PDF(Pubmed)
Abstract：
The escalating elderly population worldwide has prompted a surge of interest in longevity medicine. Its goal is to interfere with the speed of ageing by slowing it down or even reversing its accompanying effects. As a field, it is rapidly growing and spreading into different branches. One of these is the use of nutraceuticals as anti-ageing drugs. This field is gaining massive popularity nowadays, as people are shifting towards a more natural approach to life and seeking to use natural products as a source of medicine. The present article focuses on the cellular effect of Haberlea rhodopensis Friv. in vitro culture total ethanol extract (HRT), produced by a sustainable biotechnological approach. The extract showed a similar phytochemical profile to plant leaf extract and was rich in primary bioactive ingredients-caffeoyl phenylethanoid glycosides, myconoside, and paucifloside. This study examined the biosafety potential, cytotoxicity, genotoxicity, and mitochondrial activity of the extract using in vitro cultures. The results showed high cell survival rates and minimal cytotoxic effects on Lep3 cells, with no induction of reactive oxygen species nor genotoxicity. Additionally, the extract positively influenced mitochondrial activity, indicating potential benefits for cellular health. The results are promising and show the beneficial effect of HRT without the observation of any adverse effects, which sets the foundation for its further testing and potential therapeutic applications.
摘要：
全球不断增长的老年人口促使人们对长寿医学的兴趣激增。它的目标是通过减慢衰老速度甚至逆转其伴随效应来干扰衰老速度。作为一个领域，它迅速生长并扩散到不同的分支。其中之一是使用营养食品作为抗衰老药物。这个领域现在越来越受欢迎，随着人们转向更自然的生活方式，并寻求使用天然产品作为药物来源。本文重点介绍了HaberlearhodopensisFriv的细胞作用。体外培养总乙醇提取物（HRT），通过可持续的生物技术方法生产。该提取物显示出与植物叶提取物相似的植物化学特征，并且富含主要的生物活性成分-咖啡酰基苯乙醇苷，Myconoside,和paucifloside。这项研究检查了生物安全的潜力，细胞毒性，遗传毒性，和使用体外培养的提取物的线粒体活性。结果显示对Lep3细胞的高细胞存活率和最小的细胞毒性作用，没有活性氧的诱导也没有遗传毒性。此外,提取物对线粒体活性有积极影响，表明对细胞健康的潜在益处。结果是有希望的，并显示了HRT的有益效果，而没有观察到任何不利影响，这为其进一步的测试和潜在的治疗应用奠定了基础。
