PDF(Pubmed)
Abstract：
BACKGROUND: Functional assessments are crucial to evaluate treatment outcomes in clinical and animal studies on rotator cuff injuries. While gait analysis is commonly used to assess animal models of rotator cuff tears, it is less relevant for human patients as the human shoulder is typically assessed in a non-weight-bearing condition. The present study introduces the skilled reaching test as a shoulder functional assessment tool for rats, which allows for evaluation without weight bearing.
METHODS: In the control group, 8 male Sprague-Dawley rats received rotator cuff tear surgery without repair. In the rotator cuff repair group, 20 rats received rotator cuff repair at 4 weeks post rotator cuff tear. For the skilled reaching test, rats were trained to extend their forelimbs to fetch food pellets, and the number of trials, number of attempts and the success rate were recorded. The gait analysis and skilled reaching test were performed at baseline, 4 weeks post-tear, 1, 2, 4, and 8 weeks post-repair. The repeated measures analysis of variance was used to evaluate the effects of time on the shoulder function. The significance level was set at 0.05.
RESULTS: The skilled reaching test required 216 h to conduct, while the gait analysis took 44 h. In the rotator cuff repair group, gait performance significantly deteriorated at 1 week post-repair and restored to 4 weeks post-tear levels at 4 weeks post-repair. Regarding the skilled reaching test, the number of attempts, number of trials and the success rate decreased at 1 week post-repair. Subsequently, there was a brief rebound in performance observed at 2 weeks post-repair, followed by a continued decline in the number of attempts and trials. By 8 weeks post-repair, only the success rate had restored to levels similar to those observed at 4 weeks post-tear.
CONCLUSIONS: The skilled reaching test can detect functional deficiencies following rotator cuff tear and repair, while it requires high time and labour costs.
METHODS: In the control group, 8 male Sprague-Dawley rats received rotator cuff tear surgery without repair. In the rotator cuff repair group, 20 rats received rotator cuff repair at 4 weeks post rotator cuff tear. For the skilled reaching test, rats were trained to extend their forelimbs to fetch food pellets, and the number of trials, number of attempts and the success rate were recorded. The gait analysis and skilled reaching test were performed at baseline, 4 weeks post-tear, 1, 2, 4, and 8 weeks post-repair. The repeated measures analysis of variance was used to evaluate the effects of time on the shoulder function. The significance level was set at 0.05.
RESULTS: The skilled reaching test required 216 h to conduct, while the gait analysis took 44 h. In the rotator cuff repair group, gait performance significantly deteriorated at 1 week post-repair and restored to 4 weeks post-tear levels at 4 weeks post-repair. Regarding the skilled reaching test, the number of attempts, number of trials and the success rate decreased at 1 week post-repair. Subsequently, there was a brief rebound in performance observed at 2 weeks post-repair, followed by a continued decline in the number of attempts and trials. By 8 weeks post-repair, only the success rate had restored to levels similar to those observed at 4 weeks post-tear.
CONCLUSIONS: The skilled reaching test can detect functional deficiencies following rotator cuff tear and repair, while it requires high time and labour costs.
摘要：
背景：在肩袖损伤的临床和动物研究中，功能评估对于评估治疗结果至关重要。虽然步态分析通常用于评估肩袖撕裂的动物模型，它与人类患者的相关性较小，因为人类肩部通常是在非负重状态下评估的。本研究介绍了熟练的触角测试作为大鼠肩部功能评估工具，这允许评估没有承重。
方法：在对照组中，8只雄性Sprague-Dawley大鼠接受了无修复的肩袖撕裂手术。在肩袖修复组中，20只大鼠在肩袖撕裂后4周接受肩袖修复。对于熟练的触达测试，训练大鼠伸展前肢以获取食物颗粒，以及试验的数量，记录了尝试次数和成功率。在基线时进行步态分析和熟练到达测试，撕裂后4周，修复后1、2、4和8周。重复测量方差分析用于评估时间对肩关节功能的影响。显著性水平设定为0.05。
结果：熟练的测试需要216小时才能进行，而步态分析需要44小时。在肩袖修复组中，步态表现在修复后1周显著恶化,在修复后4周恢复至撕裂后4周水平。关于熟练的触达测试，尝试的次数,修复后1周的试验数量和成功率下降.随后，修复后2周观察到性能短暂反弹，随后，尝试和试验的数量持续下降。修复后8周，只有成功率恢复到与撕裂后4周相似的水平.
结论：熟练的伸手测试可以检测肩袖撕裂和修复后的功能缺陷，虽然它需要很高的时间和劳动力成本。
方法：在对照组中，8只雄性Sprague-Dawley大鼠接受了无修复的肩袖撕裂手术。在肩袖修复组中，20只大鼠在肩袖撕裂后4周接受肩袖修复。对于熟练的触达测试，训练大鼠伸展前肢以获取食物颗粒，以及试验的数量，记录了尝试次数和成功率。在基线时进行步态分析和熟练到达测试，撕裂后4周，修复后1、2、4和8周。重复测量方差分析用于评估时间对肩关节功能的影响。显著性水平设定为0.05。
结果：熟练的测试需要216小时才能进行，而步态分析需要44小时。在肩袖修复组中，步态表现在修复后1周显著恶化,在修复后4周恢复至撕裂后4周水平。关于熟练的触达测试，尝试的次数,修复后1周的试验数量和成功率下降.随后，修复后2周观察到性能短暂反弹，随后，尝试和试验的数量持续下降。修复后8周，只有成功率恢复到与撕裂后4周相似的水平.
结论：熟练的伸手测试可以检测肩袖撕裂和修复后的功能缺陷，虽然它需要很高的时间和劳动力成本。
