霍山石斛提取物,属于兰科的传统中药和食品同源植物,以前报道有降血糖和抗氧化作用。在这项研究中,多糖(DHP)和非多糖(NDHP)成分的直接作用,使用体外发酵方法,将其水提取物(DHWE)与二甲双胍(一种抗糖尿病药物)对链脲佐菌素诱导的1型糖尿病(T1D)大鼠的肠道微生物群(从粪便菌群中收集)进行了比较。结果显示DHWE,DHP,NDHP降低了pH,增加了发酵液中细菌的增殖和短链脂肪酸(SCFA)含量。DHWE,DHP,NDHP和二甲双胍促进乙酸和丙酸的产生,乙酸,丙酸和丁酸,和丙酸,分别。DHWE,DHP,NDHP降低了变形杆菌(优势致病菌)的丰度,并增加了Firmicutes(优势有益肠道细菌)的丰度。NDHP还降低了拟杆菌(有益和条件致病)的丰度。二甲双胍增加了变形杆菌的丰度,并降低了厚壁菌和拟杆菌的丰度。在属一级,NDHP促进Megamonas和Megasphaera的增殖并减少有害细菌(例如,克雷伯菌属),和DHP增加了Prevotellaceae的丰度(机会性,通常无害)。相比之下,二甲双胍增加了有害细菌的丰度(例如,柠檬酸杆菌)和减少有益细菌的丰度(例如,螺旋体)。我们的研究表明DHWE,DHP,与二甲双胍相比,NDHP对体外T1D大鼠的肠道菌群可能更有益。
The extract of Dendrobium huoshanense, a traditional Chinese medicinal and food homologous plant belonging to the family Orchidaceae, was previously reported to have hypoglycemic and antioxidant effects. In this study, the direct effects of polysaccharide (DHP) and non-polysaccharide (NDHP) components of D. huoshanense, as well as its water extract (DHWE) were compared with that of metformin (an antidiabetic drug) on the gut microbiota (collected from fecal flora) of rats with streptozotocin-induced type 1 diabetes (T1D) using an in vitro fermentation method. The results showed that DHWE, DHP, and NDHP reduced pH and increased bacterial proliferation and short-chain fatty acid (SCFA) content in fermentation broth. DHWE, DHP, NDHP and metformin promoted the production of acetic and propionic acid, acetic acid, propionic acid and butyric acid, and propionic acid, respectively. DHWE, DHP, and NDHP reduced the abundance of Proteobacteria (subdominant pathogenic bacteria) and increased the abundance of Firmicutes (dominant beneficial gut bacteria). NDHP also reduced the abundance of Bacteroidetes (beneficial and conditional pathogenic). Metformin increased the abundance of Proteobacteria and reduced the abundance of Firmicutes and Bacteroidetes. At the genus level, NDHP promoted the proliferation of Megamonas and Megasphaera and decreased harmful bacteria (e.g., Klebsiella), and DHP increased the abundance of Prevotellaceae (opportunistic and usually harmless). By contrast, metformin increased the abundance of harmful bacteria (e.g., Citrobacter) and reduced the abundance of beneficial bacteria (e.g., Oscillospira). Our study indicates that DHWE, DHP, and NDHP are potentially more beneficial than metformin on the gut microbiota of T1D rats in vitro.