as the formation of cysts and osteophytes. Some studies have developed the inlay suture (IS) technique for RCR.
    UNASSIGNED: To compare how 3 different surgical techniques-namely, the DS, IS, and PEEK suture anchor (PSA)-affect tendon-bone healing after RCR. We hypothesized that the IS technique would lead to better tendon-to-bone healing and that the repaired structure would be similar to the normal enthesis.
    UNASSIGNED: Controlled laboratory study.
    UNASSIGNED: Acute infraspinatus tendon tears were created in 36 six-month-old male rabbits, which were divided into 3 groups based on the technique used for RCR: DS, IS, and PSA. Animals were euthanized at 6 and 12 weeks postoperatively and underwent a histological assessment and imaging. The expression of related proteins was demonstrated by immunohistochemistry and immunofluorescence staining. Mechanical properties were evaluated by biomechanical testing.
    UNASSIGNED: At 12 weeks, regeneration of the enthesis was observed in the 3 groups. However, the DS group showed a lower type I collagen content than the PSA and IS groups, which was similar to the results for scleraxis. The DS group displayed a significantly inferior type II collagen expression and proteoglycan deposition after safranin O/fast green and sirius red staining. With regard to runt-related transcription factor 2 and alkaline phosphatase, the IS group showed upregulated expression levels compared with the other 2 groups.
    UNASSIGNED: Compared with the DS technique, the PSA and IS techniques contributed to the improved maturation of tendons and fibrocartilage regeneration, while the IS technique particularly promoted osteogenesis at the enthesis.
    UNASSIGNED: The IS and PSA techniques may be more beneficial for tendon-bone healing after RCR.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

    求助全文

  • 文章类型: English Abstract
    UNASSIGNED: To analyze the effect of stump-preserving repair on rotator cuff healing and shoulder function for degenerative total rotator cuff tears.
    UNASSIGNED: A clinical data of 152 patients with degenerative total rotator cuff tears, who underwent arthroscopic repair between April 2019 and May 2022, was retrospectively analyzed. There were 76 males and 76 females with an average age of 55.4 years (range, 24-78 years). MRI was performed at 6 months postoperatively to evaluate the rotator cuff healing according to the Sugaya classification. Pre- and intra-operative related factors were included for univariate analysis, including age (≥60 years/<60 years), gender (male/female), passive activity disorder (yes/no), disease duration (≤3 months/>3 months), stump-preserving repair (yes/no), use of suture bridge technique (yes/no), shoulder joint abduction angle at knotting (<45°/≥45°), acromioplasty (yes/no), glucocorticoid injection (yes/no), time for patients to start postoperative passive exercise (≤2 weeks/>2 weeks), and time for patients to start postoperative active exercise (≤3 months/>3 months). The influencing factors of tendon healing were screened; further logistic regression was used to conduct multivariate analysis to screen for risk factors. Two sets of data were balanced by propensity score matching. The American Shoulder and Elbow Surgeons (ASES) score and Constant-Murley score of shoulder joint function at 6 and 12 months postoperatively, as well as rotator cuff healing rate at 6 months postoperatively, were compared between groups based on whether or not stump-preserving repair was used.
    UNASSIGNED: All patients were followed up 12-33 months (mean, 23.8 months). MRI at 25-31 weeks postoperatively showed the 121 cases of rotator cuff healing and 31 cases of non healing. Univariate analysis showed that the disease duration, stump-preserving repair, shoulder joint abduction angle at knotting, and the time for patients to start postoperative active exercise were the influencing factors of rotator cuff healing ( P<0.05). Multivariate analysis showed that non-stump-preserving repair, shoulder abduction angle more than 45° at knotting, and the time to start active exercise within 3 months postoperatively were risk factors affecting rotator cuff healing ( P<0.05). A total of 51 pairs of cases were matched based on the grouping criteria of whether the disease duration exceeded 3 months, whether the shoulder abduction angle at knotting exceeded 45°, and whether the time to start postoperative active exercise exceeded 3 months. The rotator cuff healing rate, ASES score, and Constant-Murley score of the preserving repair group at 6 months postoperatively were superior to those of the non-preserving repair group, and the differences were significant ( P<0.05). There was no significant difference in ASES score and Constant-Murley score between the two groups at 12 months postoperatively ( P>0.05).
    UNASSIGNED: For degenerative total rotator cuff tears, the stump-preserving repair can shorten the healing time and promote the shoulder function recovery, but has no significant effect on shoulder function at 1 year postoperatively.
    UNASSIGNED: 探讨保留足印区残端的肩袖修补术对退行性肩袖全层撕裂术后肩袖愈合以及肩关节功能的影响。.
    UNASSIGNED: 回顾性分析2019年4月—2022年5月152例接受关节镜下肩袖修补术的退行性肩袖全层撕裂患者临床资料。男76例,女76例;年龄24~78岁,平均55.4岁。术后6个月复查MRI,根据Sugaya分型评估肩袖愈合情况。对年龄(≥60岁/<60岁)、性别(男/女)、被动活动受限(是/否)、病程(≤3个月/>3个月)、保残修补(是/否)、缝线桥技术固定(是/否)、打结时肩关节外展角度(<45°/≥45°)、骨性肩峰成形(是/否)、治疗期间注射糖皮质激素(是/否)、术后开始被动锻炼时间(≤2周/>2周)、术后开始主动锻炼时间(≤3个月/>3个月)进行单因素分析,初步筛选肩袖愈合的影响因素;进一步采用logistic回归进行多因素分析,筛选危险因素。以是否使用保残修补作为分组条件,使用倾向性评分匹配平衡两组数据,对术后6、12个月肩关节功能美国肩肘外科协会评分(ASES)、Constant-Murley评分以及术后6个月肩袖愈合进行组间比较。.
    UNASSIGNED: 术后152例患者均获随访,随访时间12~33个月,平均23.8个月。术后25~31周复查MRI示肩袖愈合121例、未愈合31例。单因素分析示病程、保残修补、打结时肩关节外展角度及术后开始主动锻炼时间是修补术后肩袖愈合的影响因素( P<0.05)。多因素分析示术中不保残修补、打结时肩关节外展角度≥45° 及术后3个月内开始主动锻炼是影响肩袖愈合的危险因素( P<0.05)。以是否保残修补为分组条件,以病程是否超过3个月、打结时肩外展角度是否超过45° 及术后开始主动锻炼时间是否超过3个月为筛选条件,共匹配51对患者。保残组术后6个月肩袖愈合率、ASES评分及Constant-Murley评分均优于非保残组,差异有统计学意义( P<0.05);两组术后12个月ASES评分及Constant-Murley评分差异无统计学意义( P>0.05)。.
    UNASSIGNED: 对于退行性全层肩袖撕裂患者,与不保留残端修补相比,保留足印区残端的肩袖修补术可以缩短肩袖愈合时间,肩关节功能恢复更快,但术后1年肩关节功能无明显差异。.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    探讨关节镜下三联松解术联合肩袖修补术治疗肩袖损伤合并冻结肩的效果及对肩关节活动范围和疼痛评分的影响。和血清疼痛介质的水平。
    这是前瞻性研究。前瞻性选取2020年12月至2022年12月甘肃中医药大学第三附属医院收治的肩袖损伤合并肩周炎患者132例,按照随机数字表法分为对照组(n=67)和观察组(n=65)。对照组患者采用单纯关节镜下肩袖修补术,观察组采用关节镜下三联松解术联合肩袖修补术,比较两组的手术效果。
    治疗后三个月,外部旋转,内部旋转,绑架,前屈,β-内啡肽(β-EP),观察组前列腺素E2(PGE2)和P物质(SP)均优于对照组(P<0.05),而受影响的肢体在内部旋转中的负重强度,外旋和前屈均高于对照组(P<0.05)。同时,治疗后1个月和3个月,观察组视觉模拟评分(VAS)评分低于对照组,而加州大学洛杉矶分校肩关节评定量表(UCLA)评分和Constant-Murley评分(CMS)均高于对照组(P<0.05)。
    关节镜下三联松解术结合肩袖修复可改善肩袖损伤合并肩周炎患者的各种效果,例如改善患肢的肌肉力量和改善疼痛介质的水平。
    UNASSIGNED: To investigate the effect of arthroscopic triple release combined with rotator cuff repair in the treatment of rotator cuff injury combined with frozen shoulder and its influence on the range of motion and pain score of shoulder joint, and the levels of serum pain mediators.
    UNASSIGNED: This was prospective study. A total of 132 patients with rotator cuff injury combined with frozen shoulder admitted to The Third Affiliated Hospital of Gansu University of Traditional Chinese Medicine from December 2020 to December 2022 were prospectively selected and divided into two groups according to the random number table method: control group (n=67) and observation group (n=65). Patients in the control group were treated with arthroscopic rotator cuff repair alone, while those in the observation group were treated with arthroscopic triple release combined with rotator cuff repair, and the surgical effects of the two groups were compared.
    UNASSIGNED: Three months after treatment, the external rotation, internal rotation, abduction, forward flexion, β-endorphin(β-EP), prostagranin E2 (PGE2) and substance P(SP)in the observation group were better than those in the control group (P<0.05), while the weight-bearing strength of the affected limb in internal rotation, external rotation and forward flexion was higher than that of the control group(P<0.05). Meanwhile, the Visual Analogue Scale (VAS) score of the observation group was lower than that of the control group at one month and three months after treatment, while the University of California at Los Angeles shoulder rating scale (UCLA) score and Constant-Murley Score (CMS) were higher than those of the control group (P< 0.05).
    UNASSIGNED: Arthroscopic triple release combined with rotator cuff repair improves various effects for patients with rotator cuff injury combined with frozen shoulder, such as ameliorating the muscle strength of the affected limb and improving the level of pain mediators.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    肩痛和残疾是老年人群普遍存在的问题,肩袖撕裂(RCT)是主要原因之一。虽然手术治疗取得了一定的成功,高术后再撕裂率仍然是一个巨大的挑战,尤其是老年患者。衰老相关的肌肉退化,肌腱,腱-骨发生,骨在RCT的发展和预后中起着至关重要的作用。研究表明,衰老会加剧肌肉萎缩和脂肪浸润,改变肌腱结构和生物力学特性,加剧了变性,降低骨密度.尽管最近的研究有助于理解衰老相关RCT的病理生理机制,对这一主题仍然缺乏全面系统的审查。因此,本文就其病理生理变化及其临床意义作一综述,以及与衰老相关的RCT的分子机制,目的是探讨新的治疗方法,以减少与衰老相关的RCT的发生并改善老年患者的术后预后。
    Shoulder pain and disabilities are prevalent issues among the elderly population, with rotator cuff tear (RCT) being one of the leading causes. Although surgical treatment has shown some success, high postoperative retear rates remain a great challenge, particularly in elderly patients. Aging-related degeneration of muscle, tendon, tendon-to-bone enthesis, and bone plays a critical role in the development and prognosis of RCT. Studies have demonstrated that aging worsens muscle atrophy and fatty infiltration, alters tendon structure and biomechanical properties, exacerbates enthesis degeneration, and reduces bone density. Although recent researches have contributed to understanding the pathophysiological mechanisms of aging-related RCT, a comprehensive systematic review of this topic is still lacking. Therefore, this article aims to present a review of the pathophysiological changes and their clinical significance, as well as the molecular mechanisms underlying aging-related RCT, with the goal of shedding light on new therapeutic approaches to reduce the occurrence of aging-related RCT and improve postoperative prognosis in elderly patients.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

    求助全文

  • 文章类型: English Abstract
    UNASSIGNED: To explore the effect of chitosan (CS) hydrogel loaded with tendon-derived stem cells (TDSCs; hereinafter referred to as TDSCs/CS hydrogel) on tendon-to-bone healing after rotator cuff repair in rabbits.
    UNASSIGNED: TDSCs were isolated from the rotator cuff tissue of 3 adult New Zealand white rabbits by Henderson step-by-step enzymatic digestion method and identified by multidirectional differentiation and flow cytometry. The 3rd generation TDSCs were encapsulated in CS to construct TDSCs/CS hydrogel. The cell counting kit 8 (CCK-8) assay was used to detect the proliferation of TDSCs in the hydrogel after 1-5 days of culture in vitro, and cell compatibility of TDSCs/CS hydrogel was evaluated by using TDSCs alone as control. Another 36 adult New Zealand white rabbits were randomly divided into 3 groups ( n=12): rotator cuff repair group (control group), rotator cuff repair+CS hydrogel injection group (CS group), and rotator cuff repair+TDSCs/CS hydrogel injection group (TDSCs/CS group). After establishing the rotator cuff repair models, the corresponding hydrogel was injected into the tendon-to-bone interface in the CS group and TDSCs/CS group, and no other treatment was performed in the control group. The general condition of the animals was observed after operation. At 4 and 8 weeks, real-time quantitative PCR (qPCR) was used to detect the relative expressions of tendon forming related genes (tenomodulin, scleraxis), chondrogenesis related genes (aggrecan, sex determining region Y-related high mobility group-box gene 9), and osteogenesis related genes (alkaline phosphatase, Runt-related transcription factor 2) at the tendon-to-bone interface. At 8 weeks, HE and Masson staining were used to observe the histological changes, and the biomechanical test was used to evaluate the ultimate load and the failure site of the repaired rotator cuff to evaluate the tendon-to-bone healing and biomechanical properties.
    UNASSIGNED: CCK-8 assay showed that the CS hydrogel could promote the proliferation of TDSCs ( P<0.05). qPCR results showed that the expressions of tendon-to-bone interface related genes were significantly higher in the TDSCs/CS group than in the CS group and control group at 4 and 8 weeks after operation ( P<0.05). Moreover, the expressions of tendon-to-bone interface related genes at 8 weeks after operation were significantly higher than those at 4 weeks after operation in the TDSCs/CS group ( P<0.05). Histological staining showed the clear cartilage tissue and dense and orderly collagen formation at the tendon-to-bone interface in the TDSCs/CS group. The results of semi-quantitative analysis showed that compared with the control group, the number of cells, the proportion of collagen fiber orientation, and the histological score in the TDSCs/CS group increased, the vascularity decreased, showing significant differences ( P<0.05); compared with the CS group, the proportion of collagen fiber orientation and the histological score in the TDSCs/CS group significantly increased ( P<0.05), while there was no significant difference in the number of cells and vascularity ( P>0.05). All samples in biomechanical testing failed at the repair site during the testing process. The ultimate load of the TDSCs/CS group was significantly higher than that of the control group ( P<0.05), but there was no significant difference compared to the CS group ( P>0.05).
    UNASSIGNED: TDSCs/CS hydrogel can induce cartilage regeneration to promote rotator cuff tendon-to-bone healing.
    UNASSIGNED: 探讨以壳聚糖(chitosan,CS)负载肌腱干细胞(tendon-derived stem cells,TDSCs)构建的可注射型水凝胶(以下简称TDSCs/CS水凝胶)促进兔肩袖腱-骨愈合的效果。.
    UNASSIGNED: 取3只成年新西兰大白兔肩袖组织,采用Henderson分步酶消化法分离培养TDSCs,并经多向分化及流式细胞术鉴定。以CS包裹第3代TDSCs构建TDSCs/CS水凝胶,体外培养1~5 d以细胞计数试剂盒8(cell counting kit 8,CCK-8)法检测水凝胶中TDSCs增殖情况,并以单纯TDSCs作为对照,评估TDSCs/CS水凝胶细胞相容性。另取36只成年新西兰大白兔,随机分为3组( n=12),分别为肩袖修复组(对照组)、肩袖修复+CS水凝胶注射组(CS组)、肩袖修复+DSCs/CS水凝胶注射组(TDSCs/CS组)。3组建立肩袖损伤+单排技术修复模型后,CS组及TDSCs/CS组将对应水凝胶注入腱-骨界面处修复肩袖,对照组不作其他处理。术后观察动物一般情况,于4、8周取材,实时定量PCR(real-time quantitative PCR,qPCR)检测腱-骨界面成肌腱相关基因(腱调蛋白、转录因子),成软骨相关基因(蛋白聚糖、性别决定区Y框蛋白9),成骨相关基因(ALP、人Runt相关转录因子2)表达;另于8周取材行HE、Masson染色观察并行组织学半定量评分,生物力学测试修复肩袖极限载荷和失效部位,评价组织愈合情况和生物力学特性改变。.
    UNASSIGNED: CCK-8法检测示共培养后CS水凝胶可促进TDSCs增殖( P<0.05)。术后各组动物均存活至实验完成。术后4、8周,TSDCs/CS组的成肌腱、成软骨、成骨相关基因相对表达量均高于CS组和对照组( P<0.05),TSDCs/CS组组内术后8周上述基因相对表达量亦高于术后4周( P<0.05)。组织学染色示TDSCs/CS组腱-骨界面处有清晰的软骨组织和致密有序胶原形成,半定量分析结果示TDSCs/CS组与对照组相比,细胞数量、胶原纤维分布以及组织学评分增高( P<0.05),血管数量降低( P<0.05);TDSCs/CS组与CS组相比,胶原纤维分布以及组织学评分均增高( P<0.05),而细胞数量、血管数量差异无统计学意义( P>0.05)。生物力学检测示所有样本测试过程中均在修复部位失效,TDSCs/CS组极限载荷高于对照组( P<0.05),但与CS组比较差异无统计学意义( P>0.05)。.
    UNASSIGNED: TDSCs/CS水凝胶可以诱导兔肩袖肌腱和骨之间的软骨再生,促进腱-骨愈合。.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Meta-Analysis
    目的:系统评价肥胖对肩袖修复的影响。
    方法:从PubMed,Embase,科克伦图书馆,WebofScience,中国生物医药(CBM),CNKI,万方和VIP数据库从建库至2022年8月1日,由两位作者根据纳入和排除标准独立筛选。尾注X9和Excel2019用于文献提取,管理和数据输入,采用纽卡斯尔-渥太华量表(NOS)对纳入文献进行质量评价。使用STATA16.0和RevMan5.4软件评估术后再撕裂率,再手术率,并发症发生率,美国肩肘外科医师(ASES)评分,视觉模拟量表(VAS),分析肩关节疼痛的手术时间和外旋转角度。
    结果:共纳入13篇文献,包括6项回顾性研究,5个病例对照研究,1项前瞻性队列研究,和1份全文不可用的研究摘要,85503例患者(肥胖组31973例,非肥胖组53530例)。Meta分析显示两组患者的再撕裂率差异有统计学意义[OR=2.58,95CI(1.23,5.41),P=0.01],再手术率[OR=1.31,95CI(1.21,1.42),P<0.00],并发症发生率[OR=1.57,95CI(1.31,1.87),P=0.00],ASES评分[MD=-3.59,95CI(-5.45,-1.74),P=0.00],和VAS[MD=0.24,95CI(0.00,0.49),P=0.05。两组手术时间无差异[MD=6.03,95CI(-7.63,19.69),P=0.39],肩关节外旋角度[MD=-1.79,95CI(-5.30,1.71),P=0.32]。
    结论:肥胖与更高的再撕裂率相关,复活,并发症,肩袖修复后肩关节功能较差和疼痛。
    OBJECTIVE: To systematically evaluate obesity on the outcome of rotator cuff repair.
    METHODS: Literatures on the relationship between obesity and outcomes after rotator cuff repair were searched from PubMed, Embase, Cochrane Library, Web of Science, China biology medicine(CBM), CNKI, Wanfang and VIP databases from building database to August 1, 2022, and were screened independently by two authors according to inclusion and exclusion criteria. Endnote X9 and Excel 2019 were used for literature extraction, management and data entry, and Newcastle-Ottawa Scale (NOS) was used to evaluate quality of the included literatures. STATA 16.0 and RevMan 5.4 softwares were used to evaluate postoperative retear rate, reoperation rate, complication rate, American Shoulder and Elbow Surgeons (ASES) score, visual analogue scale (VAS), operative time and external rotation angle of shoulder joint pain were analyzed.
    RESULTS: Totally 13 literatures were included, including 6 retrospective studies, 5 case-control studies, 1 prospective cohort study, and 1 abstract of a study for which the full text was not available, with 85 503 patients (31 973 in obese group and 53 530 in non-obese group). Meta-analysis showed there were statistical differences between two groups in retear rate [OR=2.58, 95%CI(1.23, 5.41), P=0.01], reoperation rate[OR=1.31, 95%CI(1.21, 1.42), P<0.00], complication rate [OR=1.57, 95%CI(1.31, 1.87), P=0.00], ASES score[MD=-3.59, 95%CI(-5.45, -1.74), P=0.00], and VAS[MD=0.24, 95%CI(0.00, 0.49), P=0.05]. While there were no differences between two groups in operative time[MD=6.03, 95%CI(-7.63, 19.69), P=0.39], external rotation angle of shoulder joint[MD=-1.79, 95%CI(-5.30, 1.71), P=0.32].
    CONCLUSIONS: Obesity is associated with higher rates of retear, resurgery, complications, poorer shoulder function and pain after rotator cuff repair.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

    求助全文

  • 文章类型: Observational Study
    背景:关节镜手术已被确立为治疗肩袖撕裂的有效干预措施。这项研究的主要目的是分析使用单排铆钉固定和双排铆钉固定技术进行肩袖修复手术后外侧肩峰角(LAA)的修改。此外,我们试图探讨LAA对肩袖修复手术预后的影响.
    方法:这项观察性研究回顾性纳入了105例诊断为退行性肩袖撕裂的患者,这些患者在2016年至2019年间接受了关节镜肩袖修复。排除两名肩胛骨下或上唇前路和后路(SLAP)撕裂患者后,以及三名失去随访的患者,纳入一组100例患者进行临床和影像学评估.在这些人中,50人被分配到双排修复组,而其余50人组成单行修复组。手术后不少于24个月进行双侧肩部磁共振成像(MRI)扫描。经验丰富的关节镜外科医生,对左心耳测量视而不见,使用对照MRI评估旋转器间隔(RI)。使用加利福尼亚大学进行功能评估,洛杉矶(UCLA)快速残疾的肩膀和手臂,肩和手(qDASH)得分。采用依赖样本的Wilcoxon符号秩检验比较干预前后组的数据。计算皮尔逊相关系数以评估不同参数之间的关系。
    结果:研究人群包括73名女性患者和27名男性患者,平均年龄58.32±5.29岁,平均随访时间25.88±8.11个月。术前,平均左心耳为75.81°±11.28°,RI为4.78±0.62,UCLA评分为17.54±2.44,qDASH评分为2.45±0.25。平均泪液大小为8.95±2.11mm。在术前和术后测量之间观察到LAA的统计学差异,双行修复组比单行修复组表现出更大的LAA。最后,LAA,RI,24个月随访后的qDASH评分。
    结论:根据我们的发现,使用双排铆钉固定比单排铆钉固定具有更大的LAA角。此外,LAA的这种保留与肩关节功能恢复显著相关.
    BACKGROUND: Arthroscopic surgery has been established as an efficacious intervention for the treatment of rotator cuff tears. The primary aim of this study was to analyze the modifications in the lateral acromial angle (LAA) subsequent to rotator cuff repair surgery using single-row rivet fixation and double-row rivet fixation techniques. Furthermore, we sought to investigate the influence of LAA on the prognosis of rotator cuff repair surgery.
    METHODS: This observational study retrospectively enrolled 105 patients diagnosed with degenerative rotator cuff tears who underwent arthroscopic rotator cuff repair between 2016 and 2019. Following the exclusion of two patients with subscapularis or superior labrum anterior and posterior (SLAP) tears, as well as three patients who were lost to follow-up, a cohort of 100 patients was included for clinical and imaging evaluation. Among these individuals, 50 were assigned to the double-row repair group, whereas the remaining 50 comprised the single-row repair group. Bilateral shoulder magnetic resonance imaging (MRI) scans were conducted no less than 24 months post-surgery. Experienced arthroscopic surgeons, blinded to the LAA measurements, assessed the rotator interval (RI) using a control MRI. Functional assessment was performed using the University of California, Los Angeles (UCLA) quick disability of the shoulder and arm, shoulder and hand (qDASH) score. The Wilcoxon signed-rank test for dependent samples was employed to compare data between the pre- and post-intervention groups. Pearson correlation coefficients were calculated to evaluate the relationship between different parameters.
    RESULTS: The study population consisted of 73 female patients and 27 male patients, with a mean age of 58.32 ± 5.29 years and a mean follow-up duration of 25.88 ± 8.11 months. Preoperatively, the mean LAA was 75.81° ± 11.28°, RI was 4.78 ± 0.62, UCLA score was 17.54 ± 2.44, and qDASH score was 2.45 ± 0.25. The average tear size was 8.95 ± 2.11 mm. A statistically significant difference in LAA was observed between the preoperative and postoperative measurements, with the double-row repair group exhibiting a greater LAA than the single-row repair group. Finally, a significant correlation was identified between LAA, RI, and qDASH scores after a 24-month follow-up period.
    CONCLUSIONS: According to our findings, the utilization of double-row rivet fixation has a greater LAA angle than single-row rivet fixation. Moreover, this preservation of LAA is significantly associated with the functional recovery of the shoulder joint.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

公众号