RESULTS: QTL mapping was performed for flag leaf length (FLL), flag leaf width (FLW) and flag leaf area (FLA). A total of 77 QTLs were detected, and among these, 51 QTLs with positive alleles were contributed by SN304. Fourteen major QTLs for flag leaf traits were detected on chromosomes 2B, 3B, 4B, and 2D. Additionally, 28 QTLs and 8 QTLs for flag leaf-related traits were detected in low-phosphorus and drought environments, respectively. Based on major QTLs of positive alleles from SN304, we identified a pair of double-ended anchor primers mapped on chromosome 2B and amplified a specific band of Th. intermedium in SN304. Moreover, there was a major colocated QTL on chromosome 2B, called QFll/Flw/Fla-2B, which was delimited to a physical interval of approximately 2.9 Mb and contained 20 candidate genes. Through gene sequence and expression analysis, four candidate genes associated with flag leaf formation and growth in the QTL interval were identified.
CONCLUSIONS: These results promote the fine mapping of QFll/Flw/Fla-2B, which have pleiotropic effects, and will facilitate the identification of candidate genes for flag leaf-related traits. Additionally, this work provides a theoretical basis for the application of Th. intermedium in wheat breeding.
结果:对旗叶长度(FLL)进行了QTL定位,旗叶宽度(FLW)和旗叶面积(FLA)。共检测到77个QTL,其中,SN304贡献了51个具有阳性等位基因的QTL。在2B号染色体上检测到十四个主要的旗叶性状QTL,3B,4B,和2D。此外,在低磷和干旱环境中检测到28个QTL和8个与旗叶相关的QTL,分别。基于SN304阳性等位基因的主要QTL,我们鉴定了一对定位在2B染色体上的双端锚定引物,并扩增了Th的特定条带。SN304中的中间。此外,2B染色体上有一个主要的同位QTL,称为QFll/Flw/Fla-2B,它的物理间隔约为2.9Mb,包含20个候选基因。通过基因序列和表达分析,确定了与QTL间隔中的旗叶形成和生长相关的四个候选基因。
结论:这些结果促进了QFll/Flw/Fla-2B的精细定位,具有多效性,并将有助于鉴定旗叶相关性状的候选基因。此外,为Th的应用提供了理论依据。小麦育种中的中间。