背景:丹参。(迷迭香)被认为是经济上重要的观赏植物和药用植物,广泛用于烹饪和治疗多种疾病。然而,在迷迭香中,在分子水平上合成基于次级代谢产物的生物活性化合物背后的过程尚未完全探索.
结果:我们在IlluminaHiSeqTMX10平台上对来自叶片和茎组织的合并样品进行了转录组测序。转录组学分析导致产生29,523,608个原始读数,接下来是数据预处理,生成了23,208,592个干净的读数,迷迭香从头组装获得了166,849个基因。其中,近75.1%的unigenes,即,针对非冗余蛋白质数据库解释了28,757。基于基因本体的注释将它们分为3个主类和55个亚类,和直系同源基因注释簇将它们分为23个功能类别。基于基因和基因组数据库的途径分析的京都百科全书证实了在183个生化途径中涉及13,402个单基因,在这些基因中,1,186涉及17种次级代谢物产生途径。从转录组数据库中鉴定了几种涉及产生芳香族氨基酸和苯丙烷类的关键酶。在确定的48个编码单基因的转录因子家族中,bHLH,MYB,WRKYs,NAC,C2H2,C3H,和ERF参与类黄酮和其他次生代谢产物的生物合成。
结论:系统发育分析揭示了迷迭香苯丙素途径基因与唇形科其他成员之间的进化关系。我们的工作揭示了迷迭香植物中苯丙素生物合成及其调控背后的新分子机制。
BACKGROUND: The Salvia rosmarinus spenn. (
rosemary) is considered an economically important ornamental and medicinal plant and is widely utilized in culinary and for treating several diseases. However, the procedure behind synthesizing secondary metabolites-based bioactive compounds at the molecular level in S. rosmarinus is not explored completely.
RESULTS: We performed transcriptomic sequencing of the pooled sample from leaf and stem tissues on the Illumina HiSeqTM X10 platform. The transcriptomics analysis led to the generation of 29,523,608 raw reads, followed by data pre-processing which generated 23,208,592 clean reads, and de novo assembly of S. rosmarinus obtained 166,849 unigenes. Among them, nearly 75.1% of unigenes i.e., 28,757 were interpreted against a non-redundant protein database. The gene ontology-based annotation classified them into 3 main categories and 55 sub-categories, and clusters of orthologous genes annotation categorized them into 23 functional categories. The Kyoto Encyclopedia of Genes and Genomes database-based pathway analysis confirmed the involvement of 13,402 unigenes in 183 biochemical pathways, among these unigenes, 1,186 are involved in the 17 secondary metabolite production pathways. Several key enzymes involved in producing aromatic amino acids and phenylpropanoids were identified from the transcriptome database. Among the identified 48 families of transcription factors from coding unigenes, bHLH, MYB, WRKYs, NAC, C2H2, C3H, and ERF are involved in flavonoids and other secondary metabolites biosynthesis.
CONCLUSIONS: The phylogenetic analysis revealed the evolutionary relationship between the phenylpropanoid pathway genes of
rosemary with other members of Lamiaceae. Our work reveals a new molecular mechanism behind the biosynthesis of phenylpropanoids and their regulation in
rosemary plants.