■脓毒症是一种高度混合的疾病,可影响具有多种感染源条件的患者,并可导致多器官衰竭,宿主免疫反应失调。
■确定革兰氏阴性菌引起的败血症患者的炎症生物标志物,并比较它们在败血症早期检测中的作用。
■这项横断面研究是针对苏莱曼尼亚不同医院重症监护病房收治的脓毒症患者,伊拉克,2021年5月至12月。根据序贯器官衰竭评估评分对败血症的初步诊断,将患者(n=147)纳入本研究。从患者身上采集血样以调查白细胞,炎性生物标志物(pentraxin-3,降钙素原,肾上腺髓质素,脂多糖结合蛋白,白细胞介素-17A,乳酸脱氢酶,和C创意蛋白),血培养,抗生素药敏试验,和凝血生物标志物(凝血酶原时间,活化部分凝血活酶时间,和国际标准化比率)。然后,通过筛选和联合圆盘试验,对分离的革兰氏阴性菌进行了超广谱β-内酰胺酶生产试验。
■共有51.7%的样本是不同革兰氏阴性菌的血培养阳性,铜绿假单胞菌(51.95%)是较分离的细菌。在不同年龄组中,男性和女性均以1.23:1的比例受到败血症的影响。根据抗生素谱,超广谱β-内酰胺酶估计为77.2%,并且使用两个双盘协同试验来降低速率。这通过组合盘试验以41.35%的比率证实。最普遍的生物标志物是降钙素原(88.16%),肾上腺髓质素(84.21%),pentraxin-3(22.37%),和脂多糖结合蛋白(11.84%)。
脓毒症是一种危及生命的疾病,可以通过几种血液生物标志物如降钙素原早期诊断,肾上腺髓质素,和pentraxin-3与标准的血培养技术相结合,以改善患者的预后。
UNASSIGNED: Sepsis is a highly mixed ailment that affects patients with numerous conditions of infectious sources and can lead to multi-organ failure with dysregulated host immune response.
UNASSIGNED: To determine inflammatory biomarkers in patients with sepsis caused by Gram-negative bacteria and compare their role in the early detection of sepsis.
UNASSIGNED: This cross-sectional
study was conducted on patients with sepsis admitted to the intensive care unit at different hospitals in Sulaimaniyah, Iraq, from May to December 2021. Patients (n=147) were enrolled in this
study according to the primary diagnosis of sepsis by Sequential Organ Failure Assessment scores. Blood samples were taken from patients to investigate white blood cells, inflammatory biomarkers (pentraxin-3, procalcitonin, adrenomedullin, lipopolysaccharide binding protein, interleukin-17A, lactate dehydrogenase, and C-creative protein), blood culture, antibiotic susceptibility test, and coagulation biomarkers (Prothrombin time, activated partial thromboplastin time, and international normalized ratio). Then, isolated Gram-negative bacteria were tested for extended-spectrum β-lactamase enzymes production by screening and combined disc tests.
UNASSIGNED: A total of 51.7% samples were blood culture positive for different Gram-negative bacteria, and P. aeruginosa (51.95%) was a more isolated bacterium. Both males and females were affected by sepsis in a ratio of 1.23:1 with different age groups. Extended-spectrum β-lactamase was estimated to be 77.2% by antibiotic profile, and the rate decreased using two double-disc synergy tests. This was confirmed by combined disc test at a rate of 41.35%. The most prevalent biomarkers were procalcitonin (88.16%), adrenomedullin (84.21%), pentraxin-3 (22.37%), and lipopolysaccharide binding protein (11.84%).
UNASSIGNED: Sepsis is a life-threatening condition that can be diagnosed early by several blood biomarkers such as procalcitonin, adrenomedullin, and pentraxin-3 combined with a standard blood culture technique to improve the patient outcome.