Abstract：
Risk assessment is the cornerstone of working safely with biological agents. The World Health Organization (WHO) Laboratory Biosafety Manual Fourth Edition Monograph on Risk Assessment provides stepwise guidance for completing a risk assessment, from information gathering and identifying hazards to evaluating the risks, developing, and implementing controls and review.To support the development of a mature safety culture within laboratories, it is important that all staff who handle biological agents understand the fundamentals of risk assessment and receive training in identifying hazards created by their work activities (or tasks) and understand how to mitigate the risks arising from carrying out that work. Any \"competent\" person may be involved in assessing the risks posed by carrying out an activity. Those closest to the work, who understand the details of the task being undertaken, should be involved in creating the risk assessment. The guidance in this chapter is not just applicable to biosafety professionals, laboratory scientists, or facility managers but can be used by any competent worker familiar with the activity being assessed.This chapter uses the guidance from the WHO to apply the principles of risk assessment to working with Epizootic hemorrhagic disease virus (EHDV), using an example activity-virus isolation from EHDV test samples in cell culture.
摘要：
风险评估是安全使用生物制剂的基石。世界卫生组织（WHO）实验室生物安全手册第四版风险评估专论为完成风险评估提供了逐步指导，从信息收集和识别危险到评估风险，发展,并实施控制和审查。支持在实验室内发展成熟的安全文化，重要的是，所有处理生物制剂的工作人员都必须了解风险评估的基础知识，并接受识别其工作活动(或任务)造成的危险的培训，并了解如何减轻因开展这项工作而产生的风险。任何“有能力的”人员都可能参与评估因开展活动而带来的风险。那些最接近工作的人，他们了解正在执行的任务的细节，应该参与创建风险评估。本章中的指南不仅适用于生物安全专业人员，实验室科学家,或设施经理，但可以由任何熟悉被评估活动的合格工人使用。本章使用世界卫生组织的指南，将风险评估的原则应用于流行病出血性疾病病毒（EHDV）的工作，使用示例活性-从细胞培养物中的EHDV测试样品中分离病毒。
