富硒绿豆(Se-MB)是绿豆(MB)和硒(Se)的组合,具有多种潜在的生物活性。然而,对Se-MB的护肤活性知之甚少。分析了Se-MB发酵液(Se-MBFB)的化学成分,保湿,Se-MBFB的抗衰老活性。酪氨酸酶抑制,黑素细胞(B16F10细胞)的抗黑色素生成,分析了人真皮成纤维细胞(HDFs)的保湿效果。此外,在体外评估Se-MBFB的自由基清除活性。为了验证Se-MBFB的体内作用和实际应用的潜力,对参与者进行了一项临床试验:31名年龄在25-60岁的中国女性,没有色素沉着障碍,没有疾病,没有过敏反应史,并且不使用护肤品在脸上。参与者在清洁面部后使用Se-MBFB面膜15-20分钟。测量点为使用面膜后的第0、2和4周(W0、W2和W4)。目标部位是脸颊和眼角。每次访问时在目标部位记录以下参数:黑色素指数,肤色,角质层水分含量,经皮水分流失,和乌鸦的脚。结果表明,Se-MBFB富含多酚,肽,和γ-氨基丁酸(GABA),表现出显著的自由基清除和酪氨酸酶抑制活性,减少黑色素的合成,并上调水通道蛋白3(AQP3)的表达。Se-MBFB面罩的测试表明,在使用Se-MBFB面罩4周后,参与者的脸变得更白,皱纹减少,水分含量增加。Se-MB具有优异的美白,保湿,和抗氧化功效,为Se-MB护肤品及其相关工业化妆品的利用和开发奠定了科学依据。
Selenium-enriched mung bean (Se-MB) is a combination of mung bean (MB) and selenium (Se), which have a variety of potential biological activities. However, little is known about the skincare activity of Se-MB. The chemical composition of Se-MB fermentation broth (Se-MBFB) was analyzed to investigate the whitening,
moisturizing, and anti-aging activities of Se-MBFB. The tyrosinase inhibition, anti-melanogenic in melanocytes (B16F10 cells), and
moisturizing effect in human dermal fibroblasts (HDFs) were analyzed. Besides, the free radical scavenging activity of Se-MBFB was assessed in vitro. To verify the in vivo effects and the potential of practical applications of Se-MBFB, a clinical trial was conducted on the participants: 31 Chinese women aged 25-60 years, with no pigmentation disorder, no illness, no history of hypersensitivity reaction, and no use of skincare product on the face. The participants used an Se-MBFB masque for 15-20 min after cleaning the face. The measurement points were Week 0, 2, and 4 (W0, W2, and W4) after using the masque, and target sites were cheek and canthus. The following parameters were recorded on the target sites at each visit: melanin index, skin color, cuticle moisture content, transepidermal water loss, and crow\'s feet. The results demonstrated that Se-MBFB was rich in polyphenols, peptides, and γ-aminobutyric acid (GABA), displayed significant free radical scavenging and tyrosinase inhibiting activities, decreased the synthesis of melanin, and upregulated the aquaporin-3 (AQP3) expression. The test of the Se-MBFB mask showed that after 4 weeks of using the Se-MBFB facemask, the faces of the participants became whiter with reduced wrinkles and increased moisture content. Se-MB possessed the excellent whitening,
moisturizing, and antioxidant efficacy, which could lay a scientific foundation for utilization and development of skincare products of Se-MB and its related industrial cosmetics products.