维罗妮卡物种(speedwells)的提取物以其显示的各种生物活性而闻名,如细胞毒性,抗菌,抗炎,和抗氧化活性。此外,该属植物在世界范围内被称为传统医学中使用的药用植物。酚类化合物是对生物活性贡献最大的专门代谢物。因此,这项研究的目的是鉴定和定量三种Veronica物种中存在的酚类化合物(Veronicaanagallis-aquaticaL.,VeronicaPersicaPoir.,和VeronicapolitaFr.)使用液相色谱-质谱(LC-MS/MS)技术。用两种方法测试所有提取物的抗氧化活性:DPPH(2,2-二苯基-1-吡啶酰肼)和ORAC(氧自由基吸收能力)。此外,还测试了在所有物种中检测到的最高量的化合物的标准物的抗氧化活性。三种不同的溶剂(纯甲醇,80%乙醇,和水)用于酚类成分的提取及其比较,以测试其抗氧化活性作为最终目标。所测试的维罗妮卡提取物中存在的主要化合物是:对羟基苯甲酸,香草酸,咖啡酸,龙胆酸,还有芹菜素.与其他两个测试物种相比,anagallis-aquatica含有最高含量的酚类成分,V.Persica和V.Polita.咖啡酸在两种研究方法中均显示出最高的抗氧化活性,DPPH活性的IC50值为1.99µg/mL。对于植物提取物,总的来说,甲醇/乙醇提取物在两种方法中均显示出比水提取物更高的活性,作为有机溶液提取更多的酚类化合物。这项研究指出了不同维罗妮卡物种提取物在抗氧化活性方面的潜在应用。
Extracts from
Veronica species (speedwells) are known for the various biological activities they show, such as cytotoxic, antimicrobial, anti-inflammatory, and antioxidant activities. Also, the plants from this genus are known as medicinal plants used in traditional medicine worldwide. Phenolic compounds are specialized metabolites that contribute to biological activity the most. Therefore, the aim of this research is identification and quantification of phenolic compounds present in three
Veronica species (
Veronica anagallis-aquatica L.,
Veronica persica Poir., and
Veronica polita Fr.) using the liquid chromatography-mass spectrometry (LC-MS/MS) technique. All extracts were tested for antioxidant activity with two methods: DPPH (2,2-diphenyl-1-picrylhydrazyl) and ORAC (oxygen radical absorbance capacity). Also, standards for compounds that were detected in the highest amount in all species were also tested for antioxidant activity. Three different solvents (pure methanol, 80% ethanol, and water) were used for the extraction of phenolic components and their comparison in order to test their antioxidant activity as a final goal. The main compounds present in the tested
Veronica extracts were: p-hydroxybenzoic acid, vanillic acid, caffeic acid, gentisic acid, and apigenin. V. anagallis-aquatica contained the highest amount of phenolic components in comparison with the two other tested species, V. persica and V. polita. Caffeic acid showed the highest antioxidant activity in both studied methods with an IC50 value for DPPH activity of 1.99 µg/mL. For the plant extracts, in general, methanolic/ethanolic extracts showed higher activity than water extracts in both methods which was expected, as organic solutions extract more phenolic compounds. This research points to the potential application of extracts of different
Veronica species for antioxidant activity.