PDF(Pubmed)
Abstract：
UNASSIGNED: Breeding rice with drought tolerance for harsh environments is crucial for agricultural sustainability. Understanding the genetic underpinnings of drought tolerance is vital for developing resilient rice varieties. Genome-wide association studies (GWAS) have emerged as pivotal tools in unravelling the complex genetic architecture of traits like drought tolerance, capitalizing on the natural genetic diversity within rice germplasm collections.
UNASSIGNED: In this study, a comprehensive panel of 210 rice varieties was phenotyped over ten days in controlled conditions, subjected to simulated drought stress using 20% PEG 6000 in petri dishes. Throughout the stress period, crucial traits such as germination percentage (GP), germination rate index (GRI), mean germination time (MGT), and seedling percentage (SP) were meticulously monitored.
UNASSIGNED: The GWAS analysis uncovered a total of 38 QTLs associated with drought tolerance traits, including novel loci like qMGT-5.2, qSP-3, qSP7.2, and qGP-5.2. Additionally, RNA-seq analysis identified ten genes with significant expression differences under drought stress conditions. Notably, haplotype analysis pinpointed elite haplotypes in specific genes linked to heightened drought tolerance.
UNASSIGNED: Overall, this study underscores the importance of GWAS in validating known genes while unearthing novel loci to enrich the genetic resources for enhancing drought tolerance in rice breeding programs.
UNASSIGNED: In this study, a comprehensive panel of 210 rice varieties was phenotyped over ten days in controlled conditions, subjected to simulated drought stress using 20% PEG 6000 in petri dishes. Throughout the stress period, crucial traits such as germination percentage (GP), germination rate index (GRI), mean germination time (MGT), and seedling percentage (SP) were meticulously monitored.
UNASSIGNED: The GWAS analysis uncovered a total of 38 QTLs associated with drought tolerance traits, including novel loci like qMGT-5.2, qSP-3, qSP7.2, and qGP-5.2. Additionally, RNA-seq analysis identified ten genes with significant expression differences under drought stress conditions. Notably, haplotype analysis pinpointed elite haplotypes in specific genes linked to heightened drought tolerance.
UNASSIGNED: Overall, this study underscores the importance of GWAS in validating known genes while unearthing novel loci to enrich the genetic resources for enhancing drought tolerance in rice breeding programs.
摘要：
■培育对恶劣环境具有耐旱性的水稻对于农业可持续性至关重要。了解耐旱性的遗传基础对于开发抗旱性水稻品种至关重要。全基因组关联研究（GWAS）已成为揭示干旱耐受性等特征的复杂遗传结构的关键工具。利用水稻种质资源中的自然遗传多样性。
■在这项研究中，一个由210个水稻品种组成的综合小组在受控条件下进行了十天的表型鉴定，在培养皿中使用20%PEG6000进行模拟干旱胁迫。在整个压力时期，关键性状，如发芽率(GP)，发芽率指数（GRI），平均发芽时间（MGT），和幼苗百分比（SP）进行了精心监测。
■GWAS分析发现了总共38个与耐旱性相关的QTL，包括新的基因座，如qMGT-5.2，qSP-3，qSP7.2和qGP-5.2。此外,RNA-seq分析鉴定了10个在干旱胁迫条件下具有显著表达差异的基因。值得注意的是,单倍型分析确定了与耐旱性增强相关的特定基因中的精英单倍型。
■总的来说，这项研究强调了GWAS在验证已知基因，同时发掘新基因座以丰富遗传资源以增强水稻育种计划中的耐旱性方面的重要性。
■在这项研究中，一个由210个水稻品种组成的综合小组在受控条件下进行了十天的表型鉴定，在培养皿中使用20%PEG6000进行模拟干旱胁迫。在整个压力时期，关键性状，如发芽率(GP)，发芽率指数（GRI），平均发芽时间（MGT），和幼苗百分比（SP）进行了精心监测。
■GWAS分析发现了总共38个与耐旱性相关的QTL，包括新的基因座，如qMGT-5.2，qSP-3，qSP7.2和qGP-5.2。此外,RNA-seq分析鉴定了10个在干旱胁迫条件下具有显著表达差异的基因。值得注意的是,单倍型分析确定了与耐旱性增强相关的特定基因中的精英单倍型。
■总的来说，这项研究强调了GWAS在验证已知基因，同时发掘新基因座以丰富遗传资源以增强水稻育种计划中的耐旱性方面的重要性。
