■患有终末期肾病(ESRD)的血液透析患者容易感染和菌群失调。导管相关感染通常由机会性皮肤病原体引起。这项研究旨在比较隧道袖口导管(导管周围组)和对侧部位(对照组)出口部位周围的皮肤微生物群变化。
■招募接受血液透析的ESRD患者。用潮湿的皮肤拭子收集皮肤微生物群,并使用16SrDNAV3-V4区域的高通量测序进行分析。去噪之后,去复制,去除嵌合体,读数被分配到零半径操作分类单位(ZOTU).
■我们发现,与对照组相比,导管周组的α多样性显着降低,正如香农所指出的,约斯特,和公平性指数,但不是通过Chao1或丰富度指数。Beta多样性分析显示,导管周围的微生物区系与其相应的对照组存在显着差异。Firmicutes的代表过多,放线菌的代表不足,变形杆菌,和酸性细菌在导管周围组的门水平。最丰富的ZOTU(葡萄球菌属。)急剧增加,而Cutibacterium,一种共生细菌,在导管周围组下降。网络分析显示,皮肤微生物群显示出与局部和生化因素的协方差。
■总而言之,与对照部位相比,ESRD透析患者的出口部位存在显著的皮肤微生物群失调.管理皮肤菌群失调是预防导管相关细菌感染的有希望的目标。
UNASSIGNED: Hemodialysis patients with end-stage renal disease (ESRD) are susceptible to infections and dysbiosis. Catheter-related infections are typically caused by opportunistic skin pathogens. This study aims to compare the skin microbiota changes around the exit site of tunneled cuffed catheters (peri-catheter group) and the contralateral site (control group).
UNASSIGNED: ESRD patients on hemodialysis were recruited. The skin microbiota were collected with moist skin swabs and analyzed using high-throughput sequencing of the 16S rDNA V3-V4 region. After denoising, de-replication, and removal of chimeras, the reads were assigned to zero-radius operational taxonomic units (ZOTU).
UNASSIGNED: We found significantly reduced alpha diversity in the peri-catheter group compared to the control group, as indicated by the Shannon, Jost, and equitability indexes, but not by the Chao1 or richness indexes. Beta diversity analysis revealed significant deviation of the peri-catheter microbiota from its corresponding control group. There was an overrepresentation of Firmicutes and an underrepresentation of Actinobacteria, Proteobacteria, and Acidobacteria at the phylum level in the peri-catheter group. The most abundant ZOTU (
Staphylococcus spp.) drastically increased, while Cutibacterium, a commensal bacterium, decreased in the peri-catheter group. Network analysis revealed that the skin microbiota demonstrated covariance with both local and biochemical factors.
UNASSIGNED: In conclusion, there was significant skin microbiota dysbiosis at the exit sites compared to the control sites in ESRD dialysis patients. Managing skin dysbiosis represents a promising target in the prevention of catheter-related bacterial infections.