PDF(Pubmed)
Abstract：
Examination of bacteria/host cell interactions is important for understanding the aetiology of many infectious diseases. The colony forming unit (CFU) has been the standard for quantifying bacterial burden for the past century, however, this suffers from low sensitivity and is dependent on bacterial culturability in vitro. Our data demonstrate the discrepancy between the CFU and bacterial genome copy number in an osteomyelitis-relevant co-culture system and we confirm diagnosis and quantify bacterial load in clinical bone specimens. This study provides an improved workflow for the quantification of bacterial burden in such cases.
摘要：
检查细菌/宿主细胞相互作用对于了解许多传染病的病因很重要。在过去的一个世纪中，菌落形成单位（CFU）一直是量化细菌负担的标准。然而,这具有低敏感性，并且依赖于体外细菌的可培养性。我们的数据表明，在骨髓炎相关的共培养系统中，CFU和细菌基因组拷贝数之间存在差异，我们确认了诊断并量化了临床骨标本中的细菌负荷。这项研究为在这种情况下量化细菌负荷提供了改进的工作流程。
