METHODS: Carvacrol and rosemary essential oils were obtained, and nano emulsions were formulated. NE were characterized using TEM for charge and size distribution. The A375 human melanoma cell line was cultured and propagated then IC50 of prepared NE was added. Assessment of cell cytotoxicity, effect on angiogenesis and apoptosis were tested.
RESULTS: After synthesis and characterization, both carvacrol nano emulsion (CNE) and rosemary nano emulsion (RNE) were capable of inhibiting melanoma cell line viability, angiogenesis and they enhanced the expression of caspase-3 proapoptotic marker.
CONCLUSIONS: Rosemary and carvacrol extract nano emulsions could be a new revolutionary agent in human melanoma therapy and these formulations can be applied locally.
方法:获得香芹酚和迷迭香精油,和纳米乳液被配制。使用TEM表征NE的电荷和尺寸分布。培养和增殖A375人黑素瘤细胞系,然后加入制备的NE的IC50。细胞毒性评估,对血管生成和细胞凋亡的影响进行了测试。
结果:合成和表征后,香芹酚纳米乳剂(CNE)和迷迭香纳米乳剂(RNE)均能抑制黑素瘤细胞系的活力,血管生成和它们增强caspase-3促凋亡标志物的表达。
结论:迷迭香和香芹酚提取物纳米乳液可能是人类黑色素瘤治疗的新的革命性药物,这些制剂可以局部应用。