■银(I)-二氨基氟化物(SDF)和银(I)-氟化物(SF)络合物已经成功地用于龋齿的阻止多年。然而,迄今为止,很少有研究报告这些药物的分子结构组成和溶液状态[通常作为高浓度的38%(w/v)溶液应用]。这里,我们探索了市售SDF和SF产品的溶液状态和化学成分,其次,研究了这些产品与完整的人全口唾液上清液(WMSS)中存在的生物分子的多组分相互作用。
■高分辨率19FNMR分析用于探索SDF和SF产品解决方案,并确定WMSS氟化物(F-)浓度,而氨(NH3)释放形式SDF通过1HNMR光谱追踪。采用SEM和薄膜FTIR-ATR分析来探索顺序生成的AgCl沉积物和发色Ag/AgCl纳米颗粒(CSNP)的原子和分子组成;后者的时间依赖性生成是通过分光光度法进行的。
■SF水溶液的19FNMR光谱包含非常宽的F-信号(Δv1/270Hz),证明其溶剂化的F-含量在NMR时间尺度上与Ag(I)快速交换,但是自卫队的人有更强烈的共鸣,类似于“自由”F-(4Hz)。此外,进一步的NMR结果表明,流行的SDF产物含有高摩尔过量的F-和NH3。用SDF和SF处理WMSS产生灰白色沉淀,在23°C下缓慢发展成CSNP;SEM显示该材料中银和氯化物的含量很高(原子含量比为ca.1:1)。FTIR-ATR分析发现,形成的CSNP含有一系列唾液生物分子,其似乎包封Ag/AgCl核(还发现显著的硫氰酸盐含量)。总之,获得的核磁共振结果表明,SF,但不是SDF,产品解决方案具有在其“自由”形式和Ag(I)结合形式之间快速交换F-,并且SDF包含大量过量的F-及其NH3配体。这些复合物与WMSS生物分子的相互作用依次产生了表征的AgCl沉积物和CSNP。
■鉴于其众所周知的杀微生物和防龋性能,观察到的涉及唾液催化的CSNP的自体生物构建具有重要的治疗意义。
UNASSIGNED: Silver(I)-diammine fluoride (SDF) and silver(I)-fluoride (SF) complexes have been successfully employed for the arrest of dental caries for many years. However, to date there are very few studies available reporting on the molecular structural compositional and solution status of these agents [typically applied as highly-concentrated 38% (w/v) solutions]. Here, we explored the solution status and chemical constitution of commercially-available SDF and SF products, and secondly investigated the multicomponent interplay of these products with biomolecules present in intact human whole-mouth salivary supernatants (WMSSs) in vitro.
UNASSIGNED: High-resolution 19F NMR analysis was employed to explore SDF and SF product solutions, and to determine WMSS fluoride (F-) concentrations, whereas ammonia (NH3) release form SDF was tracked by 1H NMR spectroscopy. SEM and thin-film FTIR-ATR analyses were employed to explore the atomic and molecular compositions of sequentially-generated AgCl deposits and chromophoric Ag/AgCl nanoparticles (CSNPs); the time-dependent generation of the latter was followed spectrophotometrically.
UNASSIGNED: 19F NMR spectra of aqueous SF solutions contained a very broad F- signal (Δv1/2 70 Hz), demonstrating that much of its solvated F- content was rapidly exchanging with Ag(I) on the NMR timescale, but those of SDF had a much sharper resonance, similar to that of \"free\" F- (4 Hz). Moreover, further NMR results revealed that a popular SDF product contained high molar excesses of both F- and NH3. Treatment of WMSSs with SDF and SF generated an off-white precipitate, which slowly developed into CSNPs at 23°C; SEM demonstrated high contents of both silver and chloride in this material (ca.1:1 atomic content ratio). FTIR-ATR analysis found that the CSNPs formed contained a range of salivary biomolecules, which appear to encapsulate the Ag/AgCl core (significant thiocyanate contents were also found). In conclusion, NMR results acquired demonstrated that SF, but not SDF, product solutions feature rapidly-exchanging F - between its \"free\" and Ag(I)-bound forms, and that SDF contains large excesses of both F- and its NH3 ligands. Characterised AgCl deposits and CSNPs were sequentially produced from the interactions of these complexes with WMSS biomolecules.
UNASSIGNED: In view of their well-known microbicidal and cariostatic properties, the observed autobioconstruction of CSNPs involving salivary catalysis is of much therapeutic significance.