■COVID-19大流行强调了全球微生物实验室生物安全的至关重要性。作为回应,中国加大了努力,加强其疾病控制和预防中心(CDC)实验室内的生物安全措施。这项研究首次对各省的生物安全实践进行了全面评估,城市,和县级CDC微生物实验室。
■我们从2021年到2023年进行了一项全国性的横断面调查,针对的是中国各级行政管理中心内微生物实验室的工作人员。采用分层抽样来选择受访者,确保跨不同CDC层次结构的代表性组合,职称,和学历。该调查包括有关生物安全培训的问题,BSL-2和BSL-3实验室的存在,遵守一般生物安全准则,以及关于标本的管理实践,试剂、和消耗品。进行统计分析以确定不同CDC水平之间生物安全实践的显着差异。
■共收到990份有效回复,强调了BSL-2实验室几乎普遍存在(98.69%),以及CDC网络中BSL-3实验室的显着但不同的存在。调查显示,生物安全培训水平很高(98.69%),并且遵守了生物安全协议。然而,在某些安全实践的一致应用中仍然存在挑战,特别是在较低的行政级别。在标本管理方面存在显著差异,试剂、和消耗品指出了在确保生物安全方面需要改进的领域。
■我们的研究结果表明,中国疾病预防控制中心微生物实验室的生物安全实践奠定了坚实的基础,反映了生物安全法实施后的重大进展。然而,遵守特定协议的可变性强调了持续培训的必要性,资源分配,和政策细化,以在各级统一提高生物安全标准。这项研究的见解对于指导实验室生物安全的未来改进至关重要,不仅在中国,而且可能在其他国家加强其公共卫生基础设施。
UNASSIGNED: The COVID-19 pandemic underscored the critical importance of
biosafety in microbiology laboratories worldwide. In response, China has ramped up its efforts to enhance
biosafety measures within its Centers for Disease Control and Prevention (CDC) laboratories. This study provides the first comprehensive assessment of
biosafety practices across provincial, city, and county levels of CDC microbiology laboratories in China.
UNASSIGNED: We conducted a nationwide cross-sectional survey from 2021 to 2023, targeting staff from microbiology laboratories within CDCs at all administrative levels in China. Stratified sampling was employed to select respondents, ensuring a representative mix across different CDC hierarchies, job titles, and academic qualifications. The survey encompassed questions on
biosafety training, the presence of BSL-2 and BSL-3 laboratories, adherence to general biosafety guidelines, and management practices regarding specimens, reagents, and consumables. Statistical analysis was performed to identify significant differences in biosafety practices among different CDC levels.
UNASSIGNED: A total of 990 valid responses were received, highlighting a nearly universal presence (98.69%) of BSL-2 laboratories and a significant yet varied presence of BSL-3 laboratories across the CDC network. The survey revealed high levels of
biosafety training (98.69%) and adherence to
biosafety protocols. However, challenges remain in the consistent application of certain safety practices, especially at lower administrative levels. Notable differences in the management of specimens, reagents, and consumables point to areas for improvement in ensuring biosecurity.
UNASSIGNED: Our findings indicate a robust foundation of biosafety practices within CDC microbiology laboratories in China, reflecting significant advancements in the wake of the Biosecurity Law\'s implementation. Nevertheless, the variability in adherence to specific protocols underscores the need for ongoing training, resources allocation, and policy refinement to enhance biosafety standards uniformly across all levels. This study\'s insights are crucial for guiding future improvements in laboratory biosafety, not just in China but potentially in other countries enhancing their public health infrastructures.