Abstract：
UNASSIGNED: Rotator cuff tears have been repaired using the transosseous method for decades. The direct suture (DS) technique has been widely used for rotator cuff tears; however, the retear rate is relatively high. Suture anchors are now used frequently for rotator cuff repair (RCR) in accordance with recent developments in materials. However, polyether ether ketone (PEEK) may still cause complications such as the formation of cysts and osteophytes. Some studies have developed the inlay suture (IS) technique for RCR.
UNASSIGNED: To compare how 3 different surgical techniques-namely, the DS, IS, and PEEK suture anchor (PSA)-affect tendon-bone healing after RCR. We hypothesized that the IS technique would lead to better tendon-to-bone healing and that the repaired structure would be similar to the normal enthesis.
UNASSIGNED: Controlled laboratory study.
UNASSIGNED: Acute infraspinatus tendon tears were created in 36 six-month-old male rabbits, which were divided into 3 groups based on the technique used for RCR: DS, IS, and PSA. Animals were euthanized at 6 and 12 weeks postoperatively and underwent a histological assessment and imaging. The expression of related proteins was demonstrated by immunohistochemistry and immunofluorescence staining. Mechanical properties were evaluated by biomechanical testing.
UNASSIGNED: At 12 weeks, regeneration of the enthesis was observed in the 3 groups. However, the DS group showed a lower type I collagen content than the PSA and IS groups, which was similar to the results for scleraxis. The DS group displayed a significantly inferior type II collagen expression and proteoglycan deposition after safranin O/fast green and sirius red staining. With regard to runt-related transcription factor 2 and alkaline phosphatase, the IS group showed upregulated expression levels compared with the other 2 groups.
UNASSIGNED: Compared with the DS technique, the PSA and IS techniques contributed to the improved maturation of tendons and fibrocartilage regeneration, while the IS technique particularly promoted osteogenesis at the enthesis.
UNASSIGNED: The IS and PSA techniques may be more beneficial for tendon-bone healing after RCR.
UNASSIGNED: To compare how 3 different surgical techniques-namely, the DS, IS, and PEEK suture anchor (PSA)-affect tendon-bone healing after RCR. We hypothesized that the IS technique would lead to better tendon-to-bone healing and that the repaired structure would be similar to the normal enthesis.
UNASSIGNED: Controlled laboratory study.
UNASSIGNED: Acute infraspinatus tendon tears were created in 36 six-month-old male rabbits, which were divided into 3 groups based on the technique used for RCR: DS, IS, and PSA. Animals were euthanized at 6 and 12 weeks postoperatively and underwent a histological assessment and imaging. The expression of related proteins was demonstrated by immunohistochemistry and immunofluorescence staining. Mechanical properties were evaluated by biomechanical testing.
UNASSIGNED: At 12 weeks, regeneration of the enthesis was observed in the 3 groups. However, the DS group showed a lower type I collagen content than the PSA and IS groups, which was similar to the results for scleraxis. The DS group displayed a significantly inferior type II collagen expression and proteoglycan deposition after safranin O/fast green and sirius red staining. With regard to runt-related transcription factor 2 and alkaline phosphatase, the IS group showed upregulated expression levels compared with the other 2 groups.
UNASSIGNED: Compared with the DS technique, the PSA and IS techniques contributed to the improved maturation of tendons and fibrocartilage regeneration, while the IS technique particularly promoted osteogenesis at the enthesis.
UNASSIGNED: The IS and PSA techniques may be more beneficial for tendon-bone healing after RCR.
摘要：
■使用透骨方法修复肩袖撕裂已有数十年。直接缝合（DS）技术已广泛用于肩袖撕裂；然而，再撕率相对较高。根据材料的最新发展，缝合锚现在经常用于肩袖修复（RCR）。然而,聚醚醚酮（PEEK）仍可能引起并发症，例如囊肿和骨赘的形成。一些研究已经开发了用于RCR的嵌体缝合(IS)技术。
■要比较3种不同的手术技术-即，DS,IS,和PEEK缝合锚（PSA）-影响RCR后的腱-骨愈合。我们假设IS技术将导致更好的腱-骨愈合，并且修复的结构将类似于正常的发生。
■对照实验室研究。
■在36只六个月大的雄性兔子中产生了急性冈下肌腱撕裂，根据用于RCR的技术将其分为3组：DS，IS,PSA。在术后6周和12周对动物实施安乐死,并进行组织学评估和成像。免疫组化和免疫荧光染色显示相关蛋白的表达。通过生物力学测试评估机械性能。
■在12周时，在3组中观察到了再生。然而,DS组I型胶原含量低于PSA和IS组,这与scleraxis的结果相似。在番红O/快速绿色和天狼星红染色后，DS组显示出明显较差的II型胶原蛋白表达和蛋白聚糖沉积。关于runt相关转录因子2和碱性磷酸酶，与其他两组相比，IS组的表达水平上调。
■与DS技术相比，PSA和IS技术有助于肌腱和纤维软骨再生的成熟，而IS技术特别促进了成骨。
■IS和PSA技术可能更有利于RCR后的腱-骨愈合。
■要比较3种不同的手术技术-即，DS,IS,和PEEK缝合锚（PSA）-影响RCR后的腱-骨愈合。我们假设IS技术将导致更好的腱-骨愈合，并且修复的结构将类似于正常的发生。
■对照实验室研究。
■在36只六个月大的雄性兔子中产生了急性冈下肌腱撕裂，根据用于RCR的技术将其分为3组：DS，IS,PSA。在术后6周和12周对动物实施安乐死,并进行组织学评估和成像。免疫组化和免疫荧光染色显示相关蛋白的表达。通过生物力学测试评估机械性能。
■在12周时，在3组中观察到了再生。然而,DS组I型胶原含量低于PSA和IS组,这与scleraxis的结果相似。在番红O/快速绿色和天狼星红染色后，DS组显示出明显较差的II型胶原蛋白表达和蛋白聚糖沉积。关于runt相关转录因子2和碱性磷酸酶，与其他两组相比，IS组的表达水平上调。
■与DS技术相比，PSA和IS技术有助于肌腱和纤维软骨再生的成熟，而IS技术特别促进了成骨。
■IS和PSA技术可能更有利于RCR后的腱-骨愈合。
