PDF(Pubmed)
Abstract：
During the adaptive evolution of animals, the host and its gut microbiota co-adapt to different elevations. Currently, there are few reports on the rumen microbiota-hepato-intestinal axis of Tibetan sheep at different altitudes. Therefore, the purpose of this study was to explore the regulatory effect of rumen microorganism-volatile fatty acids (VFAs)-VFAs transporter gene interactions on the key enzymes and genes related to gluconeogenesis in Tibetan sheep. The rumen fermentation parameters, rumen microbial densities, liver gluconeogenesis activity and related genes were determined and analyzed using gas chromatography, RT-qPCR and other research methods. Correlation analysis revealed a reciprocal relationship among rumen microflora-VFAs-hepatic gluconeogenesis in Tibetan sheep at different altitudes. Among the microbiota, Ruminococcus flavefaciens (R. flavefaciens), Ruminococcus albus (R. albus), Fibrobactersuccinogenes and Ruminobacter amylophilus (R. amylophilus) were significantly correlated with propionic acid (p < 0.05), while propionic acid was significantly correlated with the transport genes monocarboxylate transporter 4 (MCT4) and anion exchanger 2 (AE2) (p < 0.05). Propionic acid was significantly correlated with key enzymes such as pyruvate carboxylase, phosphoenolpyruvic acid carboxylase and glucose (Glu) in the gluconeogenesis pathway (p < 0.05). Additionally, the expressions of these genes were significantly correlated with those of the related genes, namely, forkhead box protein O1 (FOXO1) and mitochondrial phosphoenolpyruvate carboxykinase 2 (PCK2) (p < 0.05). The results showed that rumen microbiota densities differed at different altitudes, and the metabolically produced VFA contents differed, which led to adaptive changes in the key enzyme activities of gluconeogenesis and the expressions of related genes.
摘要：
在动物的适应性进化过程中,宿主及其肠道微生物群共同适应不同的海拔。目前,关于不同海拔高度藏绵羊瘤胃微生物-肝肠轴的报道很少。因此,本研究旨在探讨瘤胃微生物-挥发性脂肪酸(VFAs)-VFAs转运体基因相互作用对藏羊糖异生关键酶及相关基因的调控作用。瘤胃发酵参数，瘤胃微生物密度，肝脏糖异生活性和相关基因的测定和分析使用气相色谱法，RT-qPCR等研讨办法。相关分析表明，不同海拔地区藏绵羊瘤胃微生物区系-VFAs-肝糖异生之间存在相互关系。在微生物群中，黄色反刍动物(R.flavefaciens)，白乳球菌(R.albus),产琥珀酸纤维杆菌和嗜淀粉反刍杆菌（R.嗜淀粉样蛋白)与丙酸显著相关(p<0.05)，而丙酸与转运基因单羧酸转运蛋白4（MCT4）和阴离子交换蛋白2（AE2）显着相关（p<0.05）。丙酸与丙酮酸羧化酶等关键酶显著相关,磷酸烯醇式丙酮酸羧化酶和葡萄糖(Glu)参与糖异生途径(p<0.05)。此外,这些基因的表达与相关基因的表达显着相关，即,叉头盒蛋白O1（FOXO1）和线粒体磷酸烯醇丙酮酸羧激酶2（PCK2）（p<0.05）。结果表明,不同海拔高度瘤胃微生物密度存在差异,代谢产生的VFA含量不同，导致糖异生关键酶活性和相关基因表达发生适应性变化。
