倒伏对水稻产量构成重大挑战,提示需要鉴定抗倒伏性状的优良等位基因,以改善栽培水稻品种。在这项研究中,检查了518个水稻种质的自然种群,以鉴定与植物高度(PH)相关的优良等位基因,茎直径(SD),阀杆反推(AT/S),和各种节间长度(第一(FirINL),秒(SecINL),第三(ThirINL),第四(ForINL),和第五(FifINL)节间长度)。通过在两种环境条件下进行关联映射,发现了与这些性状相关的262个SSR标记。表型评估显示品种之间存在显着差异,遗传多样性评估显示了各个种质的多态性。鉴定了PH的有利等位基因,SD,AT/S,和一到五个节间长度,与特定的等位基因显示相当大的影响。值得注意的等位基因包括6号染色体上的RM6811-160bp(其降低PH)和5号染色体上的RM161-145bp(其增加SD)。该研究确定了总共42个新的QTLs。具体来说,确定了七个pH值的QTL,四个是SD,五为AT/S,五个为Firinl,六个为Secinl,ThirinL的五个,六个为Forinl,还有四个给Fifinl.QTLsqAT/S-2,qPH2.1,qForINL2.1和qFifiINL的茎倒伏性状表现出最显着的表型变异(PVE)为3.99%。AT/S,PH,ForINL,FifINL具有5.31kPa的加性效应,5.42cm,4.27cm,和4.27厘米,分别,提供对八个不同的交叉组合的见解,以增强每个性状。这项研究表明,基于堆叠等位基因杂交优良亲本的潜力,有希望的改良水稻品种,具有增强的抗倒伏性,以满足市场需求。
Lodging poses a significant challenge to rice yield, prompting the need to identify elite alleles for lodging resistance traits to improve cultivated rice varieties. In this study, a natural population of 518 rice accessions was examined to identify elite alleles associated with plant height (PH), stem diameter (SD), stem anti-thrust (AT/S), and various internode lengths (first (FirINL), second (SecINL), third (ThirINL), fourth (ForINL), and fifth (FifINL) internode lengths). A total of 262 SSR markers linked to these traits were uncovered through association mapping in two environmental conditions. Phenotypic evaluations revealed striking differences among cultivars, and genetic diversity assessments showed polymorphisms across the accessions. Favorable alleles were identified for PH, SD, AT/S, and one to five internode lengths, with specific alleles displaying considerable effects. Noteworthy alleles include RM6811-160 bp on chromosome 6 (which reduces PH) and RM161-145 bp on chromosome 5 (which increases SD). The study identified a total of 42 novel
QTLs. Specifically, seven
QTLs were identified for PH, four for SD, five for AT/S, five for FirINL, six for SecINL, five for ThirINL, six for ForINL, and four for FifINL.
QTLs qAT/S-2, qPH2.1, qForINL2.1, and qFifINL exhibited the most significant phenotypic variance (PVE) of 3.99% for the stem lodging trait. AT/S, PH, ForINL, and FifINL had additive effects of 5.31 kPa, 5.42 cm, 4.27 cm, and 4.27 cm, respectively, offering insights into eight distinct cross-combinations for enhancing each trait. This research suggests the potential for crossbreeding superior parents based on stacked alleles, promising improved rice cultivars with enhanced lodging resistance to meet market demands.