Abstract：
UNASSIGNED: The tendon-bone interface (TBI) in the rotator cuff has a poor intrinsic capacity for healing, which increases the risk of retear after rotator cuff repair (RCR). However, facilitating regeneration of the TBI still remains a great clinical challenge. Herein, the authors established a novel strategy based on magnetic seeding to enhance the TBI regeneration.
UNASSIGNED: Magnetic seeding bone marrow mesenchymal stem cells labeled with superparamagnetic iron oxide (SPIO-BMSCs) into a biphasic scaffold can promote tendon-bone healing after RCR.
UNASSIGNED: Controlled laboratory study.
UNASSIGNED: BMSCs were labeled with SPIOs. Prussian blue staining, CCK-8 tests, Western blot, and quantitative reverse transcription polymerase chain reaction (PCR) were used to determine the optimal effect concentration of SPIOs on cell bioactivities and abilities. Then SPIO-BMSCs were magnetically seeded into a biphasic scaffold under a magnetic field. The seeding efficacy was assessed by a scanning electron microscope, and the potential mechanism in chondrogenic differentiation after seeding SPIO-BMSCs into the scaffold was evaluated by Western blot and PCR. Furthermore, the effect of SPIO-BMSC/biphasic scaffold on tendon-bone healing after RCR using a rat model was examined using histological analysis, enzyme-linked immunosorbent assay, and biomechanical evaluation.
UNASSIGNED: BMSCs labeled with 100 μg/mL SPIO had no effect on cell bioactivities and the ability of chondrogenic differentiation. SPIO-BMSCs were magnetically seeded into a biphasic scaffold, which offered a high seeding efficacy to enhance chondrogenic differentiation of SPIO-BMSCs via the CDR1as/miR-7/FGF2 pathway for TBI formation in vitro. Furthermore, in vivo application of the biphasic scaffold with magnetically seeded SPIO-BMSCs showed their regenerative potential, indicating that they could significantly accelerate and promote TBI healing with superior biomechanical properties after RCR in a rat rotator cuff tear model.
UNASSIGNED: Magnetically seeding SPIO-BMSCs into a biphasic scaffold enhanced seeding efficacy to promote cell distribution and condensation. This construct enhanced the chondrogenesis process via the CDR1as/miR-7/FGF2 pathway and further promoted tendon-bone healing after RCR in a rat rotator cuff tear model.
UNASSIGNED: This study provides an alternative strategy for improving TBI healing after RCR.
UNASSIGNED: Magnetic seeding bone marrow mesenchymal stem cells labeled with superparamagnetic iron oxide (SPIO-BMSCs) into a biphasic scaffold can promote tendon-bone healing after RCR.
UNASSIGNED: Controlled laboratory study.
UNASSIGNED: BMSCs were labeled with SPIOs. Prussian blue staining, CCK-8 tests, Western blot, and quantitative reverse transcription polymerase chain reaction (PCR) were used to determine the optimal effect concentration of SPIOs on cell bioactivities and abilities. Then SPIO-BMSCs were magnetically seeded into a biphasic scaffold under a magnetic field. The seeding efficacy was assessed by a scanning electron microscope, and the potential mechanism in chondrogenic differentiation after seeding SPIO-BMSCs into the scaffold was evaluated by Western blot and PCR. Furthermore, the effect of SPIO-BMSC/biphasic scaffold on tendon-bone healing after RCR using a rat model was examined using histological analysis, enzyme-linked immunosorbent assay, and biomechanical evaluation.
UNASSIGNED: BMSCs labeled with 100 μg/mL SPIO had no effect on cell bioactivities and the ability of chondrogenic differentiation. SPIO-BMSCs were magnetically seeded into a biphasic scaffold, which offered a high seeding efficacy to enhance chondrogenic differentiation of SPIO-BMSCs via the CDR1as/miR-7/FGF2 pathway for TBI formation in vitro. Furthermore, in vivo application of the biphasic scaffold with magnetically seeded SPIO-BMSCs showed their regenerative potential, indicating that they could significantly accelerate and promote TBI healing with superior biomechanical properties after RCR in a rat rotator cuff tear model.
UNASSIGNED: Magnetically seeding SPIO-BMSCs into a biphasic scaffold enhanced seeding efficacy to promote cell distribution and condensation. This construct enhanced the chondrogenesis process via the CDR1as/miR-7/FGF2 pathway and further promoted tendon-bone healing after RCR in a rat rotator cuff tear model.
UNASSIGNED: This study provides an alternative strategy for improving TBI healing after RCR.
摘要：
■肩袖中的腱-骨界面（TBI）具有较差的内在愈合能力，这增加了肩袖修复（RCR）后再撕裂的风险。然而,促进TBI的再生仍然是一个巨大的临床挑战。在这里，作者建立了一种基于磁播种的新策略来增强TBI再生。
■将超顺磁性氧化铁（SPIO-BMSCs）标记的骨髓间充质干细胞植入双相支架可促进RCR后的腱-骨愈合。
■对照实验室研究。
■用SPIOs标记BMSCs。普鲁士蓝染色,CCK-8试验,蛋白质印迹,通过定量逆转录聚合酶链反应（PCR）来确定SPIOs对细胞生物活性和能力的最佳影响浓度。然后在磁场下将SPIO-BMSC磁性接种到双相支架中。通过扫描电子显微镜评估播种效果，并通过Westernblot和PCR评估了将SPIO-BMSCs接种到支架中后软骨分化的潜在机制。此外，使用组织学分析检查SPIO-BMSC/双相支架对RCR大鼠模型后腱-骨愈合的影响，酶联免疫吸附测定，和生物力学评估。
■用100μg/mLSPIO标记的BMSCs对细胞生物活性和软骨分化能力没有影响。将SPIO-BMSCs磁性接种到双相支架中，通过CDR1as/miR-7/FGF2途径增强SPIO-BMSCs的软骨分化，从而在体外形成TBI。此外，在体内应用具有磁性接种的SPIO-BMSCs的双相支架显示出它们的再生潜力,表明在大鼠肩袖撕裂模型中RCR后，它们可以显着加速和促进TBI愈合，具有优越的生物力学特性。
■将SPIO-BMSCs磁接种到双相支架中增强了接种功效以促进细胞分布和凝聚。该构建体在大鼠肩袖撕裂模型中通过CDR1as/miR-7/FGF2途径增强软骨形成过程，并进一步促进RCR后的腱-骨愈合。
■这项研究提供了一种改善RCR后TBI愈合的替代策略。
■将超顺磁性氧化铁（SPIO-BMSCs）标记的骨髓间充质干细胞植入双相支架可促进RCR后的腱-骨愈合。
■对照实验室研究。
■用SPIOs标记BMSCs。普鲁士蓝染色,CCK-8试验,蛋白质印迹,通过定量逆转录聚合酶链反应（PCR）来确定SPIOs对细胞生物活性和能力的最佳影响浓度。然后在磁场下将SPIO-BMSC磁性接种到双相支架中。通过扫描电子显微镜评估播种效果，并通过Westernblot和PCR评估了将SPIO-BMSCs接种到支架中后软骨分化的潜在机制。此外，使用组织学分析检查SPIO-BMSC/双相支架对RCR大鼠模型后腱-骨愈合的影响，酶联免疫吸附测定，和生物力学评估。
■用100μg/mLSPIO标记的BMSCs对细胞生物活性和软骨分化能力没有影响。将SPIO-BMSCs磁性接种到双相支架中，通过CDR1as/miR-7/FGF2途径增强SPIO-BMSCs的软骨分化，从而在体外形成TBI。此外，在体内应用具有磁性接种的SPIO-BMSCs的双相支架显示出它们的再生潜力,表明在大鼠肩袖撕裂模型中RCR后，它们可以显着加速和促进TBI愈合，具有优越的生物力学特性。
■将SPIO-BMSCs磁接种到双相支架中增强了接种功效以促进细胞分布和凝聚。该构建体在大鼠肩袖撕裂模型中通过CDR1as/miR-7/FGF2途径增强软骨形成过程，并进一步促进RCR后的腱-骨愈合。
■这项研究提供了一种改善RCR后TBI愈合的替代策略。
