用功能性材料作为添加剂喂养蚕是生产天然改性蚕丝,多样化,可控,和环境友好的方法,时间和投资成本低。在各种添加剂中,碳点(CD)由于其优异的生物相容性和荧光稳定性而显示出独特的优势。这里,合成了一种新型的绿色荧光碳点(G-CD),高油水分配比为147,低等电点为5.16,绝对量子产率为71%,和严格控制的表面状态。喂食G-CD后,蚕编织浅黄色茧,其绿色荧光在紫外光下肉眼可见。发光的丝绸缝在布上,以创建具有美丽荧光的醒目图案。这种G-CD对蚕的存活率和生命周期没有不利影响,并使其整个身体在紫外线下发光。基于强烈的荧光,化学稳定性,和生物安全,在消化道中发现了G-CD,丝腺,粪便,蚕茧,甚至是蛾的尸体.G-CD在分泌丝素蛋白的后部丝腺中积累,表明它与丝心蛋白的结合比丝胶蛋白更强,满足实际应用的要求。
Feeding silkworms with functional materials as additives to produce naturally modified silk is a facile, diverse, controllable, and environmentally friendly method with a low cost of time and investment. Among various additives, carbon dots (CDs) show unique advantages due to their excellent biocompatibility and fluorescence stability. Here, a new type of green fluorescent carbon dots (G-CDs) is synthesized with a high oil-water partition ratio of 147, a low isoelectric point of 5.16, an absolute quantum yield of 71%, and critically controlled surface states. After feeding with G-CDs, the silkworms weave light yellow cocoons whose green fluorescence is visible to the naked eye under UV light. The luminous silk is sewn onto the cloth to create striking patterns with beautiful fluorescence. Such G-CDs have no adverse effect on the survival rate and the life cycle of silkworms and enable their whole bodies to glow under UV light. Based on the strong fluorescence, chemical stability, and biological safety, G-CDs are found in the digestive tracts, silk glands, feces, cocoons, and even moth bodies. G-CDs accumulate in the posterior silk glands where fibroin protein is secreted, indicating its stronger combination with fibroin than sericin, which meets the requirements for practical applications.