PDF(Pubmed)
Abstract：
BACKGROUND: Developing and enriching genetic resources plays important role in the crop improvement. The flag leaf affects plant architecture and contributes to the grain yield of wheat (Triticum aestivum L.). The genetic improvement of flag leaf traits faces problems such as a limited genetic basis. Among the various genetic resources of wheat, Thinopyrum intermedium has been utilized as a valuable resource in genetic improvement due to its disease resistance, large spikes, large leaves, and multiple flowers. In this study, a recombinant inbred line (RIL) population was derived from common wheat Yannong15 and wheat-Th. intermedium introgression line SN304 was used to identify the quantitative trait loci (QTL) for flag leaf-related traits.
RESULTS: QTL mapping was performed for flag leaf length (FLL), flag leaf width (FLW) and flag leaf area (FLA). A total of 77 QTLs were detected, and among these, 51 QTLs with positive alleles were contributed by SN304. Fourteen major QTLs for flag leaf traits were detected on chromosomes 2B, 3B, 4B, and 2D. Additionally, 28 QTLs and 8 QTLs for flag leaf-related traits were detected in low-phosphorus and drought environments, respectively. Based on major QTLs of positive alleles from SN304, we identified a pair of double-ended anchor primers mapped on chromosome 2B and amplified a specific band of Th. intermedium in SN304. Moreover, there was a major colocated QTL on chromosome 2B, called QFll/Flw/Fla-2B, which was delimited to a physical interval of approximately 2.9 Mb and contained 20 candidate genes. Through gene sequence and expression analysis, four candidate genes associated with flag leaf formation and growth in the QTL interval were identified.
CONCLUSIONS: These results promote the fine mapping of QFll/Flw/Fla-2B, which have pleiotropic effects, and will facilitate the identification of candidate genes for flag leaf-related traits. Additionally, this work provides a theoretical basis for the application of Th. intermedium in wheat breeding.
RESULTS: QTL mapping was performed for flag leaf length (FLL), flag leaf width (FLW) and flag leaf area (FLA). A total of 77 QTLs were detected, and among these, 51 QTLs with positive alleles were contributed by SN304. Fourteen major QTLs for flag leaf traits were detected on chromosomes 2B, 3B, 4B, and 2D. Additionally, 28 QTLs and 8 QTLs for flag leaf-related traits were detected in low-phosphorus and drought environments, respectively. Based on major QTLs of positive alleles from SN304, we identified a pair of double-ended anchor primers mapped on chromosome 2B and amplified a specific band of Th. intermedium in SN304. Moreover, there was a major colocated QTL on chromosome 2B, called QFll/Flw/Fla-2B, which was delimited to a physical interval of approximately 2.9 Mb and contained 20 candidate genes. Through gene sequence and expression analysis, four candidate genes associated with flag leaf formation and growth in the QTL interval were identified.
CONCLUSIONS: These results promote the fine mapping of QFll/Flw/Fla-2B, which have pleiotropic effects, and will facilitate the identification of candidate genes for flag leaf-related traits. Additionally, this work provides a theoretical basis for the application of Th. intermedium in wheat breeding.
摘要：
背景：开发和丰富遗传资源对作物改良具有重要作用。旗叶影响植物结构，并有助于小麦（TriticumaestivumL.）的谷物产量。旗叶性状的遗传改良面临着遗传基础有限等问题。在小麦的各种遗传资源中,中间thinopyrum已被用作遗传改良的宝贵资源，由于其抗病性，大尖峰,大叶子,和多朵花。在这项研究中,重组自交系（RIL）种群来自普通小麦Yannong15和小麦-Th。中间渗入系SN304用于鉴定旗叶相关性状的数量性状基因座（QTL）。
结果：对旗叶长度（FLL）进行了QTL定位，旗叶宽度(FLW)和旗叶面积(FLA)。共检测到77个QTL,其中，SN304贡献了51个具有阳性等位基因的QTL。在2B号染色体上检测到十四个主要的旗叶性状QTL，3B,4B,和2D。此外,在低磷和干旱环境中检测到28个QTL和8个与旗叶相关的QTL，分别。基于SN304阳性等位基因的主要QTL，我们鉴定了一对定位在2B染色体上的双端锚定引物，并扩增了Th的特定条带。SN304中的中间。此外，2B染色体上有一个主要的同位QTL，称为QFll/Flw/Fla-2B，它的物理间隔约为2.9Mb，包含20个候选基因。通过基因序列和表达分析,确定了与QTL间隔中的旗叶形成和生长相关的四个候选基因。
结论：这些结果促进了QFll/Flw/Fla-2B的精细定位，具有多效性，并将有助于鉴定旗叶相关性状的候选基因。此外,为Th的应用提供了理论依据。小麦育种中的中间。
结果：对旗叶长度（FLL）进行了QTL定位，旗叶宽度(FLW)和旗叶面积(FLA)。共检测到77个QTL,其中，SN304贡献了51个具有阳性等位基因的QTL。在2B号染色体上检测到十四个主要的旗叶性状QTL，3B,4B,和2D。此外,在低磷和干旱环境中检测到28个QTL和8个与旗叶相关的QTL，分别。基于SN304阳性等位基因的主要QTL，我们鉴定了一对定位在2B染色体上的双端锚定引物，并扩增了Th的特定条带。SN304中的中间。此外，2B染色体上有一个主要的同位QTL，称为QFll/Flw/Fla-2B，它的物理间隔约为2.9Mb，包含20个候选基因。通过基因序列和表达分析,确定了与QTL间隔中的旗叶形成和生长相关的四个候选基因。
结论：这些结果促进了QFll/Flw/Fla-2B的精细定位，具有多效性，并将有助于鉴定旗叶相关性状的候选基因。此外,为Th的应用提供了理论依据。小麦育种中的中间。
