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Abstract：
Grain shape is one of the most important factors that affects rice yield. Cloning novel grain shape genes and analyzing their genetic mechanisms are crucial for high yield breeding. In this study, a slender grain CSSL-Z485 with 3-segments substitution in the genetic background of Nipponbare was constructed in rice. Cytological analysis showed that the longer grain length of Z485 was related to the increase in glume cell numbers, while the narrower grain width was associated with the decrease in cell width. Three grain shape-related quantitative trait locus (QTLs), including qGL12, qGW12, and qRLW12, were identified through the F2 population constructed from a cross between Nipponbare and Z485. Furthermore, four single segment substitution lines (SSSLs, S1-S4) carrying the target QTLs were dissected from Z485 by MAS. Finally, three candidate genes of qGL12 for grain length and qGW12 for grain width located in S3 were confirmed by DNA sequencing, RT-qPCR, and protein structure prediction. Specifically, candidate gene 1 encodes a ubiquitin family protein, while candidate genes 2 and 3 encode zinc finger proteins. The results provide valuable germplasm resources for cloning novel grain shape genes and molecular breeding by design.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s11032-024-01480-x.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s11032-024-01480-x.
摘要：
籽粒形状是影响水稻产量的重要因素之一。克隆新的籽粒形状基因并分析其遗传机制对高产育种至关重要。在这项研究中,在水稻中构建了在Nipponbare遗传背景下具有3段替换的细长谷物CSSL-Z485。细胞学分析表明，Z485的粒长较长与颖细胞数量的增加有关，而较窄的晶粒宽度与细胞宽度的减小有关。三个籽粒形状相关的数量性状基因座（QTL），包括qGL12，qGW12和qRLW12，是通过从Nipponbare和Z485之间的杂交构建的F2种群确定的。此外，四个单段替换线(SSSL，通过MAS从Z485中解剖出带有目标QTL的S1-S4）。最后,通过DNA测序证实了位于S3中的qGL12的籽粒长度和qGW12的籽粒宽度的三个候选基因，RT-qPCR,和蛋白质结构预测。具体来说,候选基因1编码泛素家族蛋白，而候选基因2和3编码锌指蛋白。该结果为克隆新的粒形基因和设计分子育种提供了有价值的种质资源。
■在线版本包含补充材料，可在10.1007/s11032-024-01480-x获得。
■在线版本包含补充材料，可在10.1007/s11032-024-01480-x获得。
