Abstract：
Alicyclobacillus bacteria are important contaminants in the beverage industry because their spores remain in the product after usual pasteurization. At the same time, their impact on human health has yet to be characterized, as it is generally assumed to be low or non-existent. However, these bacteria are causing quality concerns mainly due to odor and taste changes of the product. Since potential health effects are not precisely known, an experimental assessment was performed, including a biosafety assessment of six viable and non-viable vegetative and spore forms of Alicyclobacillus spp. strains using cell cultures and rodent study. The monolayer of Caco-2 (Cancer coli-2) cells was investigated for its adsorption effect on the epithelium of the small intestine of mice. Lactate dehydrogenase leakage (LDH) and transepithelial electrical resistance (TEER) tests were used to ensure the integrity of the cell membrane and tight junctions. The methylthiazole tetrazolium bromide (MTT) assay examined in vitro cytotoxicity in Caco-2 and HepG2 cell lines. The hemolysis of erythrocytes was spectrophotometrically measured. The results showed negligible cytotoxicity or non-toxic response in mice. In conclusion, Alicyclobacillus spp. exhibited biocompatibility with negligible cytotoxicity and minimal safety concerns.
摘要：
脂环族芽孢杆菌是饮料工业中的重要污染物,因为它们的孢子在常规巴氏灭菌后保留在产品中。同时,它们对人类健康的影响还有待表征，因为它通常被认为是低的或不存在的。然而,这些细菌主要由于产品的气味和味道变化而引起质量问题。由于潜在的健康影响尚不清楚，进行了实验评估，包括对六种可行和不可行的营养和孢子形式的脂环菌的生物安全性评估。使用细胞培养和啮齿动物研究的菌株。研究了单层Caco-2（Cancercoli-2）细胞对小鼠小肠上皮的吸附作用。乳酸脱氢酶泄漏（LDH）和跨上皮电阻（TEER）测试用于确保细胞膜和紧密连接的完整性。甲基噻唑溴化四唑（MTT）测定法检查了Caco-2和HepG2细胞系的体外细胞毒性。用分光光度法测量红细胞的溶血。结果显示小鼠的细胞毒性或无毒反应可忽略不计。总之,脂环杆菌。表现出生物相容性，细胞毒性可忽略不计，安全性问题最小。
