PDF(Pubmed)
Abstract：
Fluorescence in situ hybridization (FISH), a molecular cytogenetic technique that enables the visualization and identification of specific DNA sequences within chromosomes, has emerged as a pivotal tool in plant breeding programs, particularly in the case of Veronica species. Veronica, a genus with a complex reproductive system, often poses challenges in accurately identifying hybrids because of its tendency to hybridize, which leads to intricate genetic variation. This study focused on the use of FISH as a prescreening method to identify true hybrids in Veronica breeding programs. FISH analysis was first performed on the parents to identify their 45S and 5S rDNA signals, along with their respective chromosome numbers. The signals were then compared with those of the twenty progenies with reference to their supposed parents. Five true hybrids, seven self-pollinated progenies, and eight false hybrids were identified through FISH. The findings highlight the significance of FISH as a screening method that contributes significantly to the efficiency of Veronica breeding programs by ensuring the preservation of desired genetic traits and minimizing the inadvertent inclusion of misidentified hybrids. To conclude, this study underscores the vital role of FISH in enhancing the precision and success of breeding programs and opens new avenues for improved breeding strategies and crop development.
摘要：
荧光原位杂交(FISH),一种分子细胞遗传学技术，能够可视化和鉴定染色体内的特定DNA序列，已经成为植物育种计划的关键工具，特别是在维罗妮卡物种的情况下。维罗妮卡,具有复杂生殖系统的属，由于杂种的杂交倾向，在准确识别杂种时往往会带来挑战，导致复杂的遗传变异。这项研究的重点是使用FISH作为预筛选方法来鉴定Veronica育种计划中的真正杂种。首先对父母进行FISH分析，以鉴定他们的45S和5SrDNA信号，以及它们各自的染色体数量。然后将信号与二十个后代的信号进行比较，并参考其假定的父母。五个真正的混血儿，七个自花授粉的后代，通过FISH鉴定出8个假杂种。研究结果强调了FISH作为一种筛选方法的重要性，该方法通过确保保留所需的遗传性状并最大程度地减少错误识别的杂种的无意包含，从而显着提高了Veronica育种计划的效率。最后，这项研究强调了FISH在提高育种计划的准确性和成功率方面的重要作用，并为改进育种策略和作物发育开辟了新的途径。
