PDF(Pubmed)
Abstract：
BACKGROUND: Cancer is a challenge for either the patient or the healthcare manager. Treatment protocols based on chemotherapy or radiotherapy, or both are interfering with the patient\'s life making him suffer rather than being alleviated. This burden pushed the scientists to search for new regimens that may help ameliorate patient as well as doctor inconvenience. Benefits of plant extracts as medical substitutes in cancer management have been proved. New nano formulated drug delivery systems may help overcoming remedy regimens barriers and obstacles. The present research topic aims to evaluate the anticancer power of two plant extracts in nano emulsion formulation on human melanoma cell line.
METHODS: Carvacrol and rosemary essential oils were obtained, and nano emulsions were formulated. NE were characterized using TEM for charge and size distribution. The A375 human melanoma cell line was cultured and propagated then IC50 of prepared NE was added. Assessment of cell cytotoxicity, effect on angiogenesis and apoptosis were tested.
RESULTS: After synthesis and characterization, both carvacrol nano emulsion (CNE) and rosemary nano emulsion (RNE) were capable of inhibiting melanoma cell line viability, angiogenesis and they enhanced the expression of caspase-3 proapoptotic marker.
CONCLUSIONS: Rosemary and carvacrol extract nano emulsions could be a new revolutionary agent in human melanoma therapy and these formulations can be applied locally.
METHODS: Carvacrol and rosemary essential oils were obtained, and nano emulsions were formulated. NE were characterized using TEM for charge and size distribution. The A375 human melanoma cell line was cultured and propagated then IC50 of prepared NE was added. Assessment of cell cytotoxicity, effect on angiogenesis and apoptosis were tested.
RESULTS: After synthesis and characterization, both carvacrol nano emulsion (CNE) and rosemary nano emulsion (RNE) were capable of inhibiting melanoma cell line viability, angiogenesis and they enhanced the expression of caspase-3 proapoptotic marker.
CONCLUSIONS: Rosemary and carvacrol extract nano emulsions could be a new revolutionary agent in human melanoma therapy and these formulations can be applied locally.
摘要：
背景：癌症对患者或医疗保健管理者都是一个挑战。基于化疗或放疗的治疗方案，或者两者都在干扰病人的生活，使他受苦而不是得到缓解。这种负担促使科学家们寻找新的治疗方案，以帮助改善患者和医生的不便。已经证明了植物提取物作为癌症治疗中的医疗替代品的益处。新的纳米配方药物递送系统可能有助于克服治疗方案的障碍和障碍。本课题旨在评价两种植物提取物在纳米乳液制剂中对人黑色素瘤细胞系的抗癌能力。
方法：获得香芹酚和迷迭香精油，和纳米乳液被配制。使用TEM表征NE的电荷和尺寸分布。培养和增殖A375人黑素瘤细胞系,然后加入制备的NE的IC50。细胞毒性评估，对血管生成和细胞凋亡的影响进行了测试。
结果：合成和表征后，香芹酚纳米乳剂(CNE)和迷迭香纳米乳剂(RNE)均能抑制黑素瘤细胞系的活力,血管生成和它们增强caspase-3促凋亡标志物的表达。
结论：迷迭香和香芹酚提取物纳米乳液可能是人类黑色素瘤治疗的新的革命性药物，这些制剂可以局部应用。
方法：获得香芹酚和迷迭香精油，和纳米乳液被配制。使用TEM表征NE的电荷和尺寸分布。培养和增殖A375人黑素瘤细胞系,然后加入制备的NE的IC50。细胞毒性评估，对血管生成和细胞凋亡的影响进行了测试。
结果：合成和表征后，香芹酚纳米乳剂(CNE)和迷迭香纳米乳剂(RNE)均能抑制黑素瘤细胞系的活力,血管生成和它们增强caspase-3促凋亡标志物的表达。
结论：迷迭香和香芹酚提取物纳米乳液可能是人类黑色素瘤治疗的新的革命性药物，这些制剂可以局部应用。
