PDF(Pubmed)
Abstract：
Red rice, a variety of pigmented grain, serves dual purposes as both a food and medicinal resource. In recent years, we have witnessed an increasing interest in the dermatological benefits of fermented rice extracts, particularly their whitening and hydrating effects. However, data on the skincare advantages derived from fermenting red rice with Aspergillus oryzae remain sparse. This study utilized red rice as a substrate for fermentation by Aspergillus oryzae, producing a substance known as red rice Aspergillus oryzae fermentation (RRFA). We conducted a preliminary analysis of RRFA\'s composition followed by an evaluation of its skincare potential through various in vitro tests. Our objective was to develop a safe and highly effective skincare component for potential cosmetic applications. RRFA\'s constituents were assessed using high-performance liquid chromatography (HPLC), Kjeldahl nitrogen determination, the phenol-sulfuric acid method, and enzyme-linked immunosorbent assay (ELISA). We employed human dermal fibroblasts (FB) to assess RRFA\'s anti-aging and antioxidative properties, immortalized keratinocytes (HaCaT cells) and 3D epidermal models to examine its moisturizing and reparative capabilities, and human primary melanocytes (MCs) to study its effects on skin lightening. Our findings revealed that RRFA encompasses several bioactive compounds beneficial for skin health. RRFA can significantly promote the proliferation of FB cells. And it markedly enhances the mRNA expression of ECM-related anti-aging genes and reduces reactive oxygen species production. Furthermore, RRFA significantly boosts the expression of Aquaporin 3 (AQP3), Filaggrin (FLG), and Hyaluronan Synthase 1 (HAS1) mRNA, alongside elevating moisture levels in a 3D epidermal model. Increases were also observed in the mRNA expression of Claudin 1 (CLDN1), Involucrin (IVL), and Zonula Occludens-1 (ZO-1) in keratinocytes. Additionally, RRFA demonstrated an inhibitory effect on melanin synthesis. Collectively, RRFA contains diverse ingredients which are beneficial for skin health and showcases multifaceted skincare effects in terms of anti-aging, antioxidant, moisturizing, repairing, and whitening capabilities in vitro, highlighting its potential for future cosmetic applications.
摘要：
红米,各种有色谷物，作为食品和药用资源具有双重用途。近年来,我们看到人们对发酵大米提取物的皮肤病学益处越来越感兴趣，特别是它们的美白和补水效果。然而,关于用米曲霉发酵红米的护肤优势的数据仍然很少。这项研究利用红米作为米曲霉发酵的底物，生产一种被称为红米曲霉发酵（RRFA）的物质。我们对RRFA的成分进行了初步分析，然后通过各种体外测试评估了其护肤潜力。我们的目标是为潜在的化妆品应用开发安全有效的护肤成分。使用高效液相色谱法(HPLC)评估RRFA的成分，凯氏定氮测定,苯酚-硫酸法,和酶联免疫吸附测定(ELISA)。我们采用人真皮成纤维细胞（FB）来评估RRFA的抗衰老和抗氧化特性，永生化角质形成细胞（HaCaT细胞）和3D表皮模型，以检查其保湿和修复能力，和人原代黑素细胞（MCs），以研究其对皮肤美白的影响。我们的发现表明，RRFA包含几种对皮肤健康有益的生物活性化合物。RRFA能显著增进FB细胞的增殖。并且它显着增强ECM相关抗衰老基因的mRNA表达并减少活性氧的产生。此外，RRFA显著提高水通道蛋白3（AQP3）的表达，Filaggrin(FLG),和透明质酸合成酶1(HAS1)mRNA，同时提高3D表皮模型中的水分含量。在紧密连接蛋白1（CLDN1）的mRNA表达中也观察到增加，卷起蛋白(IVL),角质形成细胞中的ZonulaOccludens-1（ZO-1）。此外,RRFA显示了对黑色素合成的抑制作用。总的来说，RRFA包含多种成分，对皮肤健康有益，并在抗衰老方面展示了多方面的护肤效果，抗氧化剂，保湿,修复,和体外美白能力，突出了其未来化妆品应用的潜力。
