由于超声空化引起的独特反应条件,超声辅助调节生物材料的性能引起了越来越多的关注。在这项研究中,我们探索了通过离子液体体系超声喷雾纺丝法制备野生蚕丝纳米纤维膜,通过扫描电子显微镜(SEM)表征,傅里叶变换红外光谱(FTIR),X射线粉末衍射(XRD),差示扫描量热法(DSC),热重分析(TGA),原子力显微镜(AFM),水接触角,细胞相容性试验,和酶降解研究。我们研究了超声波在离子液体中传播对形态的影响,结构,热和机械性能,表面亲水性,生物相容性,和制造纤维的生物降解性。结果表明,随着超声处理时间从0min增加到60min,再生蚕丝纤维直径减小了0.97μm,比表面积增加了30.44μm2,提高了纤维表面的光滑度和均匀性。超声还促进了蛋白质分子链的重排和无序蛋白质结构向β-折叠的转化,将β-折叠含量提高到54.32%,这显著提高了材料的热稳定性(分解温度上升到256.38°C)和机械性能(弹性模量达到0.75GPa)。此外,亲水性,细胞相容性,和纤维膜的生物降解性都随着更长的超声暴露而改善,强调超声技术在促进天然生物聚合物在可持续材料科学和组织再生中应用的潜力。
Ultrasound-assisted regulation of biomaterial properties has attracted increasing attention due to the unique reaction conditions induced by ultrasound cavitation. In this study, we explored the fabrication of wild tussah silk nanofiber membranes via ultrasound spray spinning from an ionic liquid system, characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), atomic force microscopy (AFM), water contact angle, cytocompatibility tests, and enzymatic degradation studies. We investigated the effects of ultrasound propagation in an ionic liquid on the morphology, structure, thermal and mechanical properties, surface hydrophilicity, biocompatibility, and biodegradability of the fabricated fibers. The results showed that as ultrasound treatment time increased from 0 to 60 min, the regenerated silk fiber diameter decreased by 0.97 μm and surface area increased by 30.44 μm2, enhancing the fiber surface smoothness and uniformity. Ultrasound also promoted the rearrangement of protein molecular chains and transformation of disordered protein structures into β-sheets, increasing the β-sheet content to 54.32 %, which significantly improved the materials\' thermal stability (with decomposition temperatures rising to 256.38 °C) and mechanical properties (elastic modulus reaching 0.75 GPa). In addition, hydrophilicity, cytocompatibility, and biodegradability of the fiber membranes all improved with longer ultrasound exposure, highlighting the potential of ultrasound technology in advancing the properties of natural biopolymers for applications in sustainable materials science and tissue regeneration.