根系结构是水稻抗旱性的决定因素,对水稻植株的水分和养分吸收起着至关重要的作用。解剖根系结构的遗传基础有助于提高水稻育种的抗逆性和产量。在这项研究中,共使用391个水稻(OryzasativaL.)种质对水稻的三个与根相关的性状进行了全基因组关联研究(GWAS),包括主根长度(MRL),平均根长(ARL),和总根数(TRN)。因此,鉴定出与3个性状显著相关的13个数量性状位点(QTLs)(qMRL1.1,qMRL1.2,qMRL3.1,qMRL3.2,qMRL3.3,qMRL4.1,qMRL7.1,qMRL8.1,qARL1.1,qARL9.1,qTRN9.2,qTRN11.1),其中qMRL3.2、qMRL4.1和qMRL8.1三个分别与OsGNOM1、OsARF12和qRL8.1重叠,还有十个是新颖的QTL。此外,我们还检测到影响根相关性状的上位性相互作用,并确定了19个相关的遗传相互作用。这些结果为克隆水稻根系结构的相应基因奠定了基础,为高产水稻品种的选育提供重要的基因组资源。
Root architecture is a determinant factor of drought resistance in rice and plays essential roles in the absorption of water and nutrients for the survival of rice plants. Dissection of the genetic basis for root structure can help to improve stress-resistance and grain yield in rice breeding. In this
study, a total of 391 rice (Oryz asativa L.) accessions were used to perform a genome-wide association
study (GWAS) on three root-related traits in rice, including main root length (MRL), average root length (ARL), and total root number (TRN). As a result, 13 quantitative trait loci (
QTLs) (qMRL1.1, qMRL1.2, qMRL3.1, qMRL3.2, qMRL3.3, qMRL4.1, qMRL7.1, qMRL8.1, qARL1.1, qARL9.1, qTRN9.1, qTRN9.2, and qTRN11.1) significantly associated with the three traits were identified, among which three (qMRL3.2, qMRL4.1 and qMRL8.1) were overlapped with OsGNOM1, OsARF12 and qRL8.1, respectively, and ten were novel
QTLs. Moreover, we also detected epistatic interactions affecting root-related traits and identified 19 related genetic interactions. These results lay a foundation for cloning the corresponding genes for rice root structure, as well as provide important genomic resources for breeding high yield rice varieties.