Abstract：
Ionic liquids (ILs), earlier praised for their eco-friendliness, have emerged as key chemicals in advancing green chemistry, catalysis, solvent development, and more. However, the discovery of their notable toxicity has led to a controversial reputation of ILs and has shifted the research landscape towards understanding their biological impacts. The present study examines the mechanism of cytotoxicity of 32 ILs across six classes, highlighting their effects on the cell cycle of the Jurkat cell line. Focusing on five ILs with pronounced cytotoxicity, we uncover their genotoxic effects and their role in inducing apoptosis. Our findings suggest intricate interplay between the extrinsic and intrinsic apoptotic pathways at different time points after exposure to ILs. Moreover, the ILs studied displayed marked genotoxicity, likely stemming from the accumulation of double-strand DNA breaks in the Jurkat cells. This investigation offers a comprehensive view on interactions of ILs with eukaryotic cells, thereby providing new guidelines for developing safer pharmaceutical and industrial applications of these chemicals. The results not only broaden and enhance the previous perceptions but also open new avenues in research, emphasizing the dual potential of ILs in innovation and safety, and marking a significant step towards integrating chemical innovations with biological safety.
摘要：
离子液体(IL),早些时候称赞他们的生态友好，已经成为推进绿色化学的关键化学品，催化作用,溶剂开发，还有更多.然而,其显著毒性的发现导致了IL的有争议的声誉，并使研究领域转向理解其生物学影响。本研究检查了6类32种IL的细胞毒性机制，强调它们对Jurkat细胞系细胞周期的影响。专注于五种具有明显细胞毒性的IL，我们揭示了它们的基因毒性作用以及它们在诱导细胞凋亡中的作用。我们的发现表明，在暴露于IL后的不同时间点，外在和内在凋亡途径之间存在复杂的相互作用。此外，研究的IL显示出明显的遗传毒性，可能源于Jurkat细胞中双链DNA断裂的积累。这项调查提供了关于IL与真核细胞相互作用的全面观点，从而为开发这些化学品的更安全的制药和工业应用提供了新的指导方针。研究结果不仅拓宽和增强了以往的认识,而且开辟了研究的新途径,强调IL在创新和安全方面的双重潜力，并标志着将化学创新与生物安全相结合的重要一步。
