背景:已知类肝素,多硫酸粘多糖,有效改善皮肤粗糙和促进血液循环作为疾病区域的药物。然而,类肝素的分子量超过5000,不能穿透健康的角质层。
目的:我们测试了分子量小于2000的硫酸化寡糖对人体皮肤屏障功能和保湿功能的功效。
方法:我们测量了局部施用硫酸化寡糖后培养3天的三维人类表皮模型的经皮失水(TEWL),然后观察对TEWL抑制的影响。角质层中参与细胞间脂质运输和储存的蛋白质的mRNA水平,使用RT-qPCR测量和保湿性。
结果:ATP结合盒亚家族A成员12(ABCA12)的mRNA水平增加,将脂质输送到颗粒层中,已确认。丝聚蛋白(FLG)的mRNA水平也增加,参与天然保湿因子的产生,以及caspase-14,calpain-1和博来霉素水解酶,这与FLG的降解有关。抗体染色证实,将海藻糖硫酸钠应用于3D模型皮肤导致更多的ABCA12,神经酰胺,转谷氨酰胺酶1和FLG比对照组。在一个随机的,安慰剂对照,双盲研究,角质层含水量低的参与者将含有海藻糖硫酸钠的乳液和乳液涂在脸上4周。海藻糖硫酸钠降低了TEWL并增加了角质层的含水量。
结论:这些结果表明,含有海藻糖硫酸钠的化妆品通过增加屏障因子和保湿因子而作用于表皮,从而改善皮肤干燥。
BACKGROUND: It is known that heparinoid, a mucopolysaccharide polysulfate, is effective in improving rough skin and promoting blood circulation as medicines for diseased areas. However, heparinoid has a molecular weight of more than 5000 and cannot penetrate healthy stratum corneum.
OBJECTIVE: We tested the efficacy of sulfated oligosaccharides with a molecular weight of less than 2000 on the human skin barrier function and
moisturizing function.
METHODS: We measured the transepidermal water loss (TEWL) of a three-dimensional human epidermis model cultured for 3 days after topical application of sulfated oligosaccharides, then observed the effects on TEWL suppression. The mRNA levels of proteins involved in intercellular lipid transport and storage in the stratum corneum, and moisture retention were measured using RT-qPCR.
RESULTS: An increase in the mRNA levels of the ATP-binding cassette subfamily A member 12 (ABCA12), which transports lipids into stratum granulosum, was confirmed. Increases were also observed in the mRNA levels of filaggrin (FLG), which is involved in the generation of natural
moisturizing factors, and of caspase-14, calpain-1 and bleomycin hydrolase, which are involved in the degradation of FLG. Antibody staining confirmed that the application of sodium trehalose sulfate to 3D model skin resulted in more ABCA12, ceramide, transglutaminase1, and FLG than those in controls. In a randomized, placebo-controlled, double-blind study, participants with low stratum corneum water content applied a lotion and emulsion containing sodium trehalose sulfate to their faces for 4 weeks. Sodium trehalose sulfate decreased the TEWL and increased the stratum corneum water content.
CONCLUSIONS: These results suggest that cosmetics containing sodium trehalose sulfate act on the epidermis by increasing barrier factors and
moisturizing factors, thereby ameliorating dry skin.