目的:我们的孟德尔随机化(MR)分析集中于调查重度抑郁症(MDD)之间的双向关系,焦虑和压力相关障碍(ASRD),龋齿和牙周炎。
方法:我们使用了两项研究的汇总统计数据:一项MDD全基因组关联研究(GWAS),包括135,458例,344,901例对照,以及一项基于12,655名ASRD个体和19,225名来自丹麦的对照的Lundbeck基金会整合精神病学研究倡议(iPSYCH)GWAS。龋齿和牙周炎的GWAS基于牙科终点中的基因-生活方式相互作用(GLIDE)联盟。我们采用了不同的MR方法,例如逆方差加权(IVW),MR-Egger,加权中位数,和MR-PRESSO,计算因果效应。
结果:单变量MR分析显示,ASRD可能与衰变有关,失踪,和填充的牙齿表面(DMFS)(β=0.056;95%CI:0.009,0.103;p=0.018)。提示牙周炎与ASRD风险增加有因果关系(OR=1.143,95%CI:1.008,1.298;p=0.038)。根据多变量MR分析,在MDD和ASRD之间以及龋齿和牙周炎之间没有检测到显着的关联,反之亦然。
结论:ASRD显示出与DMFS的潜在关联,根据单变量MR分析发现牙周炎可能影响ASRD。然而,MDD之间没有发现显著关联,ASRD,龋齿,或牙周炎后调整吸烟状况和教育水平。因此,需要更强大的遗传工具来验证和加强我们的发现。
OBJECTIVE: Our Mendelian randomization (MR) analysis focused on investigating the bidirectional relationships between major depressive disorder (MDD), anxiety and stress-related disorder (ASRD), and dental caries as well as periodontitis.
METHODS: We used summary statistics from two studies: an MDD genome-wide association study (GWAS) including 135,458 cases with 344,901 controls and a Lundbeck Foundation Initiative for Integrative Psychiatric Research (iPSYCH) GWAS based on 12,655 ASRD individuals and 19,225 controls from Denmark. GWASs on dental caries and periodontitis were based on the Gene-Lifestyle Interactions in Dental Endpoints (GLIDE) consortium. We employed different MR approaches, such as inverse-variance weighted (IVW), MR-Egger, weighted median, and MR-PRESSO, to calculate causal effects.
RESULTS: Single-variable MR analysis revealed that ASRD was potentially significantly associated with decayed, missing, and filled tooth surfaces (DMFS) (β = 0.056; 95 % CI: 0.009, 0.103; p = 0.018). Periodontitis was suggested to be causally related to increased ASRD risk (OR = 1.143, 95 % CI: 1.008, 1.298; p = 0.038). According to the multivariable MR analysis, no significant associations were detected between MDD and ASRD with dental caries and periodontitis, and vice versa.
CONCLUSIONS: ASRD demonstrated a potential association with DMFS, and periodontitis was found to potentially impact ASRD according to single-variable MR analysis. Nevertheless, no significant associations were identified between MDD, ASRD, dental caries, or periodontitis after adjusting for smoking status and education level. Hence, more robust genetic instruments are required to validate and reinforce our findings.