背景:运动和糖的消耗导致肠屏障(IB)的功能障碍。这里,我们确定了在中度(A)和高强度耐力运动(B)后,天然基质中糖对肠道屏障的影响。
方法:在运行之前(前)和之后(后)确定IB功能,和120和180分钟后饮用通过测量血清内毒素浓度(脂多糖-LPS),IL-6、CD14和i-FABP。在研究A中,非专门训练的参与者(n=24,男性和女性,年龄26±4)以其个体无氧阈值(IAT)的80%跑一小时。完成后,跑步者消耗,在交叉设置中,500毫升的水,稀释的浑浊苹果汁(测试饮料),或不含果汁基质(FJM)的相同饮料(安慰剂)。在研究B中,参与者(n=30,男性和女性,50±9岁)完成了超马拉松比赛,被分成几组,并消耗了上述饮料之一。
结果:研究A:运动导致血清LPS显著增加,i-FABP,和IL-6,完成后下降很快。不同饮料对LPSi-FABP没有影响,或者可以观察到IL-6,但对CD14有影响。研究B:超马拉松导致血清LPS的强烈增加,在水和测试饮料组中完成后迅速下降,但安慰剂组没有。
结论:饮用的饮料不会影响适度运动后IB再生的动力学,但影响了CD14血清浓度,表明FJM对免疫系统可能的有益作用。在一场超级马拉松之后,IB函数再生非常快。糖(安慰剂)的摄入似乎对IB再生产生了负面影响,FJM的存在削弱了这一点。
BACKGROUND: Exercise and the consumption of sugars result in a dysfunction of the intestinal barrier (IB). Here, we determined the effect of sugar in a natural matrix on the intestinal barrier after moderate (A) and intensive endurance exercise (B).
METHODS: The IB function was determined before (pre) and after running (post), and 120 and 180 min after consuming the drink by measuring serum endotoxin concentrations (lipopolysaccharides-LPS), IL-6, CD14, and i-FABP. In study A, nonspecifically trained participants (n = 24, males and females, age 26 ± 4) ran for one hour at 80% of their individual anaerobic threshold (IAT). After finishing, the runners consumed, in a crossover setup, either 500 mL of water, diluted cloudy apple juice (test drink), or an identical drink (placebo) without the fruit juice matrix (FJM). In study B, the participants (n = 30, males and females, age 50 ± 9) completed an ultra-marathon run, were divided into groups, and consumed one of the above-mentioned drinks.
RESULTS: Study A: Exercise resulted in a significant increase in serum LPS, i-FABP, and IL-6, which decreased fast after finishing. No impact of the different drinks on LPS i-FABP, or IL-6 could be observed, but there was an impact on CD14. Study B: The ultra-marathon resulted in a strong increase in serum LPS, which decreased fast after finishing in the water and test drink groups, but not in the placebo group.
CONCLUSIONS: The consumed drinks did not affect the kinetics of IB regeneration after moderate exercise, but impacted CD14 serum concentrations, indicating possible beneficial effects of the FJM on the immune system. After an ultra-marathon, IB function regenerates very fast. The intake of sugar (placebo) seems to have had a negative impact on IB regeneration, which was diminished by the presence of the FJM.