背景:在Li-Fraumeni综合征(LFS)患者中经常报道胚系TP53突变,导致各种恶性肿瘤的倾向。种系TP53突变以外的突变也可导致LFS相关恶性肿瘤,但他们的细节仍不清楚。我们描述了LFS患者独特的脂肪肉瘤中的新型c-myc扩增。
方法:一名患有LFS的女性患者在30岁时两次发展为乳腺癌;两者均为具有HER2扩增的浸润性导管癌。计算机断层扫描显示前纵隔肿块,手术切除了.组织学分析显示对应于粘液样脂肪肉瘤的三种不同病变,多形性脂肪肉瘤,和分化良好的脂肪肉瘤样病变。荧光原位杂交(FISH)分析未检测到MDM2扩增,Rb1删除,分解EWS的信号,FUS,DDIT3,或c-myc,或者c-myc-IGH融合信号,但它确实检测到更多的c-myc信号。进一步的FISH分析和全面的基因组分析显示c-myc扩增。我们考虑了两种鉴别诊断,缺乏MDM2扩增的去分化脂肪肉瘤和粘液样多形性脂肪肉瘤(MPLPS),并确定这种情况最有可能是MPLPS。然而,无法明确诊断,因为无法明确区分病例与脂肪肉瘤.
结论:先前的研究表明,在各种脂肪肉瘤中无法检测到c-myc扩增,但是目前独特的脂肪肉瘤显示c-myc扩增,因此,c-myc扩增可能表明目前的脂肪肉瘤是LFS相关肿瘤。本病例通过扩大其组织病理学和遗传多样性,进一步阐明了MPLPS和LFS相关脂肪肉瘤的病理特征。
BACKGROUND: Germline TP53 mutations have been frequently reported in patients with Li-Fraumeni syndrome (LFS), resulting in a predisposition to various malignancies. Mutations other than germline TP53 mutations can also cause LFS-associated malignancies, but their details remain unclear. We describe a novel c-myc amplification in a unique liposarcoma in a patient with LFS.
METHODS: A female patient with LFS developed breast cancer twice at the age of thirty; both were invasive ductal carcinomas harboring HER2 amplifications. Computed tomography revealed an anterior mediastinal mass, which was surgically resected. Histological analysis revealed three different lesions corresponding to myxoid liposarcoma-, pleomorphic liposarcoma-, and well-differentiated liposarcoma-like lesions. Fluorescence in-situ hybridization (FISH) analysis did not detect MDM2 amplification, Rb1 deletion, break apart signals of EWS, FUS, DDIT3, or c-myc, or c-myc-IGH fusion signals, but it did detect more c-myc signals. Further FISH analysis and comprehensive genomic profiling revealed c-myc amplification. We considered two differential diagnoses, dedifferentiated liposarcoma lacking MDM2 amplification and myxoid pleomorphic liposarcoma (MPLPS), and determined that this
case is most likely MPLPS. However, definite diagnosis could not be made because a clear-cut differentiation of the
case from liposarcomas was not possible.
CONCLUSIONS: A previous study demonstrated that c-myc amplification could not be detected in various liposarcomas, but the present unique liposarcoma showed c-myc amplification, so the c-myc amplification may indicate that the present liposarcoma is an LFS-related tumor. The present
case further clarifies the pathological features of MPLPS and LFS-related liposarcomas by broadening their histopathological and genetic diversities.